Pharmaceutical suspension containing both immediate/sustained-release amoxicillin-loaded gelatin nanoparticles: preparation and in vitro characterization

Abstract

Pharmaceutical suspension containing oral dosage forms delivering both immediate-release and sustained-release amoxicillin was developed as a new dosage form to eradicate Helicobacter pylori. Amoxicillin-loaded gelatin nanoparticles are able to bind with the mucosal membrane after delivery to the stomach and could escalate the effectiveness of a drug, providing dual release. The objective of this study was to develop amoxicillin nanoparticles using innovative new technology – the Büchi Nano Spray Dryer B-90 – and investigate such features as drug content, particle morphology, yield, in vitro release, flow properties, and stability. The nanoparticles had an average particle size of 571 nm. The drug content and percentage yield was 89.2% ± 0.5% and 93.3% ± 0.6%, respectively. Angle of repose of nanoparticle suspension was 26.3° and bulk density was 0.59 g/cm3. In vitro drug release of formulations was best fitted by first-order and Peppas models with R2 of 0.9841 and 0.9837 respectively; release profile was 15.9%, while; for the original drug, amoxicillin, under the same conditions, 90% was released in the first 30 minutes. The nanoparticles used in this study enabled sustained release of amoxicillin over an extended period of time, up to 12 hours, and were stable for 12 months under accelerated storage conditions of 25°C ± 2°C and 60% ± 5% relative humidity.

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

“…In this study, the SLNs produced in a single batch were weighed, and the percentage yield was calculated by using the following formula: 19 Percentage yield Actual weight of nanoparticles produced Th = e eoretical weight of nanoparticles produced…”

In Vitro and In Vivo Evaluation of Nanoparticles Prepared by Nano Spray Drying for Stomach Mucoadhesive Drug Delivery

“…A dry syrup was prepared by adding 10 mL of distilled water with stirring until a uniform/homogenous mixture was obtained. [31,54,55] …”

“…Additionally, nanoparticles also improve the bioavailability of drugs and protect against the drug degradation that occurs in response to physiological barriers. [23,24] Several approaches have been anticipated to retain the formulations in the stomach; these include mucoadhesive microspheres, [25] hydrogel nanoparticles, [26] beta-cyclodextrin microspheres, [27] gellan beads, [28] floating microballoons, [29] spray-dried nanoparticles, [30,31] and so on, which were able to stay in the GIT for a longer period of time. However, when compared to microspheres/beads, the nanoparticles (due to their size) can cover a larger surface area, and they exhibit an increase in mucin adsorption, which leads to great improvements in the mucoadhesive properties of the nanoparticles.…”

“…[34] Spray-drying is a well-recognized single step; it is rapid and is frequently used in a variety of drug delivery sciences to produce a dry powder from a liquid feed. [30,31,[35][36][37] These techniques are not still ideal, and many aspects need to be revised. First, the drug loading and encapsulation efficiency into the particles are not high.…”

Bovine serum albumin nanoparticles for delivery of tacrolimus to reduce its kidney uptake and functional nephrotoxicity

“…Nanoparticles are made of a variety of polymers, such as polysaccharides (Fernandez-Urrusuno et al, 1999;Liu et al, 2008), proteins (Elzoghby, 2013;Harsha, 2013) and synthetic polymers (Breunig et al, 2008;Fattal et al, 1998). Among all the available materials, the versatile albumin is an ideal material to fabricate nanoparticles for drug delivery due to its nontoxic, nonimmunogenic, biocompatible and biodegradable properties (Kratz, 2008).…”