2019
DOI: 10.3390/v11090841
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Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms

Abstract: Salmonella contamination in foods and their formation of biofilms in food processing facility are the primary bacterial cause of a significant number of foodborne outbreaks and infections. Broad lytic phages are promising alternatives to conventional technologies for pathogen biocontrol in food matrices and reducing biofilms. In this study, 42 Salmonella phages were isolated from environmentally-sourced water samples. We characterized the host range and lytic capacity of phages LPSTLL, LPST94 and LPST153 again… Show more

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Cited by 117 publications
(125 citation statements)
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“…The efficiency of selected phages virulence were evaluated respectively by lysing S. Enteritidis SGSC 4901, as published previously [27]. In brief, the experimental group was set up by infecting 100 µL freshly cultured S. Enteritidis SGSC 4901 (7 log 10 CFU/mL) with 100 µL of diluted phage suspension (5 log 10 PFU/mL) in a clear 96-well microplate (Corning Incorporated, USA).…”
Section: Salmonella Challenge Testsmentioning
confidence: 99%
“…The efficiency of selected phages virulence were evaluated respectively by lysing S. Enteritidis SGSC 4901, as published previously [27]. In brief, the experimental group was set up by infecting 100 µL freshly cultured S. Enteritidis SGSC 4901 (7 log 10 CFU/mL) with 100 µL of diluted phage suspension (5 log 10 PFU/mL) in a clear 96-well microplate (Corning Incorporated, USA).…”
Section: Salmonella Challenge Testsmentioning
confidence: 99%
“…In our study, killing assay exhibited the host strain EF3964 can be inhibited or killed at different MOI for 12 h in medium. Potential bactericidal ability at low MOI was similar to those of the single phage 13076 and phage 14028 [26]. In vegetable model, PHB08 can kill 1⋅10 5 CFU/cm 2 E. faecalis at 25 °C for 24 h. It can be inferred that phage-based biological control methods have great potential in improving the safety of food microorganisms.…”
Section: Killing Of Phb08 In Vitromentioning
confidence: 66%
“…The lytic activity of phage PHB08 was analyzed in a 96-well microtiter plate by examining the optical density measurement method [26,27]. Brie y, 100 µl of the mid-log phase E. faecalis EF3964 (6.6⋅10 7 Colony Forming Units, CFU) mixed with 100 µl of phage PHB08 of different MOI (0.001, 0.01, 0.1, 1.0, 10, 100, and 1000) was incubated at 37 °C (160 rpm).…”
Section: Lytic Activity Of Phb08mentioning
confidence: 99%
“…A total of 40 putative different phages were isolated from the environmentally sourced water samples collected in Wuhan, China in accordance with previously described methods [34,35]. Enrichment for phage isolation was modified from previously published methods [36].…”
Section: Enrichment Isolation Purification and Preparation Of Phagesmentioning
confidence: 99%
“…Lytic activity experiments were performed according to previously described method [35,37]. In brief, 100 µL fresh overnight cultures of Salmonella (7 log 10 CFU/mL) were added to 100 µL of phage suspensions (MOIs 100, 10, 1, and 0.1) in a 96 well plate.…”
Section: Lytic Activity Of Phage Lpst94mentioning
confidence: 99%