Pharmacognostic characterization and development of standardization parameters for the quality control of Entada africana Guill. &amp; Perr

Baidoo, Michael Frimpong; Asante-Kwatia, Evelyn; Mensah, Abraham Yeboah; Sam, George Henry; Amponsah, Isaac Kingsley

doi:10.1016/j.jarmap.2018.11.003

Cited by 18 publications

(27 citation statements)

References 14 publications

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“…E. africana is widely used to treat various ailments, among which liver related diseases [ 3 , 38 ]. Investigations from our research group have led to the isolation of an active fraction, namely EaF10 (fraction methylene chloride/methanol 90:10, v/v) with promising activity against toxic hepatitis [ 9 , 10 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of CYP2E1 and activation of Nrf2 signaling pathways by a fraction from Entada africana alleviate carbon tetrachloride-induced hepatotoxicity

Kouam

Owona

Fifen

et al. 2020

Heliyon

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Entada africana is used in non-conventional medicine for the management of liver ailments. A fraction, designated EaF10 (methylene chloride/methanol 90:10, v/v) with promising hepatoprotective activity has been isolated. Since the mechanisms underlying EaF10 hepatoprotective action remain unknown, this study was undertaken to investigate the anti-hepatotoxic mechanism of the fraction against carbon tetrachloride (CCl 4 )-induced hepatotoxicity and its antioxidant properties. Antioxidant activities of EaF10 were assessed through four chemical antioxidant assays and its anti-hepatotoxic effect evaluated in vivo and in vitro by post-treatment (25 or 100 mg/Kg) or co-treatment (6.25–100 μg/mL) in CCl 4 -intoxicated mice and normal human liver cells line L-02 hepatocytes respectively; and biochemical and molecular parameters assessed respectively by spectrophotometry, and by quantitative real-time polymerase chain reaction and western blot analysis. EaF10 exhibited strong antioxidant activities correlated with its polyphenol content. Serum levels of alanine/aspartate aminotransferase (AST/ALT) and nitrite oxide, liver contents of glutathione (GSH) protein carbonylation and malondialdehyde (MDA), liver activities of catalase (CAT), glutathione-S-transferase (GST) and superoxide dismutase (SOD) and cell viability showed the anti-hepatotoxic effect of EaF10, supported by histopathological observations. The fraction decreased the protein level of Cytochrome P450 2E1 (CYP2E1) and Kelch-like ECH-associated protein-1 (Keap-1), induced nuclear translocation of Nuclear factor-erythroid 2-related factor-2 (Nrf2) coupled to an increase of the mRNA levels of CAT, SOD1 and GST in CCl 4 -intoxicated L-02 hepatocytes. These findings evidenced that the studied plant fraction possesses a strong antioxidant capacity and prevents CCl 4 -induced hepatotoxicity, likely through inhibition of CYP2E1 and activation of the Nrf2 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Inhibition of CYP2E1 and activation of Nrf2 signaling pathways by a fraction from Entada africana alleviate carbon tetrachloride-induced hepatotoxicity

Kouam

Owona

Fifen

et al. 2020

Heliyon

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“…Similar phytochemical screening of the leaf and stem bark extract of Ea revealed the presence of glycosides, saponins, tannins, flavonoids, coumarins, triterpenes and sterols (Baidoo et al., 2018).…”

Section: Main Textmentioning

confidence: 99%

“…Microcopy of the whole leaf of Ea revealed wavy and straight walled epidermal cells, and the presence of hypostomatic paracytic stomata with an arch-shaped collaterally closed vascular bundle, collenchyma, sclerenchyma and palisade cells was reported. Microscopy of the powdered samples also revealed prismatic calcium oxalate crystals with stone cells, fibres, pitted and scalar form xylem vessels (Baidoo et al., 2018).…”

Section: Main Textmentioning

confidence: 99%

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The phytochemical and pharmacological actions of Entada africana Guill. & Perr.

Yusuf

Abdullahi

2019

Heliyon

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Entada africana (Ea, Asteraceae) has been widely used traditionally to treat different ailments, as food, insecticides, source of gums, in some small carpentry works. Studies on the pharmacognostic characterization, nutritional, elemental and physicochemical contents has been reported. In vivo and in vitro studies on the plant validated some ethnomedicinal claims of the use of the plant as an anti-inflammatory, analgesic, antibacterial, antioxidant, antiviral, anti-angiogenic, cytotoxic agents among others. Triterpenes, saponins, flavonoids and sugars were reported as bioactive constituents which might be responsible for the aforementioned pharmacological actions of the plant.However, more researches are required in order to isolate bioactive compounds from the different parts of Ea and evaluate their effects on different ailments.

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“…However, the major drawback to the use of herbal medicine is the lack of standardization. This paves way for wrong identification, unintended substitution of closely related species and intended adulteration of genuine drugs with quality less ones to meet the increasing demands of the herbal drugs [2]. Furthermore, herbal drugs are likely to decomposition, contamination and variation in composition of constituents, which gives rise to little or no therapeutic efficacy [3].…”

Section: Introductionmentioning

confidence: 99%

Morphoanatomical Studies of Tiliacora racemosa Colebr.

Yogeshwari¹

2020

azb

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Tiliacora racemosa is a climbing shrub belonging to the family Menispermaceae and is used in traditional medicine for treating different ailments such as snakebites, cuts and wounds, strangury and also used as a diuretic. The morphoanatomical studies help to identify diagnostic features for the identification and standardization of the medicinal plant. The plant material was fixed, sectioned using rotary microtome and stained with toluidine blue. The leaf consists of very thick midrib with small lateral veins both being plano-convex. The midrib consists of three top or egg shaped collateral vascular bundles which are surrounded by a common sclerenchymatous bundle sheath and the lamina is dorsiventral. The adaxial epidermis is apostomatic and the abaxial epidermis is stomatiferous which is of cyclocytic type. The stem is circular in sectional view and consists of about 19 discrete wedge shaped vascular bundles. The narrow and wide fibres, vessel elements were seen in powder microscopy. Wide fibres, vessel elements were seen in powder microscopy.

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Pharmacognostic characterization and development of standardization parameters for the quality control of Entada africana Guill. & Perr

Cited by 18 publications

References 14 publications

Inhibition of CYP2E1 and activation of Nrf2 signaling pathways by a fraction from Entada africana alleviate carbon tetrachloride-induced hepatotoxicity

Inhibition of CYP2E1 and activation of Nrf2 signaling pathways by a fraction from Entada africana alleviate carbon tetrachloride-induced hepatotoxicity

The phytochemical and pharmacological actions of Entada africana Guill. & Perr.

Morphoanatomical Studies of Tiliacora racemosa Colebr.

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