Pharmacokinetics of tulathromycin on plasma and semen of beef bulls

Romano, Juan E.; Barbarossa, Andrea; Pagliuca, Giampiero; Villadóniga, Graciela B.; Gazzotti, Teresa; Mislei, Beatrice; Zironi, Elisa; Mari, Gaetano

doi:10.1016/j.theriogenology.2021.09.019

Cited by 7 publications

(3 citation statements)

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“…Figure 4 shows sample concentration versus time curves for the three matrices in one of the bulls enrolled in the study. Data obtained from the pharmacokinetic study are available elsewhere 20 …”

Section: Resultsmentioning

confidence: 99%

“…Two of the bulls received the dose at the base of the left ear and two in the middle of the left side of the neck. Samples of blood (in lithium heparin), semen, and urine were collected at 0, 12, 24, 48, 72, 96, 144, 192, and 240 h after tulathromycin administration 20 . All the samples were immediately refrigerated, centrifuged for 30 min at 600× g , and stored at −80°C until analysis in LC‐MS/MS.…”

Section: Methodsmentioning

confidence: 99%

“…Samples of blood (in lithium heparin), semen, and urine were collected at 0, 12, 24, 48, 72, 96, 144, 192, and 240 h after tulathromycin administration. 20 All the samples were immediately refrigerated, centrifuged for 30 min at 600Âg, and stored at À80 C until analysis in LC-MS/MS. During the analysis of each batch of the collected samples, a calibration curve and quality control samples were freshly prepared for each matrix as performed for validation, to confirm linearity, accuracy, and precision of the method.…”

Section: Application To a Pharmacokinetic Studymentioning

confidence: 99%

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A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull

Barbarossa

Bardhi

Gazzotti

et al. 2022

Drug Testing and Analysis

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Tulathromycin is a macrolide antibiotic generally used for the treatment of respiratory diseases in cattle and swine. This work proposes an improvement of a previously published LC-MS/MS method for tulathromycin determination in pig serum, here validated in three different bull matrices: plasma, seminal plasma, and urine. The approach is based on a quick protein precipitation with acetonitrile, filtration, and sample dilution before injection, allowing to rapidly process large batches of samples.Analytes separation was obtained using a BEH C18 (50 Â 2.1 mm, 1.7 μm) column, maintained at 40 C with a chromatographic run of 5 min. The method was fully validated over concentration ranges suitable for field levels of tulathromycin found in each matrix (0.01-1 μg/ml for plasma, 0.05-5 μg/ml for seminal plasma, and 0.1-10 μg/ml for urine), showing good linearity during each day of testing (R 2 always >0.99). Accuracy and precision were within ±15% at all QC concentrations in all the three matrices. Furthermore, the use of tulathromycine-d7 as internal standard mitigated the potential impacts of matrix effect. The validated technique was successfully applied to samples collected during a pharmacokinetic study in bulls, allowing to monitor tulathromycin concentrations over time in the three matrices. To our knowledge, this is the first validated approach for LC-MS/MS quantification of tulathromycin in seminal plasma and urine.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Application To a Pharmacokinetic Studymentioning

confidence: 99%

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