Phagocytic function of Kupffer cells in mouse nonalcoholic fatty liver disease models: Evaluation with superparamagnetic iron oxide

Abstract

KC phagocytic dysfunction is aggravated in the progression of NAFLD and may be reversible with therapeutic intervention. SPIO-MRI may be useful for classifying the severity of NAFLD and monitoring the treatment response of NAFLD.

Kinetic analysis of superparamagnetic iron oxide nanoparticles in the liver of body-temperature-controlled mice using dynamic susceptibility contrast magnetic resonance imaging and an empirical mathematical model

“…Our results (Figs. [3][4][5][6][7][8] suggest that our method is useful for analyzing the kinetic behavior of SPIONs in the liver and for quantifying the responses of Kupffer cells to various drugs.…”

“…Recently, Tsujimoto et al [7] investigated the phagocytic activity of Kupffer cells in a rat NASH model using contrast-enhanced ultrasonography and reported reduced phagocytic function of Kupffer cells in the rat NASH model. Several studies have investigated the phagocytic function of Kupffer cells using dynamic susceptibility contrast MRI (DSC-MRI) with SPIONs in animal models and patients with NAFLD or NASH [8,9]. In these studies, however, semi-quantitative indices such as a relative signal intensity loss [8] and liver-to-muscle signal intensity ratio [9] have been used to analyze the function of Kupffer cells.…”

“…Several studies have investigated the phagocytic function of Kupffer cells using dynamic susceptibility contrast MRI (DSC-MRI) with SPIONs in animal models and patients with NAFLD or NASH [8,9]. In these studies, however, semi-quantitative indices such as a relative signal intensity loss [8] and liver-to-muscle signal intensity ratio [9] have been used to analyze the function of Kupffer cells. To the best of our knowledge, no report has studied in detail the kinetic behavior of SPIONs in the liver.…”

Evaluation of non-targeting, C- or N-pH (low) insertion peptide modified superparamagnetic iron oxide nanoclusters for selective MRI of liver tumors and their potential toxicity in cirrhosis

“…The enhancement by SPIONs in cirrhosis had been attributed to that cirrhosis only resulted in partial dysfunctional Kupffer cells that were still capable to take up SPION. [9][10][11][12][13]…”

“…[5][6][7][8] In addition, SPIO has also been used to assess stages of cirrhosis and non-alcoholic steatohepatitis as well as the effectiveness of treatment of non-alcoholic fatty liver disease. [9][10][11][12][13] Furthermore, SPIO has been widely used in diagnosis and therapy of other cancers such as prostate cancer diagnosis and drug delivery. [14][15][16][17][18][19] On the other hand, the potential cytotoxicity of SPIO has recently been considered as a major safety concern, [19][20][21] especially in chronic liver diseases due to SPIO induced iron overload resulting in increased risks of steatohepatitis and cirrhosis progression and liver lesions in obesity.…”