Phagocytic activity of neutrophils and lymphocytes at various periods of storage in the state of cold anabiosis (-40�C)

Lymphocyte resistance to cold anabiosis of different degree

“…However, the resistance to physicochemical factors during different stages of cryopreservation at ultralow temperature (-196 o C) is different in T and B lymphocytes. For example, T lymphocytes are more sensitive to osmotic shock than B lymphocytes.We previously studied the function of granulocytes at submoderately low (-20 o C) [4,5], moderately low (-40 o C) [3,7], and low (80 o C) [6] temperatures, while differentiated evaluation of T and B lymphocytes was never carried out.We studied the resistance of T and B lymphocytes to cold anabiosis of different depth and duration. …”

“…We previously studied the function of granulocytes at submoderately low (-20 o C) [4,5], moderately low (-40 o C) [3,7], and low (80 o C) [6] temperatures, while differentiated evaluation of T and B lymphocytes was never carried out.…”