Pharmaceutical Applications of Hydrophilic Interaction Chromatography

Olsen, Bernard A.; Risley, Donald S.; Sharp, V. Scott; Pack, Brian W.; Lytle, Michelle L.

doi:10.1002/9781118495247.ch4

Cited by 2 publications

(1 citation statement)

References 83 publications

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“…HILIC applications are most frequent in biological , pharmaceutical , and metabolite analysis. An excellent book on HILIC has appeared recently, covering various aspects of the technique: separation mechanism, stationary phases, method development, and applications .…”

Section: Introductionmentioning

confidence: 99%

Mobile phase effects on the retention on polar columns with special attention to the dual hydrophilic interaction–reversed‐phase liquid chromatography mechanism, a review

Jandera

Hájek

2017

J of Separation Science

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Hydrophilic interaction liquid chromatography on polar columns in aqueous-organic mobile phases has become increasingly popular for the separation of many biologically important compounds in chemical, environmental, food, toxicological, and other samples. In spite of many new applications appearing in literature, the retention mechanism is still controversial. This review addresses recent progress in understanding of the retention models in hydrophilic interaction liquid chromatography. The main attention is focused on the role of water, both adsorbed by the column and contained in the bulk mobile phase. Further, the theoretical retention models in the isocratic and gradient elution modes are discussed. The dual hydrophilic interaction liquid chromatography reversed-phase retention mechanism on polar columns is treated in detail, especially with respect to the practical use in one-and two-dimensional liquid chromatography separations.

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Section: Introductionmentioning

confidence: 99%

Mobile phase effects on the retention on polar columns with special attention to the dual hydrophilic interaction–reversed‐phase liquid chromatography mechanism, a review

Jandera

Hájek

2017

J of Separation Science

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Metabolomics in clinical and forensic toxicology, sports anti‐doping and veterinary residues

Keen

Cawley

Reedy

et al. 2022

Drug Testing and Analysis

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Metabolomics is a multidisciplinary field providing workflows for complementary approaches to conventional analytical determinations. It allows for the study of metabolically related groups of compounds or even the study of novel pathways within the biological system. The procedural stages of metabolomics; experimental design, sample preparation, analytical determinations, data processing and statistical analysis, compound identification and validation strategies are explored in this review. The selected approach will depend on the type of study being conducted. Experimental design influences the whole metabolomics workflow and thus needs to be properly assessed to ensure sufficient sample size, minimal introduced and biological variation and appropriate statistical power. Sample preparation needs to be simple, yet potentially global in order to detect as many compounds as possible. Analytical determinations need to be optimised either for the list of targeted compounds or a universal approach. Data processing and statistical analysis approaches vary widely and need to be better harmonised for review and interpretation. This includes validation strategies that are currently deficient in many presented workflows. Common compound identification approaches have been explored in this review. Metabolomics applications are discussed for clinical and forensic toxicology, human and equine sports anti‐doping and veterinary residues.

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Pharmaceutical Applications of Hydrophilic Interaction Chromatography

Cited by 2 publications

References 83 publications

Mobile phase effects on the retention on polar columns with special attention to the dual hydrophilic interaction–reversed‐phase liquid chromatography mechanism, a review

Mobile phase effects on the retention on polar columns with special attention to the dual hydrophilic interaction–reversed‐phase liquid chromatography mechanism, a review

Metabolomics in clinical and forensic toxicology, sports anti‐doping and veterinary residues

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