Pharmacognostical Screening and Determination of Antioxidant Activity of Nelumbo Nucifera Gaertn Ethanol Seed Extract by Different in Vitro Models

Bhardwaj, Alok; Modi, Ketan

doi:10.22159/ijpps.2017v9i3.16362

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…The mobile phase is a mixture of the solvent (all the solvents were distilled before being used) with certain ratio, that the obtained gradient eluent system with different polarity. Elution process using solvent mixture of low to high polarity (n-hexane -ethyl acetate -methanol) The fractions have been obtained is then evaporated and the chromatogram profiles identified by the method of TLC using silica gel 60 F 254 (Merck, 0,25 mm, normal phase) [13,14]. So that the fraction that has the same of chromatogram profile, then combined and conducted of testing the inhibitory activity of the α-glucosidase enzyme.…”

Section: Fractionation Processingmentioning

confidence: 99%

COMPARISON OF INHIBITORY ACTIVITY AGAINST THE Α-Glucosidase ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

Martha

Elya

Hanafi

2017

Asian J Pharm Clin Res

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Objective: Garcinia kydia Roxb. is a species of the genus Garcinia, is based chemotaxonomic has various bioactive compounds that have been isolated by a variety of pharmacological activities, one of the activities that are being developed that inhibition of α-glucosidase. However, α-glucosidase inhibitory activity in the extracts and fraction from leaves of the G. kydia Roxb. has not been reported. In this study, seeks to evaluate of α-glucosidase inhibitory activity against extracts and fractions of potentially. Methods:The α-glucosidase inhibitory activity test conducted by in vitro using the enzymatic reaction is measured of quantity with a microplate reader and identifies the compound from the active fraction with normal-phase thin layer chromatography. Results:The ethyl acetate and methanol extract have the potential to inhibit the α-glucosidase with the percent inhibition at a concentration of 500 µg/ml of 83% and 59%, respectively. The active fraction of the ethyl acetate extracts (FEA8) with percentage inhibition at concentrations of 100 µg/ml and inhibitory concentration (IC 50 ) values of 80% and 2.79 μg/ml, respectively, and active fraction of the methanol extracts (FMT3) with percent inhibition at concentrations of 100 µg/ml and IC 50 values of 71% and 8.43 μg/ml, respectively. Conclusion: G. kydiaRoxb. evident has the potential to inhibit the α-glucosidase. Flavonoid and phenolic compounds that suspected of acts as α-glucosidase inhibitory activity. Thus, the research will continue the process of isolating the active compound so that it can be developed as natural therapeutic agents in the control of glucose.

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Section: Fractionation Processingmentioning

confidence: 99%

COMPARISON OF INHIBITORY ACTIVITY AGAINST THE Α-Glucosidase ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

Martha

Elya

Hanafi

2017

Asian J Pharm Clin Res

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“…The disc diffusion technique was followed [24,27,30] at a concentration of 250 μg mL-1 ampicillin and streptomycin used as the standard drugs. The measured antibacterial activities regarding zone of inhibition in millimetres of all bicyclic ketones are presented in Table 3.…”

Section: Antibacterial Activity Assaymentioning

confidence: 99%

“…23 The mono-or di-or tri-or poly -OH (alcohols) and -OCH3 substituted organic compounds possess significant antioxidant activities. 24,25 Literature survey reveals that the synthesis and the study of pharmacological effects of 2-hydroxy-1-naphthyl based bicyclic methanones are almost absent in the past. Hence, the author has taken efforts to synthesize some 2-hydroxy-1-naphthyl based heptane[2.2.1]methanones by greener method for the evaluation of antimicrobial activity by Bauer-Kirby 26 disc diffusion method and the antioxidant activities by diphenyl picrylhydrazyl (DPPH) radical scavenging 27 activity ability.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Evaluation of Biological Activities of Some 2-Hydroxy-1-Naphthyl Bicyclo [2.2.1] Heptene-2-Yl-Methanones

Thirunarayanan¹

2017

ECB

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Totally twelve ((2-hydroxy-1-naphthyl))(3-(substituted phenyl)bicyclo [2.2.1]hept-5-ene-2-yl)methanones have been synthesized by fly-ash catalyzed water-mediated Diels-Alder [4+2] cycloaddition reaction. The synthesized bicyclic ketones were characterized by their physical constants, analytical and spectroscopic data. The antimicrobial and antioxidant activities of these bicyclic methanones were evaluated using Bauer-Kirby disc diffusion and diphenyl picrylhydrazyl(DPPH) radical scavenging technique. Most of the bicyclic methanones showed good antibacterial and antifungal activities against their microbial strains. The hydroxy-and methoxy-substituted methanones shows significant antioxidant activity. *Corresponding Authors

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“…A perspective tendency of modern pharmaceutical technology is the search for effective products based on natural biologically active substances, which due to multicomponent composition, have a wide range of pharmacological activity. They contain a number of natural antioxidants that are easily involved in the metabolic processes of the organism, are more affordable and characterized by low toxicity, mild influence, and the absence of side effects [1][2][3]. Such complex drugs are less toxic which is beneficial, and that distinguishes them from medicinal products of microbial and synthetic origin.…”

Section: Introductionmentioning

confidence: 99%

The Investigation of the Development of a Cream Composition With the Sapropel Extract

Strus

Polovko

Plaskonis

2018

Asian J Pharm Clin Res

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Objective: The objective of this research was the development of cream with sapropel extract on the emulsifying base and the investigation of the possibility for cream samples to be in future applied in medicine, cosmetics, and veterinary.Methods: To carry out the research a set of methods (centrifugal, thermal, and potentiometric) to analyze colloidal and thermal stability and determine pH values of the tested samples were used. Organoleptic characteristics of the tested samples and possible signs of physical instability were studied. Rheological properties of the samples were determined on the rotating viscometer. The method of microscopic analysis was carried out to analyze the stability of the emulsion system.Results: The research outcomes revealed that all the prototype samples have thermal and colloid stability, neutral pH value, and consistency that depends on the content of the oil phase and the emulsifying mixture. The samples, containing 15% of Vaseline oil and 4% and 6% of emulsifier no. 1, have low viscosity indexes, and the sample with 10% of emulsifier has a rather dense consistency. The samples containing 20% of oil phase possess unsatisfactory organoleptic properties. Since the sample with 4% of emulsifier possessed low rates of viscosity and phase separation occurred during its storage.Microscopic studies have shown that the sample containing 6% of emulsifier no. 1 exhibits monodisperse and a uniform roundness of drops that indicates the stability of the system.Conclusion: The outcome of the research is the development of the composition of the cream with sapropel extract with the emulsifying base containing 15% of corn oil, 6% of emulsifier no. 1, and 1% of Cetostearyl alcohol and purified water.

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Pharmacognostical Screening and Determination of Antioxidant Activity of Nelumbo Nucifera Gaertn Ethanol Seed Extract by Different in Vitro Models

Cited by 4 publications

References 23 publications

COMPARISON OF INHIBITORY ACTIVITY AGAINST THE Α-Glucosidase ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

COMPARISON OF INHIBITORY ACTIVITY AGAINST THE Α-Glucosidase ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

Synthesis and Evaluation of Biological Activities of Some 2-Hydroxy-1-Naphthyl Bicyclo [2.2.1] Heptene-2-Yl-Methanones

The Investigation of the Development of a Cream Composition With the Sapropel Extract

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