Pharmacological characterization of isoproterenol-treated medaka fish

Kawasaki, Takashi; Saito, Kotaro; Deguchi, Tomonori; Fujimori, Kazuhiro E.; Tadokoro, Mika; Yuba, Shunsuke; Ohgushi, Hajime; Kawarabayasi, Yutaka

doi:10.1016/j.phrs.2008.09.011

Cited by 20 publications

(12 citation statements)

References 23 publications

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“…There is general agreement that it is the β 1 AR subtype which is of 331 greatest functional importance in the heart [49], and similar to mammals the fathead minnow 332 β 1 AR receptors constitute 70% of the total βARs present in that tissue. The β 1 AR is also the predominant subtype in zebrafish heart [67], and functional studies have shown that the heart rate of zebrafish [56] and medaka [31] can be modulated by isoproterenol (agonist) and propranolol (antagonist). Although these agents are not β 1 AR specific [60], they would be anticipated to interact with the predominant βAR receptor present, and indeed adrb1 morpholino knock-downs resulted in reduced heart rate [67].…”

Section: Discussionmentioning

confidence: 99%

Characterisation and expression of β1-, β2- and β3-adrenergic receptors in the fathead minnow (Pimephales promelas)

Giltrow

Eccles

Hutchinson

et al. 2011

General and Comparative Endocrinology

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Complimentary DNAs for three beta-adrenergic receptors (βARs) were isolated and characterised in the fathead minnow. The encoded proteins of 402 (β(1)AR), 397 (β(2)AR) and 434 (β(3)AR) amino acids were homologous to other vertebrate βARs, and displayed the characteristic seven transmembrane helices of G Protein-coupled receptors. Motifs and amino acids shown to be important for ligand binding were conserved in the fathead minnow receptors. Quantitative RT-PCR revealed the expression of all receptors to be highest in the heart and lowest in the ovary. However, the β(1)AR was the predominant subtype in the heart (70%), and β(3)AR the predominant subtype in the ovary (53%). In the brain, β(1)AR expression was about 200-fold higher than that of β(2)- and β(3)AR, whereas in the liver, β(2)AR expression was about 20-fold and 100-fold higher than β(3)- and β(1)AR expression, respectively. Receptor gene expression was modulated by exposure to propranolol (0.001-1mg/L) for 21 days, but not in a consistent, concentration-related manner. These results show that the fathead minnow has a beta-adrenergic receptor repertoire similar to that of mammals, with the molecular signatures required for ligand binding. An exogenous ligand, the beta-blocker propranolol, is able to alter the expression profile of these receptors, although the functional relevance of such changes remains to be determined. Characterisation of the molecular targets for beta-blockers in fish will aid informed environmental risk assessments of these drugs, which are known to be present in the aquatic environment.

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Section: Discussionmentioning

confidence: 99%

Characterisation and expression of β1-, β2- and β3-adrenergic receptors in the fathead minnow (Pimephales promelas)

Giltrow

Eccles

Hutchinson

et al. 2011

General and Comparative Endocrinology

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“…Xiao et al, 1999;Bernstein, 2002). This dual coupling of 2AR might help explain why this receptor is not involved to the same extent as 1AR in G s -mediated cAMPexpression of ARs in the fish heart, and that fish hearts are responsive to classic AR ligands (Nickerson et al, 2001;Kawasaki et al, 2008;Mendonça and Gamperl, 2009;Steele et al, 2009). Some studies on fish ARs have also shown that they can have unexpected affinity for (presumed) subtype-specific agonists.…”

Section: Introductionmentioning

confidence: 97%

In vivo and in vitro assessment of cardiac β-adrenergic receptors in larval zebrafish (Danio rerio)

Steele

Yang

Debiais-Thibaud

et al. 2011

Journal of Experimental Biology

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SUMMARY β-Adrenergic receptors (βARs) are crucial for maintaining the rate and force of cardiac muscle contraction in vertebrates. Zebrafish (Danio rerio) have one β1AR gene and two β2AR genes (β2aAR and β2bAR). We examined the roles of these receptors in larval zebrafish in vivo by assessing the impact of translational gene knockdown on cardiac function. Zebrafish larvae lacking β1AR expression by morpholino knockdown displayed lower heart rates than control fish, whereas larvae deficient in both β2aAR and β2bAR expression exhibited significantly higher heart rates than controls. These results suggested a potential inhibitory role for one or both β2AR genes. By using cultured HEK293 cells transfected with zebrafish βARs, we demonstrated that stimulation with adrenaline or procaterol (a β2AR agonist) resulted in an increase in intracellular cAMP levels in cells expressing any of the three zebrafish βARs. In comparison with its human βAR counterpart, zebrafish β2aAR expressed in HEK293 cells appeared to exhibit a unique binding affinity profile for adrenergic ligands. Specifically, zebrafish β2aAR had a high binding affinity for phenylephrine, a classical α-adrenergic receptor agonist. The zebrafish receptors also had distinct ligand binding affinities for adrenergic agonists when compared with human βARs in culture, with zebrafish β2aAR being distinct from human β2AR and zebrafish β2bAR. Overall, this study provides insight into the function and evolution of both fish and mammalian β-adrenergic receptors.

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“…The OMR has also been used efficiently in medaka to investigate spectral sensitivity ( Hasegawa, 1998 ), athletic capability ( Kawasaki et al, 2008 ), and cohesive movement ( Imada et al, 2010 ). These small fish school in nature, indicating their preference to associate with moving objects, such as vertical stripes; i.e.…”

Section: Introductionmentioning

confidence: 99%

A semi-automatic and quantitative method to evaluate behavioral photosensitivity in animals based on the optomotor response (OMR)

et al. 2018

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The optomotor response (OMR) is a locomotor behavior of animals that is induced by moving repetitive visual stimuli. This characteristic helps animals particularly when stabilizing and maintaining position in schools and herds. Here, we developed a simple but sensitive method for quantifying the OMR using medaka (Oryzias latipes) as a model. This method, which simply requires video-recorded behavior, free tracking software, and a generic spreadsheet program, enables the evaluation of spectral sensitivity by researchers with little knowledge about the behavioral characteristics of the test animal or of the OMR. Based on a manual method, we reported previously that wild-type and red-colorblind medaka exhibited an OMR up to λ=830 and 740 nm, respectively. However, the present method, which quantifies the OMR according to three parameters (starting time, duration, and total distance of swimming) that are calculated based on a series of x–y coordinates of the moving fish, supported that conclusion and further indicated that both strains perceive light at even longer wavelengths. This low-cost, quantitative, and semi-automatic method would widen the opportunities to unveil behavioral photosensitivity in animals of interest..

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Pharmacological characterization of isoproterenol-treated medaka fish

Cited by 20 publications

References 23 publications

Characterisation and expression of β1-, β2- and β3-adrenergic receptors in the fathead minnow (Pimephales promelas)

Characterisation and expression of β1-, β2- and β3-adrenergic receptors in the fathead minnow (Pimephales promelas)

In vivo and in vitro assessment of cardiac β-adrenergic receptors in larval zebrafish (Danio rerio)

A semi-automatic and quantitative method to evaluate behavioral photosensitivity in animals based on the optomotor response (OMR)

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