Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry

Wasfi, I.A.; Saeed, Hanan M.; Agha, Bushra; Kamel, Asmaa M.; Biriki, Nasreen A. Al; Neaimi, Khaled M. Al; Ali, Waleed A. Al; Sultan, Saeed Mahmoud

doi:10.1016/j.jchromb.2014.10.021

Cited by 6 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, dogs had a bioavailability of only 38% when administered at 5 mg/kg in fasted animals (FDA, 2004). Limited data are available on the use of oral and intravenous firocoxib in preruminating calves (Stock, Gehring, Barth, Wulf, & Coetzee, 2014;Stock et al, 2015), and a single publication details the use of intravenous firocoxib in adult camels (Wasfi et al, 2015).…”

mentioning

confidence: 99%

Pharmacokinetics and bioavailability of oral firocoxib in adult, mixed‐breed goats

Stuart

KuKanich

Caixeta

et al. 2019

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

Pharmacokinetic (PK) studies of oral firocoxib in large animal species have been limited to horses, preruminating calves, and adult camels. The aim of this study was to describe pharmacokinetics and bioavailability of firocoxib in adult goats. Ten healthy adult goats were administered 0.5 mg/kg firocoxib intravenously (i.v.) and per os (p.o.) in a randomized, crossover study. Plasma firocoxib concentrations were measured over a 96‐hr period for each treatment using HPLC and mass spectrometry, and PK analysis was performed. The p.o. formulation reached mean peak plasma concentration of 139 ng/ml (range: 87–196 ng/ml) in 0.77 hr (0.25–2.00 hr), and half‐life was 21.51 hr (10.21–48.32 hr). Mean bioavailability was 71% (51%–82%), indicative of adequate gastrointestinal absorption of firocoxib. There were no negative effects observed in any animal, and all blood work values remained within or very near reference range at the study's conclusion. Results indicate that oral firocoxib is well‐absorbed and rapidly reaches peak plasma concentrations, although the concentration also decreased quickly prior to the terminal phase. The prolonged half‐life may suggest tissue accumulation and higher plasma concentrations over time, depending on dosing schedule. Further studies to determine tissue residue depletion, pharmacodynamics, and therapeutic concentrations of firocoxib in goats are necessary.

show abstract

mentioning

confidence: 99%

Pharmacokinetics and bioavailability of oral firocoxib in adult, mixed‐breed goats

Stuart

KuKanich

Caixeta

et al. 2019

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

show abstract

“…Fitness of the camel for the study was determined as reported previously (Wasfi et al, 2015). Fitness of the camel for the study was determined as reported previously (Wasfi et al, 2015).…”

Section: Animalsmentioning

confidence: 99%

“…They were fed good quality hay and lucerne (alfalfa) once daily, with water allowed ad libitum. Fitness of the camel for the study was determined as reported previously (Wasfi et al, 2015).…”

Section: Animalsmentioning

confidence: 99%

“…Pharmacokinetic analysis for plasma furosemide for each animal was performed using least-squares nonlinear regression analysis program (WinNonLin Standard edition, version 1.5, USA) as described previously (Wasfi et al, 2015;Gibaldi & Perrier, 1982).…”

Section: Pharmacokinetic Analysismentioning

confidence: 99%

“…The respective experimental mass spectra with a 70,000 resolution of furosemide and furosemide-D5 are shown in (c), together with their respective 37 Cl isotope pattern of m/z 330.9985 and m/z 336.0294 for furosemide and furosemide-D5 respectively2.5 | Pharmacokinetic analysisPharmacokinetic analysis for plasma furosemide for each animal was performed using least-squares nonlinear regression analysis program (WinNonLin Standard edition, version 1.5, USA) as described previously(Wasfi et al, 2015;Gibaldi & Perrier, 1982).2.6 | Tentative identification of furosemide metabolitesA search of furosemide metabolites in extracted plasma samples was performed in a predictable manner, i.e. (a) Extracted ion chromatogram of the deprotonated monoisotopic accurate mass of furosemide (m/z 329.0004); (b) extracted ion chromatogram of the deprotonated monoisotopic accurate mass of furosemide D5 (m/z 334.0313).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Quantification of furosemide in camel plasma by high resolution mass spectrometry, application on pharmacokinetics

Wasfi¹,

Wajid²,

Agha³

et al. 2016

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

We developed and validated a high-resolution liquid chromatography mass spectrometry method for the quantification of furosemide in camel plasma which was used for a pharmacokinetic study in camels. Plasma samples were extracted by supported liquid extraction and furosemide and internal standard (furosemide-D5) were separated on a an Agilent Zorbax XDB C column (50 × 2.1 mm i.d., 3.5 μm). Data was acquired in full-scan mode over a mass range of 200-400 Da in negative electrospray mode at a resolution of 70,000. Linear calibration curves were obtained over the concentration ranges of 1.0-10,000 ng/mL. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolites of furosemide in six camels (Camelus dromedarus) and we were able to advice on a withdrawal time of furosemide treatment before racing.

show abstract

Non‐Steroidal Anti‐Inflammatory Drugs

Willette

2024

Pharmacology in Veterinary Anesthesia and Analgesia

View full text Add to dashboard Cite

Pharmacokinetics and metabolism study of firocoxib in camels after intravenous administration by using high-resolution bench-top orbitrap mass spectrometry

Cited by 6 publications

References 9 publications

Pharmacokinetics and bioavailability of oral firocoxib in adult, mixed‐breed goats

Pharmacokinetics and bioavailability of oral firocoxib in adult, mixed‐breed goats

Quantification of furosemide in camel plasma by high resolution mass spectrometry, application on pharmacokinetics

Non‐Steroidal Anti‐Inflammatory Drugs

Contact Info

Product

Resources

About