Pharmacological effect of amphotericin B and its analogue A21 in a murine candidiasis model

Abstract

Our aim was to compare analogue A21 vs amphotericin B (AmB) in a mouse model of candidiasis. For the study, we used male Balb/c mice, which were randomly divided into six groups: control, candidiasis, AmB Indofine 4mg/kg, AmB Abelcet®, analogue A21 4 mg/kg and analogue A21 12 mg/kg. Candidiasis model was carried out by inoculated 5×107UFC/mL of Candida albicans 10231 yest P.O., 23 days after the treatment was given for the group along two weeks. Mice were sacrificed and liver, small intestine, lung and kidney were collected and then sectioned and fixed in phosphate‐buffered 4% formaldehyde for histological analysis. Samples were stained with hematoxylin‐eosin. Tissues with candidiasis showed alterations in their structures, in kidney, the number of glomeruli and tubules were decreased, while in the liver showed necrosis and apoptosis areas, also in intestine and lung showed alteration on their architecture. The analogue A21 at two concentrations impoved the structure of lungs and intestine, in kidney improved the structure and increase the number of glomeruli as AmB, in liver improved the structure at 12mg/kg. In conclusion, the analogue A21 improves the structure of tissues damaged by candidiasis like amphotericinB.