Phagocytosis of enteric<i>Campylobacter</i>by human and murine granulocytes

Bär, Werner; Glenn-Calvo, Eduardo; Krausse, Rea

doi:10.1111/j.1574-6968.1991.tb04207.x

Cited by 4 publications

(2 citation statements)

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“…Furthermore we showed that although neutrophils respond towards C. concisus without these being opsonised with serum the response is increased when bacteria are opsonised. Previous studies have found that opsonised C. jejuni generates a higher degree of neutrophil granulocyte response than non-opsonised bacteria [1,2,15] correlating well with our findings. Our study design does not allow us to definitely conclude whether opsonisation induces increased phagocytosis of C. concisus .…”

Section: Discussionsupporting

confidence: 92%

“…Our study design does not allow us to definitely conclude whether opsonisation induces increased phagocytosis of C. concisus . Previous investigations of C. jejuni and H. pylori [2-4] have shown that opsonisation significantly increases phagocytosis in both complement-dependent and –independent assays. Combination of heat-treated and untreated serum produced a higher opsonic activity for C. jejuni stimulation of neutrophils [4].…”

Section: Discussionmentioning

confidence: 99%

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Neutrophil activation by Campylobacter concisus

et al. 2013

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BackgroundCampylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. The magnitude of systemic immunoglobulin response in acute infection is unknown.MethodsNeutrophils from healthy volunteers were activated with five faecal isolates of C. concisus from patients with gastroenteritis as well as the oral reference strain C. concisus ATCC33237. Neutrophils were tested for the expression of adherence molecule CD11b by immunoflourescence and for oxidative burst response by chemiluminescence. The opsonic activity in a chemiluminescence assay was assessed with heat treated serum from patients with C. concisus infection.ResultsA strong and dose-dependent activation of neutrophil adherence molecule CD11b and oxidative burst response was demonstrated with all six C. concisus isolates. Bacteria opsonised in heat treated serum induced an increased chemiluminescence response. Heat treated serum from patients with C. concisus infection did not have a higher opsonic activity than heat treated serum from healthy volunteers.ConclusionC. concisus has the capability to activate the innate immune system by stimulating neutrophil cells to increased adherence molecule expression and oxidative burst response, both crucial for acute inflammation. In a chemiluminescence assay the opsonic activity of heat treated serum from patients was not increased compared to heat treated control serum suggesting a weak systemic IgG response to infection.

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Section: Discussionsupporting

confidence: 92%