Phages and Enzybiotics in Food Biopreservation

Ramos-Vivas, José; Zabaleta, María Eléxpuru; Sámano, María Luisa; Barrera, Alina Pascual; Forbes-Hernández, Tamara Y.; Giampieri, Francesca; Battino, Maurizio

doi:10.3390/molecules26175138

Cited by 40 publications

(10 citation statements)

References 235 publications

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“…An example of this would be the endolysins, a remarkably diverse group of catalytic enzymes that degrade the cell wall of the host so that the phage progeny can escape ( 26 ). In recent years, these proteins have awakened increased interest for their potential to be used as antimicrobial agents ( 27 , 28 ). Culture-free approaches have been applied to great effect in order to broaden the diversity of endolysins.…”

Section: Resultsmentioning

confidence: 99%

Long-Read Metagenomics Improves the Recovery of Viral Diversity from Complex Natural Marine Samples

et al. 2022

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We explored the vast genetic diversity of environmental viruses by using a combination of cellular metagenome (as opposed to virome) sequencing using high-fidelity long-read sequences (in this case, PacBio CCS). This approach resulted in the recovery of a representative sample of the viral population, and it performed better (more phage contigs, larger average contig size) than Illumina sequencing applied to the same sample.

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Section: Resultsmentioning

confidence: 99%

Long-Read Metagenomics Improves the Recovery of Viral Diversity from Complex Natural Marine Samples

et al. 2022

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“…There are several advantages to using phages over chemical antibacterial agents, including the fact that they do not contribute to deterioration of the product's quality; that they may be applied to a wide range of substrates; and that resistance to viruses can be addressed much more simply, in comparison to commonly used antibacterial agents [66][67][68]. It has been found that phages can naturally be found in food, which indicates that this is one way for them to come into contact with humans [69]. In addition to being cost-effective and easy to use, phage-based techniques are establishing themselves as an alternative to traditional antibacterial treatments.…”

Section: Introductionmentioning

confidence: 99%

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

et al. 2023

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Pathogens found in food are believed to be the leading cause of foodborne illnesses; and they are considered a serious problem with global ramifications. During the last few decades, a lot of attention has been paid to determining the microorganisms that cause foodborne illnesses and developing new methods to identify them. Foodborne pathogen identification technologies have evolved rapidly over the last few decades, with the newer technologies focusing on immunoassays, genome-wide approaches, biosensors, and mass spectrometry as the primary methods of identification. Bacteriophages (phages), probiotics and prebiotics were known to have the ability to combat bacterial diseases since the turn of the 20th century. A primary focus of phage use was the development of medical therapies; however, its use quickly expanded to other applications in biotechnology and industry. A similar argument can be made with regards to the food safety industry, as diseases directly endanger the health of customers. Recently, a lot of attention has been paid to bacteriophages, probiotics and prebiotics most likely due to the exhaustion of traditional antibiotics. Reviewing a variety of current quick identification techniques is the purpose of this study. Using these techniques, we are able to quickly identify foodborne pathogenic bacteria, which forms the basis for future research advances. A review of recent studies on the use of phages, probiotics and prebiotics as a means of combating significant foodborne diseases is also presented. Furthermore, we discussed the advantages of using phages as well as the challenges they face, especially given their prevalent application in food safety.

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“…Currently, this formulation is being investigated for safety and efficacy of a single intravenous dose of N-Rephasin ® SAL200 for persistent S. aureus bacteremia under phase IIa clinical study [ 243 ]. Considering the efficacy of phages and phage-derived lysins, the USFDA has approved the application of bacteriophage-based products as an eco-friendly approach for the control of food borne pathogens [ 240 , 244 ]. Further, phage-derived lytic proteins possess several advantages over antibiotics, namely: (a) rapid and extensive bactericidal action against the target pathogen, (b) lack of resistance development due to their specific action on conserved structural components of bacteria, (c) synergistic action with other lysins or antibiotics, and (d) their effect on phenotypically resistant persister cells growing on mucosal surfaces or in biofilms [ 245 ].…”

Section: Strategies Targeting Antimicrobial-resistant Bacteriamentioning

confidence: 99%

Progress in Alternative Strategies to Combat Antimicrobial Resistance: Focus on Antibiotics

et al. 2022

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Antibiotic resistance, and, in a broader perspective, antimicrobial resistance (AMR), continues to evolve and spread beyond all boundaries. As a result, infectious diseases have become more challenging or even impossible to treat, leading to an increase in morbidity and mortality. Despite the failure of conventional, traditional antimicrobial therapy, in the past two decades, no novel class of antibiotics has been introduced. Consequently, several novel alternative strategies to combat these (multi-) drug-resistant infectious microorganisms have been identified. The purpose of this review is to gather and consider the strategies that are being applied or proposed as potential alternatives to traditional antibiotics. These strategies include combination therapy, techniques that target the enzymes or proteins responsible for antimicrobial resistance, resistant bacteria, drug delivery systems, physicochemical methods, and unconventional techniques, including the CRISPR-Cas system. These alternative strategies may have the potential to change the treatment of multi-drug-resistant pathogens in human clinical settings.

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Phages and Enzybiotics in Food Biopreservation

Cited by 40 publications

References 235 publications

Long-Read Metagenomics Improves the Recovery of Viral Diversity from Complex Natural Marine Samples

Long-Read Metagenomics Improves the Recovery of Viral Diversity from Complex Natural Marine Samples

An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens

Progress in Alternative Strategies to Combat Antimicrobial Resistance: Focus on Antibiotics

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