Phage-displayed peptides that mimic aflatoxin B1 in serological reactivity

Abstract

Aims: To test phage-displayed random peptide libraries as sources of peptides that mimic the binding of a¯atoxin B1 to monoclonal antibodies raised against the toxin. Methods and Results: For two of the three MAbs tested, clones were obtained by panning, producing phage that bound speci®cally to MAb 13D1±1D9 (MAb 24; speci®c for a¯atoxins B1 and G1) and MAb 6E12±1E9 (MAb 13; speci®c for a¯atoxins B1, G1 and B2) in ELISA. The amino acid sequences of the binding peptides varied. Those binding to MAb 24 contained the sequence of`¼YMD¼', and those that bound to MAb 13 contained the dipeptide`PW'. Mimotope phage was used in a competition ELISA format for assaying a¯atoxin concentrations. Conclusions:The results show that mimotope preparations are effective substitutes for pure toxin in these ELISA procedures. Signi®cance and Impact of the Study: 1These results should contribute signi®cantly to enhancing the safety and diminishing the costs of a¯atoxin assays.

Development of a colloidal gold strip for rapid detection of ochratoxin A with mimotope peptide

“…At the same time, this reduces the potential exposure of the producer to OTA that might occur in the manufacture of the test strip. Following the successfully acquired mimotope peptides that mimic deoxynivalenol (Yuan et al, 1999) and aflatoxin B1 (Thirumala-Devi et al, 2001), mimotope peptides were used to set up a competitive ELISA for detecting OTA in our laboratory. Its linear range was 200-8000 pg/ml (Liu et al, 2007).…”

Development of Phage Immuno-Loop-Mediated Isothermal Amplification Assays for Organophosphorus Pesticides in Agro-products

“…26−28 Phage g3p-displayed short peptide libraries have been approved to be efficient tools for selecting mimotope peptides of an array of compounds, including metabolites of pyrethroid insecticides, 29 deoxynivalenol, 30 zearalenone, 31 ochratoxin A (OTA), 32 and aflatoxin. 33,34 The unique characteristics of a phage-borne peptide that connects the peptide with affinity to a target on the phage particles containing nucleic acids (single-stranded DNA) encoding the peptide make them excellent reagents to develop LAMP assays for small molecules.…”

An Immunoassay for Determining Aflatoxin B1 Using a Recombinant Phage as a Non-toxic Coating Conjugate

“…However, the immunoassay for AFB 1 involves using the mycotoxin in a conjugated form, which may be toxic to users and manufacturers. One approach to avoid using the toxic conjugated mycotoxin is to develop protein or peptide substitutes which mimic AFB 1 [8] [9] [10]. The phagedisplayed random peptide library, which displays extensive random peptides on the N terminus of the minor coat protein g p of the filamentous phage M13, can be used for this purpose.…”