Phagocytosis and intracellular killing of pathogenic yeasts by human monocytes and neutrophils

Schuit, Kenneth E.

doi:10.1128/iai.24.3.932-938.1979

Cited by 68 publications

(20 citation statements)

References 18 publications

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“…[12], whereas Rodey et al [15] found equal functional ciipacitics LHHI Scluiit [16] an even more aciive bchi.i\iour of monocytes than granulocytes in the phagocytosis of Candida species. So far.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Phagocytosis and Intracellular Killing of Staphytococcus aureus and Escherichia coli by Human Monocytes

1981

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The kinetic patterns of the phagocytosis and intracellular killing of Staphylococcus and Escherichia coli by monocytes were investigated separately to acquire more insight into the total process, i.e. from the ingestion to the death of the micro-organisms. Phagocytosis proved to be dependent on: (1) both the bacteria-to-monocyte ratio and the monocyte concentration; a concentration of at least 5 x 10(5) monocytes/ml proved necessary for the measurement of ingestion, whereas the rate of ingestion was found to be proportional to the number of extracellular bacteria until a maximum rate is reached, (2) the serum concentration in the incubation medium, which influenced both the rate of phagocytosis and the maximum number of bacteria taken up by one monocyte, and (3) the temperature, the highest rate of phagocytosis being reached at 37-41 degrees C. The intracellular killing proved to be dependent on: (1) the number of bacteria ingested; the rate of killing was proportional to the number of ingested bacteria until a maximum rate was reached; (2) the temperature, since a maximum rate of killing is only reached at 37-41 degrees C; at lower and higher temperatures the rate of killing is lower, in the latter case due to inactivation of extracellular stimuli. These separate data on the ingestion and killing processes made it possible to compute the theoretical numbers of extracellular, viable intracellular, and total intracellular bacteria for a model system consisting of 5 x 10(6) monocytes, 5 x 10(6) bacteria, and 10% serum. These calculated values are in agreement with the experimental data.

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Section: Discussionmentioning

confidence: 99%

Kinetics of Phagocytosis and Intracellular Killing of Staphytococcus aureus and Escherichia coli by Human Monocytes

1981

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“…albicans killing assay. Phagocytosis and intracellular killing of C. albicans by PM4 and monocytes were assessed by a previously described fluorochrome (acridine orange) microassay (44) modified for the purposes of this study. A clinical isolate of C. albicans was cultured for 18 h at 37°C in Sabouraud 2% dextrose broth (Difco Laboratories, Detroit, Mich.).…”

Section: Methodsmentioning

confidence: 99%

“…A mixture of opsonized C. albicans (2 x 106 blastospores in 100 ,u of GHBSS) and phagocytes (5 x 105 cells in 100 ,u of GHBSS) was incubated for 30 min at 37°C in a shaking incubator (250 rpm). After the pellets were washed and resuspended in 1.0 ml of GHBSS, 200-pAl portions of the final suspensions were deposited onto glass cover slips, incubated for 60 min at 37°C, and then stained with acridine orange (44). Preparations were examined under a 100x oil immersion objective with a UV fluorescence microscope (Leitz, Wetzlar, Federal Republic of Germany).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial activities of dialysate-elicited and resident human peritoneal macrophages

Peterson¹,

Gaziano²,

Suh³

et al. 1985

Infect Immun

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Recent studies of the antimicrobial capacity of peritoneal macrophages (PM4+) isolated from patients undergoing chronic peritoneal dialysis have raised the question of whether these cells might be analogous to stimulated or activated murine PM+. To explore this possibility, we compared PM+ from these patients (dialysate-elicited PM+) with PM4 obtained from women undergoing laparoscopy (resident PM4) in several in vitro assays of phagocyte function. Although bacterial phagocytosis by cells from both groups of donors was similar, significant differences were found in their chemiluminescence responses to opsonized zymosan.Although the mean peak luminol-enhanced chemiluminescence response of dialysate-elicited PM+ was 4.7 x 105 cpm, that of resident PM4 was only 1.3 x 105 cpm (P < 0.05). In a lucigenin-enhanced chemiluminescence assay, dialysate-elicited PM4 again generated significantly greater chemiluminescence than did resident PM4, 212 on July 4, 2020 by guest http://iai.asm.org/ Downloaded from Immun. 24:932-938. 45. Seim, S. 1983. Role of myeloperoxidase in the luminol-dependent chemiluminescence response of phagocytosing human VOL. 49, 1985 on July 4, 2020 by guest http://iai.asm.org/ Downloaded from

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“…5 ml saline, and the suspension was cultured on a standard Mullar Hinton agar plate. After 24 h incubation at 37ЊC, the number of colonies was counted to determine the number of viable leucocyte-associated bacteria [12][13][14].…”

Section: Activity Of Alveolar Macrophagesmentioning

confidence: 99%

Protective effect of granulocyte colony-stimulating factor (G-CSF) in a granulocytopenic mouse model of Pseudomonas aeruginosa lung infection through enhanced phagocytosis and killing by alveolar macrophages through priming tumour necrosis factor-alpha (TNF-α) production

Shirai

Kadota

Tomono

et al. 1997

Clinical and Experimental Immunology

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SUMMARYWe investigated the effects of G-CSF in a granulocytopenic mouse model of Pseudomonas aeruginosa lung infection. The model was prepared by intratracheal instillation of the bacteria, while granulocytopenia was induced by intraperitoneal injection of 4 . 0 mg of cyclophosphamide (CPA). There was no difference in the survival rate between G-CSF-treated animals and the normal group, and the number of neutrophils in the blood and lung recovered to normal in the former group. However, the phagocytic and killing activities of neutrophils were lower in G-CSF-treated mice than in controls. Interestingly, the mortality rate increased significantly when anti-TNF-a antibody was combined with G-CSF, although it was intermediate between CPA alone and CPA-G-CSF-treated mice. However, the improved mortality was not associated with a change in the number of neutrophils in the circulation and lung. Administration of anti-TNF-a antibody resulted in a significant suppression of TNF-a in bronchoalveolar lavage fluid and of enhanced alveolar macrophage function (phagocytic and bactericidal activity) against P. aeruginosa in G-CSF-treated granulocytopenic mice. We showed also increased TNF-a mRNA expression and TNF-a production in vitro using G-CSF-pretreated alveolar macrophages compared with control untreated macrophages. Our results are the first evidence to suggest that G-CSF provides a synergistic protective effect against lethal P. aeruginosa lung infection in the granulocytopenic host. This effect is probably due to enhancement of alveolar macrophage function through endogenous TNF-a production, in addition to increasing the number of circulating neutrophils.

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Phagocytosis and intracellular killing of pathogenic yeasts by human monocytes and neutrophils

Cited by 68 publications

References 18 publications

Kinetics of Phagocytosis and Intracellular Killing of Staphytococcus aureus and Escherichia coli by Human Monocytes

Kinetics of Phagocytosis and Intracellular Killing of Staphytococcus aureus and Escherichia coli by Human Monocytes

Antimicrobial activities of dialysate-elicited and resident human peritoneal macrophages

Protective effect of granulocyte colony-stimulating factor (G-CSF) in a granulocytopenic mouse model of Pseudomonas aeruginosa lung infection through enhanced phagocytosis and killing by alveolar macrophages through priming tumour necrosis factor-alpha (TNF-α) production

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