Phagocytic activity and bactericidal capacity of polymorphonuclear leukocytes in children with recurrent otitis media

Lalchev, S.; Karchev, Todor

doi:10.1016/s0165-5876(85)80029-x

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…In contrast, rabbit neutrophils harvested from 7-day-old sterile wounds had increased 02 and hydrogen peroxide production after in vitro stimulation with phorbol myristate acetate, N-formyl-methionyl-leucyl-phenylalanine, and zymosan compared with that of blood neutrophils (26), perhaps reflecting the different chemotactic stimuli. Further study of exudate neutrophils, especially in humans, is necessary to complement the extensive literature that has documented altered blood neutrophil function and oxidative product formation during acute bacterial infections (6,9,15,19,20,30,31).…”

Section: Discussionmentioning

confidence: 99%

Oxidative metabolic products released from polymorphonuclear leukocytes in middle ear fluid during experimental pneumococcal otitis media

Kawana

Yokoo

et al. 1991

Infect Immun

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To determine whether oxidative metabolic products of phagocytic cells are present in the middle ear during experimental pneumococcal otitis media, we measured the concentration of myeloperoxidase (MPO) in middle ear fluid (MEF) and the capacity of neutrophils isolated from MEF and peripheral blood to produce MPO and superoxide anion (O2-) after in vitro stimulation. Free MPO in MEF was significantly increased 24 and 48 h after either viable or nonviable pneumococci were inoculated into the middle ear. In vitro-stimulated production of MPO and O2- from middle ear neutrophils was significantly less than that from peripheral blood neutrophils 24 h after nonviable pneumococci were inoculated but similar to it after 48 h. Twenty-four hours after viable pneumococci were inoculated, middle ear neutrophils stimulated in vitro produced less MPO but the same amount of O2- as did blood neutrophils. Oxidative metabolic products, therefore, are released from phagocytic cells into the MEF during pneumococcal otitis media, and future studies will need to define the contribution of these products to acute and chronic middle ear tissue injury.

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Section: Discussionmentioning

confidence: 99%

Oxidative metabolic products released from polymorphonuclear leukocytes in middle ear fluid during experimental pneumococcal otitis media

Kawana

Yokoo

et al. 1991

Infect Immun

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Preliminary Report on L-Forms: Possible Role in the Infectious Origin of Secretory Otitis Media

Ataoglu¹,

Göksu²,

Kemaloğlu³

et al. 1994

Ann Otol Rhinol Laryngol

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Infection and inflammation of the middle ear cleft are important factors in the pathogenesis of secretory otitis media. Although high percentages of negative cultures are confronted in many studies, strong evidence pointing to the infectious nature of this disease could not be overlooked. Many authors agree about the failure of conventional culture methods in identifying the responsible pathogen or pathogens. Besides, some agents, such as some kinds of antibiotics, lysozyme, and perhaps some undetected materials, are capable of changing bacterial behavior and consequently the clinical course. Effusions taken from 40 ears with secretory otitis media were cultured by means of conventional brain-heart infusion broth and special hypertonic thioglycollate broth. Strikingly, bacterial L-forms were detected in 6 specimens in thioglycollate broth, with no growth in the conventional broth. We concluded that these atypical forms of bacteria, the L-forms, may play an important role in the bacteriologic aspect of secretory otitis media.

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Phagocytic activity and bactericidal capacity of polymorphonuclear leukocytes in children with recurrent otitis media

Cited by 2 publications

References 11 publications

Oxidative metabolic products released from polymorphonuclear leukocytes in middle ear fluid during experimental pneumococcal otitis media

Oxidative metabolic products released from polymorphonuclear leukocytes in middle ear fluid during experimental pneumococcal otitis media

Preliminary Report on L-Forms: Possible Role in the Infectious Origin of Secretory Otitis Media

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