Phage delivered CRISPR-Cas system to combat multidrug-resistant pathogens in gut microbiome

Nath, Arijit; Bhattacharjee, Rahul; Nandi, Aditya; Sinha, Adrija; Kar, Sulagna; Manoharan, Nikita; Mitra, Shirsajit; Mojumdar, Abhik; Panda, Pritam Kumar; Patro, Swadheena; Dutt, A.; Ahuja, Rajeev; Verma, Suresh K.; Suar, Mrutyunjay

doi:10.1016/j.biopha.2022.113122

Cited by 32 publications

(22 citation statements)

References 110 publications

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“…MiRNA-dependent activity is primarily post-transcriptional that results in either total mRNA degradation or halting of translation machinery, which in both cases represses target gene expression [ 66 , 68 ]. Emerging pre-clinical and clinical evidence has established that miRNAs are secreted in bodily fluids suggestive of effective early stage biomarkers and therapeutics [ 69 , 70 ]. Currently employed detection methods such as, miRNA microarray or, miRNA/small RNA sequencing, probe-based in situ hybridization, northern blotting or, quantitative-polymerase chain reaction (qPCR), hybridization-based methods, surface plasmon resonance (SPR) imaging provide information depending on the expression levels of miRNA/s between tissues, body fluids or cellular systems between diseased and healthy controls [ 70 – 74 ].…”

Section: Application Of Functionalized Mnps In Pre-clinical and Clini...mentioning

confidence: 99%

Emerging trends in the nanomedicine applications of functionalized magnetic nanoparticles as novel therapies for acute and chronic diseases

et al. 2022

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High-quality point-of-care is critical for timely decision of disease diagnosis and healthcare management. In this regard, biosensors have revolutionized the field of rapid testing and screening, however, are confounded by several technical challenges including material cost, half-life, stability, site-specific targeting, analytes specificity, and detection sensitivity that affect the overall diagnostic potential and therapeutic profile. Despite their advances in point-of-care testing, very few classical biosensors have proven effective and commercially viable in situations of healthcare emergency including the recent COVID-19 pandemic. To overcome these challenges functionalized magnetic nanoparticles (MNPs) have emerged as key players in advancing the biomedical and healthcare sector with promising applications during the ongoing healthcare crises. This critical review focus on understanding recent developments in theranostic applications of functionalized magnetic nanoparticles (MNPs). Given the profound global economic and health burden, we discuss the therapeutic impact of functionalized MNPs in acute and chronic diseases like small RNA therapeutics, vascular diseases, neurological disorders, and cancer, as well as for COVID-19 testing. Lastly, we culminate with a futuristic perspective on the scope of this field and provide an insight into the emerging opportunities whose impact is anticipated to disrupt the healthcare industry. Graphical Abstract

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Section: Application Of Functionalized Mnps In Pre-clinical and Clini...mentioning

confidence: 99%

Emerging trends in the nanomedicine applications of functionalized magnetic nanoparticles as novel therapies for acute and chronic diseases

et al. 2022

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“…Phage therapy is an example of an innovative CRISPR technology that genetically alters bacteriophages (the viruses that invade bacteria) to treat antibiotic-resistant bacterial infections. 8 The trick here is to introduce a CRISPR system in bacteriophages (the viruses that invade bacteria) so that when they infect the targeted antibiotic-resistant bacteria they will cut and dice the bacterial genome, terminate bacterial DNA replication, and stop the bacterial infection. Bacteriophages are highly specific pathogens for bacteria and do not normally infect human cells, making this procedure relatively safe to treat humans.…”

Section: Innovative Clinical Applications Of Crisprmentioning

confidence: 99%

CRISPR Technology: A Jewish Legal Perspective

Loike¹,

Blaum²

2022

Rambam Maimonides Med J

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Clustered regularly interspaced short palindromic repeats (CRISPR) gene editing is an innovative and potentially game-changing biotechnology that can potentially reverse DNA mutations in a tissue-specific manner. In addition, CRISPR is being targeted for xenotransplantation, for increasing human longevity, in animal breeding, and in plant science. However, there are many ethical challenges that emerge from CRISPR technology. This article discusses several positions that relate to these ethical challenges from a Jewish legal perspective. In addition, we present several other applications of CRISPR technology that lack a defined Jewish legal precedent and require rabbinical scholars to address and resolve them in the future.

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“…The state-of-the-art CRISPR/Cas9-based genome editing could be employed to target the microbes which cause pathogenesis in the host (Zheng et al, 2022). Polymorphisms in host receptors can further be exploited to understand the virulence potential of pathogenic bacteria that can be targeted for suppression by CRISPR-Cas9 based therapies (Ramachandran and Bikard, 2019;Nath et al, 2022).…”

Section: Possible Targets For Discoverymentioning

confidence: 99%

Play the plug: How bacteria modify recognition by host receptors?

2022

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The diverse microbial community that colonizes the gastrointestinal tract has remarkable effects on the host immune system and physiology resulting in homeostasis or disease. In both scenarios, the gut microbiota interacts with their host through ligand-receptor binding whereby the downstream signaling processes determine the outcome of the interaction as disease or the counteractive immune responses of the host. Despite several studies on microbe-host interactions and the mechanisms by which this intricate process happens, a comprehensive and updated inventory of known ligand-receptor interactions and their roles in disease is paramount. The ligands which originate as a result of microbial responses to the host environment contribute to either symbiotic or parasitic relationships. On the other hand, the host receptors counteract the ligand actions by mounting a neutral or an innate response. The varying degrees of polymorphic changes in the host receptors contribute to specificity of interaction with the microbial ligands. Additionally, pathogenic microbes manipulate host receptors with endogenous enzymes belonging to the effector protein family. This review focuses on the diversity and similarity in the gut microbiome-host interactions both in health and disease conditions. It thus establishes an overview that can help identify potential therapeutic targets in response to critically soaring antimicrobial resistance as juxtaposed to tardy antibiotic development research.

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Phage delivered CRISPR-Cas system to combat multidrug-resistant pathogens in gut microbiome

Cited by 32 publications

References 110 publications

Emerging trends in the nanomedicine applications of functionalized magnetic nanoparticles as novel therapies for acute and chronic diseases

Emerging trends in the nanomedicine applications of functionalized magnetic nanoparticles as novel therapies for acute and chronic diseases

CRISPR Technology: A Jewish Legal Perspective

Play the plug: How bacteria modify recognition by host receptors?

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