Pharmacokinetic applications of capillary electrophoresis: A review on recent progress

Sung, Wang Chou; Chen, Shuhui

doi:10.1002/elps.200500617

Cited by 7 publications

(3 citation statements)

References 90 publications

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“…Recent applications of CE in the analysis of pharmaceutical proteins have been reviewed by Little and his co-workers [17]. Chen and co-workers published serial review articles dealing with CE applications in pharmacokinetic investigations [18][19][20][21].…”

Section: Introductionmentioning

confidence: 98%

Impurity analysis of pharmaceuticals using capillary electromigration methods

Jouyban

Kenndler

2008

Electrophoresis

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This review deals with the determination of impurities in pharmaceuticals by electromigration methods in the capillary format. These separation methods are either based on the different effective mobility of the charged analytes (as in zone electrophoresis and isotachophoresis) or include hybrid methods such as micellar electrokinetic chromatography, microemulsion electrokinetic chromatography and electrochromatography. The pharmaceutically active compounds under consideration belong to chemotherapeutic agents, central nervous system drugs, histamine receptor drugs, cardiovascular drugs, anticancer drugs, anti-inflammatory drugs and some other drugs. The review discusses about 150 publications from the period between 1980 and 2007 with special emphasis on the recent trends and gives details about the experimental conditions applied for analyses and the obtained analytical performance parameters.

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Section: Introductionmentioning

confidence: 98%

Impurity analysis of pharmaceuticals using capillary electromigration methods

Jouyban

Kenndler

2008

Electrophoresis

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“…Due to its high efficiency and high speed of analysis, CE has been widely used in many fields, such as biochemistry [1][2][3][4], food analysis [5], pharmaceuticals [6], environmental monitoring [7], etc. In CE, samples can be injected either by a hydrodynamic (HD) or an electrokinetic (EK) method.…”

Section: Introductionmentioning

confidence: 99%

An electrical pumping approach to eliminate sample bias in capillary electrokinetic injection

Yang¹,

Bao²

2007

Electrophoresis

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A general pumping injection (PI), which involves the use of two capillaries with different diameters, was taken to evaluate systematically the effects on eliminating sample bias associated with the electrokinetic injection process in CE. One end of the separation capillary of the smaller diameter was inserted into another pumping capillary of larger diameter. When a high voltage was applied to the pumping capillary, the EOF generated inside will act as a pump to drive the solution stream in the separation capillary. The results have demonstrated that PI is suitable for both normal and reverse EOF situations. Second, the bias degree (BD) and SD of bias we presented were used to evaluate the degree of the bias under different conditions, and the factors of bias elimination have been investigated. Under optimal conditions, the bias was satisfactorily eliminated by PI. This EOF pumping system was successfully applied to the analysis of samples in CEC for a bias-free injection. Moreover, this two-capillary pumping system did not significantly affect the EOF, current, and the column efficiency of the separation process. Finally, a PI with grounded electrode was proposed and shown to be suitable for samples with low conductivity and ions with different mobility.

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“…CE has also frequently been used for the analysis of TCA drugs and for therapeutic drug monitoring due to the high peak efficiencies, small injection volumes, short separation times and ease of automation 23–25. The most common modes of CE for the analysis of TCA drugs are capillary zone electrophoresis (CZE) 26–32, non‐aqueous capillary electrophoresis (NACE) 33–36 and micellar electrokinetic capillary chromatography (MEKC) 37–39.…”

Section: Introductionmentioning

confidence: 99%

Optimizing the extraction, separation and quantification of tricyclic antidepressant drugs in human plasma with CE‐ESI‐TOF‐MS using cationic‐coated capillaries

et al. 2011

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In this study, the extraction and CE-ESI-TOF-MS analysis of tricyclic antidepressant (TCA) drugs imipramine, desipramine, clomipramine and norclomipramine in human plasma has been optimized. The CE capillaries were modified with ω-iodo-alkyl ammonium salt (M7C4I coating) to reduce analyte adsorption to the silica wall. The use of a strong cation exchange (SCX) solid-phase extraction (SPE) column specifically designed for the extraction of basic drug species from biofluids gave very clean extracts with high and reproducible recoveries. The extraction recoveries were ranging between 87 and 91% with % RSD values of 0.5-1.7% (n=3). The obtained strong cation exchange-SPE extracts of the TCA in human plasma only contained the analytes of interest. The optimized CE separation conditions were obtained by adding ACN and acetic acid to the sample while using an aqueous BGE. The CE-ESI-TOF-MS analysis was performed within 6 min for all TCA analytes under the optimized condition with peak efficiencies up to 1.4 x 10⁵ plates/m and an average % RSD of the migration times of the analytes of 0.3% (n=5). The presented method can readily be used for the extraction and quantification of basic drug species in human biological fluids and in pharmaceutical formulations.

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Pharmacokinetic applications of capillary electrophoresis: A review on recent progress

Cited by 7 publications

References 90 publications

Impurity analysis of pharmaceuticals using capillary electromigration methods

Impurity analysis of pharmaceuticals using capillary electromigration methods

An electrical pumping approach to eliminate sample bias in capillary electrokinetic injection

Optimizing the extraction, separation and quantification of tricyclic antidepressant drugs in human plasma with CE‐ESI‐TOF‐MS using cationic‐coated capillaries

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