Phage-based biocontrol strategies to reduce foodborne pathogens in foods

Goodridge, Lawrence; Bisha, Bledar

doi:10.4161/bact.1.3.17629

Cited by 161 publications

(129 citation statements)

References 47 publications

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“…Bacteriophages have a long history of use in Eastern European countries (10), and phage-based biocontrol of food-borne pathogens is a promising approach (11). Their use for reducing Campylobacter in the chicken gut has been investigated in studies with different phages, doses, experimental settings, and application routes.…”

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confidence: 99%

Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Kittler¹,

Fischer²,

Abdulmawjood

et al. 2013

Appl Environ Microbiol

102

121

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Campylobacteriosis is the most frequent food-borne human enteritis. The major source for infection with Campylobacter spp. is broiler meat. Risk assessments consider the reduction of Campylobacter in primary production to be most beneficial for human health. The aim of this study was to test the efficacy of a bacteriophage application under commercial conditions which had proved to be effective in previous noncommercial studies under controlled experimental conditions. A phage cocktail for Campylobacter reduction was tested on three commercial broiler farms each with a control and an experimental group. Colonization of Campylobacter was confirmed prior to phage application in fecal samples. Subsequently, a phage cocktail was applied via drinking water in the experimental group (log 10 5.8 to 7.5 PFU/bird). One day after phage application, Campylobacter counts of one experimental group were reduced under the detection limit (<50 CFU/g, P ‫؍‬ 0.0140) in fecal samples. At slaughter, a significant reduction of >log 10 3.2 CFU/g cecal content compared to the control was still detected (P ‫؍‬ 0.0011). No significant reduction was observed in the experimental groups of the other trials. However, a significant drop in cecal Campylobacter counts occurred in a phage-contaminated control. These results suggest that maximum reduction of Campylobacter at the slaughterhouse might be achieved by phage application 1 to 4 days prior to slaughter.

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confidence: 99%

Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Kittler¹,

Fischer²,

Abdulmawjood

et al. 2013

Appl Environ Microbiol

102

121

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“…Since their discovery in the early 1900s, phages have been used as therapeutic agents in human infections (8,9) but also as alternative biocontrol agents in agriculture, sewage treatment, and the food industry (10)(11)(12)(13). Furthermore, phage display technology has proved useful in cancer studies and for the delivery of vaccines (12,13).…”

mentioning

confidence: 99%

Bacteriophage ϕMAM1, a Viunalikevirus, Is a Broad-Host-Range, High-Efficiency Generalized Transducer That Infects Environmental and Clinical Isolates of the Enterobacterial Genera Serratia and Kluyvera

Matilla

Salmond

2014

Appl Environ Microbiol

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Members of the enterobacterial genus Serratia are ecologically widespread, and some strains are opportunistic human pathogens. Bacteriophage MAM1 was isolated on Serratia plymuthica A153, a biocontrol rhizosphere strain that produces the potently bioactive antifungal and anticancer haterumalide oocydin A. The MAM1 phage is a generalized transducing phage that infects multiple environmental and clinical isolates of Serratia spp. and a rhizosphere strain of Kluyvera cryocrescens. Electron microscopy allowed classification of MAM1 in the family Myoviridae. Bacteriophage MAM1 is virulent, uses capsular polysaccharides as a receptor, and can transduce chromosomal markers at frequencies of up to 7 ؋ 10 ؊6 transductants per PFU. We also demonstrated transduction of the complete 77-kb oocydin A gene cluster and heterogeneric transduction of a plasmid carrying a type III toxin-antitoxin system. These results support the notion of the potential ecological importance of transducing phages in the acquisition of genes by horizontal gene transfer. Phylogenetic analyses grouped MAM1 within the ViI-like bacteriophages, and genomic analyses revealed that the major differences between MAM1 and other ViI-like phages arise in a region encoding the host recognition determinants. Our results predict that the wider genus of ViI-like phages could be efficient transducing phages, and this possibility has obvious implications for the ecology of horizontal gene transfer, bacterial functional genomics, and synthetic biology.

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“…Bacteriophages or phages in animals and in food products have recently attracted broad interest as a novel biocontrol agent for foodborne pathogens Goodridge and Bisha, 2011 . Phages are bacterial viruses that infect and lyse target bacteria with high specificity.…”

Section: Isolation Of Campylobacter Bacteriophages From Chicken Meat mentioning

confidence: 99%

Characterization and Application of Lytic Bacteriophages against <i>Campylobacter</i> <i>jejuni</i> Isolated from Poultry in Japan

et al. 2017

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The aim was to isolate Campylobacter jejuni-specific lytic phages from meats on the market in Japan. These phages were effectively isolated from 13 of 15 86.7% retail chicken meat samples skin and liver by the enrichment method using Preston Campylobacter Selective Enrichment Broth and 10 host Campylobacter strains. Among the 26 phage isolates, 14 were extracted by means of C. jejuni L26 as a host strain. Phage PHC10 showed the broadest lytic spectrum: active against 67.4% of the 46 C. jejuni strains tested. The other phage isolates showed different lytic spectra. Because phages PHC5, PHC10, PHC19, PHC22, and PHC25 possess an icosahedral head and a contracted tail, they seem to be members of the Myoviridae family. Effects of 19 phage isolates on viability of C. jejuni were investigated. These phages reduced viable counts of C. jejuni by 1-3 log after 6-12 h of incubation at 42 as compared to the initial counts. The C. jejuni L26 was found to be suitable as a host because of the wide hosting range. The phages isolated in this study seem to be promising biocontrol agents against C. jejuni in food.

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Phage-based biocontrol strategies to reduce foodborne pathogens in foods

Cited by 161 publications

References 47 publications

Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Effect of Bacteriophage Application on Campylobacter jejuni Loads in Commercial Broiler Flocks

Bacteriophage ϕMAM1, a Viunalikevirus, Is a Broad-Host-Range, High-Efficiency Generalized Transducer That Infects Environmental and Clinical Isolates of the Enterobacterial Genera Serratia and Kluyvera

Characterization and Application of Lytic Bacteriophages against <i>Campylobacter</i> <i>jejuni</i> Isolated from Poultry in Japan

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