Pharmacokinetics and depletion of florfenicol in striped catfish Pangasianodon hypophthalmus after oral administration

Phạm, Quang Vinh; Nguyen, Quang Thang; Devreese, Mathias; Croubels, Siska; Dang, Thi Hoang Oanh; Dalsgaard, Anders; Maita, Masashi; Phú, Trần Minh

doi:10.1007/s12562-023-01682-w

Cited by 1 publication

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Vietnamese fish production, the most commonly used antimicrobials are phenicols (florfenicol), tetracyclines (oxytetracycline) and sulfonamides ( Luu et al, 2021 ). In striped catfish farming the most commonly used antimicrobial is florfenicol ( Pham et al, 2023 ) and the aquaculture sector is said to be a genetic hotspot for gene transfer, including antimicrobial resistance genes ( Watts et al, 2017 ). It was therefore no surprise to see a high prevalence (47–70%) of antimicrobial resistance genes for these three antimicrobial groups commonly used in the production.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic analysis of Aeromonas dhakensis and Aeromonas hydrophila from diseased striped catfish fingerlings cultured in Vietnam

Erickson,

Khoi,

Hounmanou

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

IntroductionMotile Aeromonas septicemia (MAS) is a burden for striped catfish (Pangasius hypophthalmus) farmers in Vietnam. MAS can be caused by several species of Aeromonas but Aeromonas hydrophila is seen as the leading cause of MAS in aquaculture, but recent reports suggest that A. dhakensis is also causing MAS.MethodsHere we investigated the bacterial etiology of MAS and compared the genomic features of A. hydrophila and A. dhakensis. We collected 86 isolates from diseased striped catfish fingerlings over 5 years from eight provinces in Vietnam. Species identification was done using PCR, MALDI-TOF and whole genome sequence (WGS). The MICs of commonly used antimicrobials was established. Thirty presumed A. hydrophila isolates were sequenced for species confirmation and genomic comparison. A phylogenetic analysis was conducted using publicly available sequences and sequences from this study.ResultsA total of 25/30 isolates were A. dhakensis sequence type (ST) 656 and 5/30 isolates were A. hydrophila ST 251. Our isolates and all publicly available A. hydrophila isolates from Vietnam belonged to ST 251 and differed with <200 single nucleotide polymorphisms (SNP). Similarly, all A. dhakensis isolates from Vietnam belonged to ST 656 and differed with <100 SNPs. The tet(A) gene was found in 1/5 A. hydrophila and 19/25 A. dhakensis. All A. hydrophila had an MIC ≤2 mg/L while 19/25 A. dhakensis had MIC ≥8 mg/L for oxytetracycline. The floR gene was only found in A. dhakensis (14/25) which showed a MIC ≥8 mg/L for florfenicol. Key virulence genes, i.e., aerA/act, ahh1 and hlyA were present in all genomes, while ast was only present in A. dhakensis.DiscussionThis study confirms previous findings where A. dhakensis was the dominating pathogen causing MAS and that the importance of A. hydrophila has likely been overestimated. The differences in antimicrobial susceptibility between the two species could indicate a need for targeted antimicrobial treatment plans. The lipopolysaccharide regions and outer membrane proteins did not significantly differ in their immunogenic potentials, but it remains to be determined with in vivo experiments whether there is a difference in the efficacy of available vaccines against A. hydrophila and A. dhakensis.

show abstract

Section: Discussionmentioning

confidence: 99%