Pharmacognostic and Phytochemical Studies of &amp;lt;i&amp;gt;Smilax domingensis&amp;lt;/i&amp;gt; Willd. in Cuba

Yaque, José González; Monan, Max; Cuéllar, Armando Cuéllar; Armas, Teylor de; Gómez, Enrique; Dopico, Eniel

doi:10.4236/ajps.2017.86100

Cited by 5 publications

(5 citation statements)

References 5 publications

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“…The presence of parenchyma tissue, xylematic vessels, fibers and starch granules are reported in S. domingensis from Cuba and Guatemala (Cáceres et al, 2012;González et al, 2017), which was supported by the present study. The difference lies within the morphology and arrangement of these structures.…”

Section: Microscopic Analysissupporting

confidence: 90%

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

Soledispa

Santos-Ordóñez

Miranda

et al. 2021

PeerJ

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Smilax plants are distributed in tropical, subtropical, and temperate regions in both hemispheres of the world. They are used extensively in traditional medicines in a number of countries. However, morphological and molecular barcodes analysis, which may assist in the taxonomic identification of species, are lacking in Ecuador. In order to evaluate the micromorphological characteristics of these plants, cross sections of Smilax purhampuy leaves were obtained manually. The rhizome powder, which is typically used in traditional medicines, was analyzed for micromorphological characteristics. All samples were clarified with 1% sodium hypochlorite. Tissues were colored with 1% safranin in water and were fixed with glycerinated gelatin. DNA was extracted from the leaves using a modified CTAB method for molecular barcode characterization and PCR was performed using primers to amplify the different loci including the plastid genome regions atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer; and the nuclear DNA sequence ITS2. A DNA sequence similarity search was performed using BLAST in the GenBank nr database and phylogenetic analysis was performed using the maximum likelihood method according to the best model identified by MEGAX using a bootstrap test with 1,000 replicates. Results showed that the micromorphological evaluation of a leaf cross section depicted a concave arrangement of the central vein, which was more pronounced in the lower section and had a slight protuberance. The micromorphological analysis of the rhizome powder allowed the visualization of a group of cells with variable sizes in the parenchyma and revealed thickened xylematic vessels associated with other elements of the vascular system. Specific amplicons were detected in DNA barcoding for all the barcodes tested except for the trnH–psbA spacer. BLAST analysis revealed that the Smilax species was predominant in all the samples for each barcode; therefore, the genus Smilax was confirmed through DNA barcode analysis. The barcode sequences psbK-psbI, atpF-atpH, and ITS2 had a better resolution at the species level in phylogenetic analysis than the other barcodes we tested.

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Section: Microscopic Analysissupporting

confidence: 90%

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

Soledispa

Santos-Ordóñez

Miranda

et al. 2021

PeerJ

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“…TLC results evidenced the presence of chemical compounds type flavonoids, some structures related with terpenoids structures and the presence of phenolic compounds derives from catechol (Yaque et al, 2017). Further studies are needed to establish the molecules responsible for the chemical composition and the biological activities attributed to this rhizome, specially using HPLC-MS or LC-NMR.…”

Section: Journal Of Agricultural Studiesmentioning

confidence: 98%

“…Preliminary phytochemical screening suggested the presence of flavonoids, alkaloids, coumarins, catechins, pirochatecolic tannins, fat and/or volatile oils, saponins, triterpens and/or steroid, quinones and reducing sugars, and the absence of resins, amino acids or amines, cardiotonic glycosides, anthocyanidins and astringents and/or bitter principles Data referred here refer to evaluations with wild material in our country (Yaque et al, 2017).…”

Section: Chemical Characterization By Gc-msmentioning

confidence: 99%

GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

Soledispa¹,

González²,

Cuéllar³

et al. 2018

JAS

Self Cite

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A preliminary chemical characterization of main components of ethanolic extract with dried rhizomes of Smilax domingensis Wid. that grow in Cuba was done using a GCMS-QP2010 Ultra Shimadzu and the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. After sample derivatization 125 chemical compounds were registered by the equipment and from them, 35 different chemical components were characterized and reported for the first time from this part of the plant in our country. The results demonstrate the developed method could be employed as a rapid and versatile analytical technique for identification of chemical constituents and quality control of Smilax domingensis.

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“…So it is very essential to analyse pharmacognostic study in plants [8]. Some of the examples for pharmacognostic studies are Tephrosia purpurea root [9]; Ferulasumbul root [10]; rhizome of Smilax domingensis [11]; Mangiferaindica leaf [12]; Diplaziumesculentum leaf [13]; Cissusquadrangularis stem [14]; Argyreiapilosa stem [15]; fruit of Helicteresisora [16]; flowers of Woodfordiafruticosa [17] and Aervalanata [18].The antimicrobial action of plant on microorganisms are due to the presence of certain antimicrobial agents such as alkaloids, flavonoids, volatile oils, gums, tannins, saponins, steroids and some other secondary metabolites present in the plants [19][20][21].Therefore, the studyobjective was to assess pharmacological potentials like phytochemical constituents, antioxidant and antibacterial of the crude and solvent extracts of H.alternataleaves.…”

Section: Introductionmentioning

confidence: 99%

Examination of Phytochemicals, Antioxidant and Antibacterial Activity of Hemigraphisalternate

Agneeswari,

Jansi

2019

IJEAT

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Plants as the source of medicine plays avital role in the health service around world. Hemigraphis alternata (Acanthaceae), exotic plants adapt to India, is versatile tropical lower-creeping perennial herbs that reachheight of 15 - 30 centimeters. The matured leaf of Hemigraphis alternata was collected from Kurunthankode , Kanyakumari district. Phytochemical screening of the H.alternata revealed the presence of some phytoconstituents such as carbohydrate, protein, alkaloid, flavonoid, saponin, terpenoid and tannin. Previous report shows that H.coloratacontains similar compounds like saponins, flavonoids and terpenoids. The antioxidant property of H.alternata extract were compared with standard ascorbic acid. The ethanolic extract of H.alternata leaf exhibit good scavenging activity with dose dependent manner. Antimicrobial activities of H.alternata leaf extract show the ethanol extract has the maximum activity against bacteria and fungi. This study revealed the presence of different Phytochemical, antioxidant and antibacterial activities of plant H.alternataand can be suggested that the bioactive components are promising natural antimicrobial agents.

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Pharmacognostic and Phytochemical Studies of <i>Smilax domingensis</i> Willd. in Cuba

Cited by 5 publications

References 5 publications

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

GC-MS Chemical Characterization of Main Components of Smilax Domingensis Wild. in Cuba

Examination of Phytochemicals, Antioxidant and Antibacterial Activity of Hemigraphisalternate

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