Pharmacokinetics and tissue depletion of doxycycline administered at high dosage to broiler chickens via the drinking water

Hsiao, Pai-Feng; Chang, Su-Wei; Hsu, Tien-Huan; Li, Kuang-Po; Chou, Chi‐Chung

doi:10.1556/004.2016.044

Cited by 13 publications

(9 citation statements)

References 12 publications

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“…The absorption profile does not differ appreciably from that observed in chickens given the same dose (Hantash et al., 2008 ; Hsiao et al., 2016 ; Soliman et al., 2015 ). In chickens, the peak plasma concentrations in three studies (4.7 µg/ml, 4.5 µg/ml, 5.4 µg/ml) occurred at 1.30 h, 2.07 h and 3.60 h, respectively (Hantash et al., 2008 ; Hsiao et al., 2016 ; Soliman et al., 2015 ), whereas in geese the C max (6.67 µg/ml) was reached in 2 h. Substantial differences were observed with the results of Anadón et al., 1994 , where the C max in broiler chickens (also administered 20 mg/kg) was much higher than that reported in geese (54 µg/ml) and achieved much faster (0.35 h).…”

Section: Discussionmentioning

confidence: 55%

“…Multip-e dose simulation of doxycycline PO administered to geese at 10 mg/kg (grey line, ) and 20 mg/kg (black line, -) daily for 5 days. The horizontal dashed line (---) represents the MIC value for M. gallisepticum (1.20 μg/ml) concentrations in three studies (4.7 µg/ml, 4.5 µg/ml, 5.4 µg/ml) occurred at 1.30 h, 2.07 h and 3.60 h, respectively (Hantash et al, 2008;Hsiao et al, 2016;Soliman et al, 2015), whereas in geese the C max (6.67 µg/ml) was reached in 2 h. Substantial differences were observed with the results of Anadón et al, 1994, where the C max in broiler chickens (also administered 20 mg/kg) was much higher than that reported in geese (54 µg/ml) and achieved much faster (0.35 h). Even ducks, another waterfowl species, had a different C max (17.57 µg/ml) when treated at the same dosage (Yang et al, 2015).…”

Section: F I G U R Ementioning

confidence: 99%

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Doxycycline pharmacokinetics in geese

Sartini

Łebkowska‐Wieruszewska

Lisowski

et al. 2021

Vet Pharm & Therapeutics

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Infection with Mycoplasma species is one of the main causes of significant economic loss in the domestic goose industry worldwide, causing reduced growth and egg production, and increased mortality and treatment costs (Stipkovits and Szathmary, 2012;Yadav et al., 2021). Waterfowl meat and eggs are highly valued in different countries worldwide, primarily due to their high nutritional quality.France, for instance, is the world's second-largest duck and goose meat consumer, being exceeded only by China Pingel, 2004). Moreover, wild birds, included ducks and geese, may play an important role as potential reservoirs and vectors of Mycoplasma species to the detriment of other domestic poultry. M. gallisepticum and M. synovitis are the most relevant strains among Mycoplasma species in poultry and are recognized as respiratory pathogens by the World Organization for Animal Health (OIE, 2018). Mycoplasma species are sensitive to a variety of antibacterials, including tetracyclines. Doxycycline is a semisynthetic tetracycline derivative with a broad spectrum of bacteriostatic activity. It acts by binding to the bacterial 30S ribosomal subunit, inhibiting

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Section: Discussionmentioning

confidence: 55%

Section: F I G U R Ementioning

confidence: 99%

Doxycycline pharmacokinetics in geese

Sartini

Łebkowska‐Wieruszewska

Lisowski

et al. 2021

Vet Pharm & Therapeutics

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“…Therefore, the use of clenbuterol as a treatment should proceed with logic and responsible application to the body [ 12 , 27 , 28 ]. If the calculation of withdrawal times was performed using the new method for calculating withdrawal times by substitute of Co in equation 1, with doses administered as accumulate drugs, the results would be satisfactory with no drug residues in all organs [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Calculate of withdrawal times of clenbuterol in goats to obtain safe times of slaughter

Lazuardi¹,

Hermanto²,

Restiadi³

2018

Vet World

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Background and Aim:Clenbuterol as a β2-agonist drug was investigated according to the concentration of the drug available in the bodies of goats and according to the level of sensitivity of the instruments used for detection. The objective of the current study was to determine withdrawal times after giving a therapeutic dose that resulted in safe slaughters.Materials and Methods:Five healthy male goats with a mean body weight of 20.64 kg were treated with a single dose of 5.10−3 mg/kg in the BW onto jugular vein. Whole blood samples of approximately 5 mL were taken in a time series at 5, 30, 60, 90, 150, 210, 270, 390, 510, 630, and 750 min. At 24 h posttreatment, all subjects were sacrificed, and 300 g samples of the liver were obtained. The plasma concentration and liver residue of the drug were observed by reverse-phase high-performance liquid chromatography.Results:The drug reached a maximum concentration of 19.233±0.331 µg/mL at 5 min, and the elimination half-life was at 173.25 min. The limit detection was obtained at 0.053 µg/mL. A one-way analysis of variance between all goats showed that elimination of the clenbuterol in their bodies was similar (p=1.00), with a withdrawal time of 1,479.326 min and no residues in the liver (p<0.05).Conclusion:Safe times for slaughter were determined to be at 2 days, 13 h, and 12 min as the 2nd safety factor (SF) time and 3 days, 1 h, and 58 min as the 3rd SF time with the liver organ free from residue.elimination half-life, new method for calculating withdrawal time, prescriptions for obtained β2-agonist, residues in liver.

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“…Для вивчення брали чисту плазму курей (попередньо перевірену методом УЕРХ-МС/МС на вміст доксицикліну), збагачували стандартним розчином доксицикліну на рівні 1 і 5 мкг/мл, і послідовно розводили в 5000 разів буфером для розведення зразків, що надається у наборі, для того, щоб отримані розчини, які за вмістом аналіту потрапляли в концентраційний інтервал калібрувальної кривої, і далі проводили аналіз імуноферментним методом згідно з інструкцією. Для навантаження обрали такі концентрації доксицикліну, порядок яких близький до вмісту його в плазмі крові курей-бройлерів при лікуванні їх препаратами на основі доксицикліну (Anadon et al, 1994;Laczay et al, 2001;Wijayanti et al, 2011;Hsiao et al, 2016;Pai-Feng at al., 2016;Yang et al, 2018). Для перевірки правильності та ефективності такого підходу, ці ж зразки було проаналізовано методом УЕРХ-МС/МС, використовуючи калібрувальний графік в інтервалі 0 -10,0 мкг/мл, побудований на матриці чистого зразка плазми крові курчат-бройлерів.…”

Section: рис 1 хімічна структура доксициклінуunclassified

Doxycycline Determination in Animal Tissues and Blood Plasma Samples Using Screening and Confirmatory Methods

Yanovych

Zasadna²,

Rydchuk

et al. 2021

SCIVP

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The article presents the results of validation of sample preparation method for muscle and parenchymal tissues of animals for further determination of doxycycline residual amounts by enzyme-linked immunosorbent assay using Oxytetracycline ELISA test kits from EuroProxima (Netherlands), initially developed for the analysis of other target matrices, viz. honey and shrimp. The results of modification of shrimp sample preparation method for the analysis of muscle and parenchymal tissue samples are showed. In accordance with the European Commission Decision 2002/657/EC, the proposed method was validated taking into account the maximum residue levels (MRLs) of doxycycline in tissues of productive animals: 100 μg/kg for muscle tissue and 300 μg/kg for liver. The suitability of the technique was confirmed on the basis of the established main validation parameters for screening methods (technical threshold and cut-off factor) using control (pure) and spiked with standard doxycycline solution at the level of ½MRL samples of muscle and parenchymal tissues of broiler chickens by means of “added – found” criterion. It was discovered that this approach to sample preparation makes it possible to determine doxycycline residues in poultry tissues at the level of 5-10 μg/kg with the absolute recovery about 54 %. The main advantages of the developed technique are simplicity of performance, rapidity and cost-effectiveness. The reliability of the results obtained by the screening method was verified using confirmatory UPLC-MS/MS method, viz. a comparative study of blank and spiked samples of muscle and parenchymal tissues of broiler chickens. The method of sample preparation of the studied specimens, the conditions of analysis carrying out, and the main validation parameters of the confirmatory method are also presented. Based on the obtained results, it was concluded that the developed screening method of enzyme-linked immunosorbent assay is suitable for the determination of doxycycline residual amounts in muscle and parenchymal tissues samples, and it is possible to use it for routine laboratory tests, as well as for the establishment of withdrawal periods of veterinary drugs based on doxycycline from the tissues of productive animals. Also using the same test kit, the sample preparation method for blood plasma samples for doxycycline content assay (at the levels of μg/ml) was suggested to study the bioequivalence of veterinary drugs containing doxycycline as an active pharmaceutical ingredient, which was as well confirmed by UPLC-MS/MS.

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Pharmacokinetics and tissue depletion of doxycycline administered at high dosage to broiler chickens via the drinking water

Cited by 13 publications

References 12 publications

Doxycycline pharmacokinetics in geese

Doxycycline pharmacokinetics in geese

Calculate of withdrawal times of clenbuterol in goats to obtain safe times of slaughter

Doxycycline Determination in Animal Tissues and Blood Plasma Samples Using Screening and Confirmatory Methods

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