Phage display-based on-slide selection of tumor-specific antibodies on formalin-fixed paraffin-embedded human tissue biopsies

Haaf, Andre ten; Pscherer, Sibylle; Fries, Katharina; Barth, Stefan; Gattenlöhner, Stefan; Tur, Mehmet Kemal

doi:10.1016/j.imlet.2015.05.013

Cited by 13 publications

(12 citation statements)

References 50 publications

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“…Antibody selection performed on whole frozen sections with immune libraries [54][55][56] will not be discussed here because this review is focused on naïve libraries. The present section describes antibody selection on whole FFPE sections [57,58], and also three on-slide micro-selection strategies to target specific tissue structures or cell clusters (Figure 1). Two of them rely on the excision of the positive tissue by laser-assisted microdissection [59][60][61][62][63] or with a micropipette [64]; the third one blocks bacterial replication of irrelevant phages through UV-induced DNA damage [65][66][67][68][69][70][71].…”

Section: On-slide Antibody Selectionmentioning

confidence: 99%

“…All these strategies can be used on FFPE and frozen tissue sections. With the exception of the studies by ten Haaf et al [57,58] and by Sun et al [62], the antibody selection strategies described here include a phage display library panning step directly on slides where the rare cells of interest are surrounded by a large excess of other cells (Table 1, "Depletion"). The presence of these different cell types on the slides allows the simultaneous selection and depletion by competition.…”

Section: On-slide Antibody Selectionmentioning

confidence: 99%

“…To target cancer cells in their microenvironment, ten Haaf et al selected them directly on slides using lung cancer FFPE tissue sections from different patients and healthy lung tissue samples for the depletion step [57,58]. Each of the three rounds of panning was carried out on four slides, to test different epitope retrieval conditions.…”

Section: On Whole Ffpe Sectionsmentioning

confidence: 99%

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Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries

Mairaville¹,

Martineau²

2021

Antibodies

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Immunohistochemistry is a widely used technique for research and diagnostic purposes that relies on the recognition by antibodies of antigens expressed in tissues. However, tissue processing and particularly formalin fixation affect the conformation of these antigens through the formation of methylene bridges. Although antigen retrieval techniques can partially restore antigen immunoreactivity, it is difficult to identify antibodies that can recognize their target especially in formalin-fixed paraffin-embedded tissues. Most of the antibodies currently used in immunohistochemistry have been obtained by animal immunization; however, in vitro display techniques represent alternative strategies that have not been fully explored yet. This review provides an overview of phage display-based antibody selections using naïve antibody libraries on various supports (fixed cells, dissociated tissues, tissue fragments, and tissue sections) that have led to the identification of antibodies suitable for immunohistochemistry.

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Section: On-slide Antibody Selectionmentioning

confidence: 99%

Section: On-slide Antibody Selectionmentioning

confidence: 99%

Section: On Whole Ffpe Sectionsmentioning

confidence: 99%

See 1 more Smart Citation

Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries

Mairaville¹,

Martineau²

2021

Antibodies

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“…Antibody technology has been improved significantly by the advent of antibody-phage display libraries, which permits panning out of unique disease-specific antibody fragments for use as building blocks to construct fully functional antibodies and derivatives thereof [ 35 ]. This method has also enabled the selection of fully human single chain variable antibody fragments (scFv’s), some of which have been used to replace non-human antibody domains from conventional ADCs, thereby reducing the risk of immunogenicity when applied in humans.…”

Section: Cspg4 As Target For Cancer Therapymentioning

confidence: 99%

CSPG4: A Target for Selective Delivery of Human Cytolytic Fusion Proteins and TRAIL

et al. 2017

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Chondroitin-sulfate proteoglycan 4 (CSPG4) is a transmembrane glycoprotein overexpressed on malignant cells in several cancer types with only limited expression on normal cells. CSPG4 is implicated in several signaling pathways believed to drive cancer progression, particularly proliferation, motility and metastatic spread. Expression may serve as a prognostic marker for survival and risk of relapse in treatment-resistant malignancies including melanoma, triple negative breast cancer, rhabdomyosarcoma and acute lymphoblastic leukemia. This tumor-associated overexpression of CSPG4 points towards a highly promising therapeutic target for antibody-guided cancer therapy. Monoclonal αCSPG4 antibodies have been shown to inhibit cancer progression by blocking ligand access to the CSPG4 extracellular binding sites. Moreover, CSPG4-directed antibody conjugates have been shown to be selectively internalized by CSPG4-expressing cancer cells via endocytosis. CSPG4-directed immunotherapy may be approached in several ways, including: (1) antibody-based fusion proteins for the selective delivery of a pro-apoptotic factors such as tumor necrosis factor-related apoptosis-inducing ligand to agonistic death receptors 4 and 5 on the cell surface; and (2) CSPG4-specific immunotoxins which bind selectively to diseased cells expressing CSPG4, are internalized by them and induce arrest of biosynthesis, closely followed by initiation of apoptotic signaling. Here we review various methods of exploiting tumor-associated CSPG4 expression to improve targeted cancer therapy.

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“…The results of the study concluded the safety and specificity of the method in human subjects. Furthermore, phage display-extracted peptides are also used for the detection of cancer cells in biopsies samples [48]. 6.1 Thyroid Cancer: Carneiro, et al [49] reported a peptide using scFv phage library that had affinity to bind to papillary thyroid carcinoma proteins, OTU domain-containing protein 1, particularly those presented in stage I and II of the tumor.…”

Section: Cancermentioning

confidence: 99%

Clinical implications of phage display technique: Digging deeper in diagnostic and therapeutic grounds.

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Phage display is considered as a gold-standard technique for the extraction of monoclonal antibodies. Within the past decade, the technique has been extensively applied in clinical studies for the discovery of peptides, ligands and antibodies that can be implemented for the detection of clinical biomarkers and therapeutic purposes. This review highlights recent advancements in the field of phage display technology that has aided medicine in therapeutic and diagnostic terms.

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Phage display-based on-slide selection of tumor-specific antibodies on formalin-fixed paraffin-embedded human tissue biopsies

Cited by 13 publications

References 50 publications

Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries

Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries

CSPG4: A Target for Selective Delivery of Human Cytolytic Fusion Proteins and TRAIL

Clinical implications of phage display technique: Digging deeper in diagnostic and therapeutic grounds.

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