Pharmacological activities and molecular mechanisms of Pulsatilla saponins

Zhong, Jinmiao; Tan, Lihua; Chen, Meiwan; He, Chengwei

doi:10.1186/s13020-022-00613-8

Cited by 29 publications

(16 citation statements)

References 78 publications

(71 reference statements)

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“…Nonetheless, a 4.6-fold increase in necrotic cell death was observed. Our findings match earlier studies reporting the apoptotic activity of numerous naturally occurring triterpene saponins against cancer cells − …”

Section: Resultssupporting

confidence: 93%

A New Saponin (Zygo-albuside D) from Zygophyllum album Roots Triggers Apoptosis in Non-Small Cell Lung Carcinoma (A549 Cells) through CDK-2 Inhibition

et al. 2023

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Phytochemical study of the ethyl acetate root extract of Zygophyllum album has resulted in the isolation of a new saponin, Zygo-albuside D (1), along with two known compounds; (3-O-[β-D-quinovopyranosyl]-quinovic acid) (2), which is first reported in the root, and catechin (3), first reported in the genus. Their chemical structures were established by NMR and high-resolution mass spectrometry (HRMS). The new saponin (1) exhibited promising cytotoxicity with IC50 values of 3.5 and 5.52 μM on A549 and PC-3 cancer cell lines, respectively, compared to doxorubicin with IC50 values of 9.44 and 11.39 μM on A549 and PC-3 cancer cell lines, respectively. While it had an IC50 value of 46.8 μM against WISH cells. Investigating apoptosis-induction, compound 1 induced total apoptotic cell death in A549 lung cancer cells by 32-fold; 21.53% compared to 0.67% in the untreated control cells. Finally, it upregulated the pro-apoptotic genes and downregulated the antiapoptotic gene using gene expression levels. Compound 1 exhibited remarkable CDK-2 target inhibition by 96.2% with an IC50 value of 117.6 nM compared to Roscovitine. The molecular docking study further confirmed the binding affinity of compound 1 as CDK2 and Bcl2 inhibitors that led to apoptosis induction in A549 cancer cells. Hence, this study highlights the importance of compound 1 in the design of a new anticancer agent with specific mechanisms.

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Section: Resultssupporting

confidence: 93%

A New Saponin (Zygo-albuside D) from Zygophyllum album Roots Triggers Apoptosis in Non-Small Cell Lung Carcinoma (A549 Cells) through CDK-2 Inhibition

et al. 2023

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“…19 Additionally, Pulsatilla saponins have exhibited the ability to induce apoptosis in cancer cells, inhibit tumor angiogenesis, and provide organ protection through anti-inflammatory and antioxidant measures. 20 In this study, the results revealed a significantly higher total saponin content in 1-year-old PG roots compared to 6-month-old seedlings. These findings suggest that 1-year-old PG roots may possess greater medicinal value than 6-month-old seedlings.…”

Section: Total Saponin Contentsupporting

confidence: 47%

Effect of Developmental Stages on the Biosynthesis of Triterpene Saponins and Related Gene Expression in Seedlings of Platycodon grandiflorus

Liu,

Ren,

Wang

et al. 2023

Natural Product Communications

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Objective: To investigate the impact of developmental stages on triterpene saponin content and expression of key genes in their biosynthetic pathway in Platycodon grandiflorus seedlings. Method: The total saponins were extracted using ultrasonic extraction with methanol as the solvent. The quantification of Platycodin D in 6-month-old and 1-year-old Platycodon grandiflorus seedlings was performed using ultra-performance liquid chromatography tandem mass spectrometry, while the expression of key genes in the biosynthesis of saponins was determined by real-time quantitative polymerase chain reaction. Result: The content of Platycodin D and total saponins in 1-year-old Platycodon grandiflorus roots was significantly higher than that in of 6-month-old. The expression of key genes in saponin biosynthesis, such as PGMVK , PGMVD , PGDXR , PGMCT , PGMDS , PGHDS , PGHDR , PGFPS, and PGSS, was higher in 1-year-old roots compared to 6-month-old seedlings. Conclusion: The medicinal value of 1-year-old Platycodon grandiflorus root was higher than that of 6-month-old seedlings. The difference in medicinal value can be attributed to the variation in the expression of key genes involved in the saponin biosynthesis pathway.

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“…They protect vital organs as they are antioxidants and possess anti-inflammatory activities that mechanistically act by down-regulating the expression of TNF-α (Hu et al, 2009). They also have a role in regulating cell cycle, apoptosis, autophagy, angiogenesis, and they are reported to show anti-cancer activity (Zhong et al, 2022). The most abundant compounds in the natural products in general are terpenoids (Yang et al, 2020), however, the study findings reported low levels in terpenoids while a moderate level was reported by Njeru and Meshack (2016).…”

Section: Plant Extraction and Phytochemical Screeningmentioning

confidence: 99%

Phytochemical screening and gas chromatography-mass spectrometry analysis of Euphorbia ingens organic root extract

Innocent¹,

Florence²,

Mutinda³

et al. 2023

J. Med. Plants Res.

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Euphorbia ingens is a woody, succulent tree with ethnomedicinal applications; however, its chemical constituents are not well documented. This study determined the phytochemical profile of Euphorbia ingens dichloromethane-methanol root bark extract. Following sample collection and preparation, extraction was done using the cold percolation technique. This was followed by qualitative phytochemical screening and gas chromatography-mass spectrometry (GC-MS) analysis. This study reports relative high abundance of phenols, tannins and saponins with moderate and low levels of flavonoids and terpenoids respectively in E. ingens dichloromethane-methanol root bark extract. However, alkaloids, quinones and sterols were not identified in this study. Additionally, 14 phytocompounds were identified through GC-MS analysis, including undecane; copaene; 3,4-altrosan; 9, 12, 15-octadecatrienoic acid; hexadecanoic acid; 1,2-benzenedicarboxylic acid; lanosterol; androst-2en-17-one; lanosterol; 2-Bornanol; octadecanoic acid; squalene; 6-Pentylidene-4,5-secoandrostane-4,17.beta.-diol, and ethyl trans-4a, cis-4b, trans-8a, cis-10a-perhydro-trans-2,4a,8a-trimethyl-8oxophenanthrene-2-carbothiolate. These phytocompounds have been documented to have diverse pharmacological activities from antimicrobial, antioxidant, anticancer, hypercholesterolemic and antiinflammatory activities. Therefore, it was assumed in this study that these phytocompounds could be responsible for documented E. ingens pharmacological activities. Thus, the results of this study could support to some extent the ethnomedical uses of E. ingens. However, further biological investigations to validate the reported ethnobotanical uses of E. ingens are required.

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Pharmacological activities and molecular mechanisms of Pulsatilla saponins

Cited by 29 publications

References 78 publications

A New Saponin (Zygo-albuside D) from Zygophyllum album Roots Triggers Apoptosis in Non-Small Cell Lung Carcinoma (A549 Cells) through CDK-2 Inhibition

A New Saponin (Zygo-albuside D) from Zygophyllum album Roots Triggers Apoptosis in Non-Small Cell Lung Carcinoma (A549 Cells) through CDK-2 Inhibition

Effect of Developmental Stages on the Biosynthesis of Triterpene Saponins and Related Gene Expression in Seedlings of Platycodon grandiflorus

Phytochemical screening and gas chromatography-mass spectrometry analysis of Euphorbia ingens organic root extract

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