Pharmacokinetics of eugenol in rainbow trout (Oncorhynchus mykiss)

Guénette, Sarah Annie; Uhland, F. Carl; Hélie, Pierre; Beaudry, Francis; Vachon, Pascal

doi:10.1016/j.aquaculture.2007.02.046

Cited by 68 publications

(50 citation statements)

References 25 publications

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“…In rainbow trout, recovery time was slightly longer 1.3 -8.1 × compared to induction time. This could be explained by the fact that eugenol exerts an inhibitory effect on respiration, reducing the ability of fish to remove the excess of eugenol from the system via the gills (Keene et al 1998;Guénette et al 2007). Our results indicate that clove concentration of 50 mg·l -1 induces the minimum induction and recovery times in rainbow trout; thus, this dose may be suitable for sedation and for standard on-farm procedures that require anaesthesia.…”

Section: Discussionmentioning

confidence: 99%

Size-relative Effectiveness of Clove Oil as an Anaesthetic for Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) and Goldfish (Carassius auratus Linnaeus, 1758)

et al. 2010

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The purpose of this work was to investigate the size-relative effectiveness of clove oil as an anaesthetic for rainbow trout and goldfish. In total, 128 rainbow trout (Oncorhynchus mykiss) (two groups of 20-23 and 30-33 cm mean fork length) and 160 goldfish (Carassius auratus) (four size groups of 1.5-2.5, 5-7, 11-15 and 20-25 cm) were anaesthetized at different clove oil concentrations of 50, 100, 150 mg·l -1 for trouts and 75, 100, 150 mg·l -1 for goldfish. Rainbow trout exhibited total loss of balance and no response to external stimuli with shorter induction time as dosage increased (120.5 s, 64.4 s and 44.3 s, respectively). Goldfish exhibited total loss of balance and no response to external stimuli after induction time that varied with dosage used and body size of fish. The small fish (1.5-7 cm) exhibited shorter induction time which ranged from 84.28 s at 75 mg·l -1 clove oil to 41.14 s at 150 mg·l -1 clove oil. The larger fish had a longer induction time inversely related to the dosage. Recovery time was longer than induction time in both species. Both species recovered within 6 min after anaesthesia at 150 mg·l -1 clove oil. Clove oil did not produce marked changes (P < 0.05) in the physiological indicators of goldfish compared to the control. However, marked changes (P < 0.05) were exhibited in the haematocrit of treated rainbow trout that also exhibited hyperkalaemia and hyperglycaemia (P > 0.05). For both fish species, clove oil was effective, producing minimum stress and zero mortalities, and can be recommended as an effective anaesthetic. Syzygium aromaticum, fish anaesthesia, animal welfare

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Section: Discussionmentioning

confidence: 99%

Size-relative Effectiveness of Clove Oil as an Anaesthetic for Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) and Goldfish (Carassius auratus Linnaeus, 1758)

et al. 2010

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“…Nesse aspecto, o eugenol possui vantagem sobre a benzocaína por ser rapidamente metabolizado e excretado, não havendo necessidade de depuração (CHO & HEATH, 2000). No entanto, estudos recentes revelam que a administração sucessiva de eugenol pode levar à acumulação do produto em trutas e que novos estudos devem ser conduzidos para determinar sua toxicidade (GUENETTE et al, 2007).…”

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Benzocaína e eugenol como anestésicos para juvenis do pampo Trachinotus marginatus

et al. 2008

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RESUMO O objetivo deste trabalho foi avaliar a eficácia da benzocaína e do eugenol como anestésicos para juvenis de Trachinotus marginatus. Foram testadas concentrações de 25, 50, 75, 100 e 150ppm de benzocaína e de 25, 37,5, 50, 62,5 Palavras-chave: anestesia, manejo, piscicultura marinha,Trachinotus marginatus. (25, 50, 75, 100, and 150ppm) and eugenol (25, 37.5, 50, 62.5, and 75ppm) were tested. The trials were conducted in glass aquariums filled with 30l seawater (salinity 30‰ and temperature 19°C) ABSTRACT The aim of this experiment was to evaluate the efficacy of benzocaine and eugenol as anesthetics for Trachinotus marginatus juveniles. Five concentrations of benzocaine

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“…It has been used as fish anaesthetics in the aquaculture industry and has shown to have low intoxication and mortality risks (Soto and Burhanuddin, 1995;Griffiths, 2000;Inoue et al, 2011). Moreover, the use of this anesthetic is advantageous because it is relatively inexpensive, efficacious, cost effectiveness, safe and does not present risks for users or to the environment (Soto and Burhanuddin, 1995;Munday and Wilson, 1997;Guénette et al, 2007;Hisano et al, 2008;Lucena et al, 2013;Öğretmen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes

et al. 2016

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Clove oil is used as a fish anesthetic because it is a natural and inexpensive product with low toxicity risks. The goal of the present study was to determine the appropriate concentration of clove oil for small-sized tropical fish to be used in mark-recapture studies or when individuals are to be sacrificed. We applied three different clove oil concentrations (D1=0.05 mL, D2=0.10 mL and D3=0.20 mL per 500 mL of water) on three small-sized fish species. We found a negative relationship between induction time and treatment for two species (Hyphessobrycon sp.1 and Hemigrammus sp.), while concentration was unrelated to recovery time. Fish body length was positively related to induction time in the D2 treatment for Hemigrammus sp., and negatively for Hyphessobrycon sp.1 in the D1 treatment, but was unrelated to recovery time for three species and treatments. Mortality rates varied across treatments, but higher rates were observed with higher clove oil concentrations. We conclude that 0.05 mL of clove oil per 500 mL of water is the most efficient dose for studies where fish will be released back to their natural habitats, while 0.20 mL of clove oil is recommended for studies that require fish euthanization for further laboratory analyses.Keywords: Eugenol, anesthesia, euthanasia, field experiment, fish manipulation.A eficiência do óleo de cravo como anestésico e em procedimentos de eutanaisa em peixes de pequeno porte Resumo O óleo de cravo é recomendado como anestésico para peixes por ser produto de origem natural, baixo custo e apresentar poucos riscos de intoxicação. O objetivo deste trabalho foi determinar concentrações adequadas de óleo de cravo para anestesiar ou eutanasiar peixes de pequeno porte em ambiente natural. Foram testadas três concentrações do anestésico (D1=0,05 mL, D2=0,10 mL e D3=0,20 mL) em três espécies de peixes de pequeno. Houve uma relação negativa entre o tempo para a sedação dos indivíduos e a concentração para duas espécies (Hyphessobrycon sp.1 e Hemigrammus sp.), porém não foi encontrada relação entre o tempo para recuperação e as concentrações. Os exemplares maiores de Hemigrammus sp. levaram mais tempo para serem sedados no tratamento D2, já o contrário foi observado para Hyphessobrycon sp.1 no tratamento D1, enquanto que não houve efeito do comprimento no tempo de recuperação das três espécies. A mortalidade dos indivíduos variou entre as três concentrações do anestésico e as maiores taxas de mortalidade ocorreram nas maiores concentrações. Desse modo, a concentração de 0,05 mL é eficiente para estudos que envolvem manuseio e a soltura dos peixes, enquanto que a concentração de 0,20 mL é recomendada em estudos onde os peixes precisam ser sacrificados.Palavras-chave: Eugenol, anestesia, eutanásia, experimento de campo, manipulação de peixes.

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Pharmacokinetics of eugenol in rainbow trout (Oncorhynchus mykiss)

Cited by 68 publications

References 25 publications

Size-relative Effectiveness of Clove Oil as an Anaesthetic for Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) and Goldfish (Carassius auratus Linnaeus, 1758)

Size-relative Effectiveness of Clove Oil as an Anaesthetic for Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) and Goldfish (Carassius auratus Linnaeus, 1758)

Benzocaína e eugenol como anestésicos para juvenis do pampo Trachinotus marginatus

The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes

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