Pharmacological Evaluation of Mentha piperita Against Urolithiasis: An In Vitro and In Vivo Study

Obesity, a chronic metabolic condition, is an increase in fat mass and blood lipid levels mainly causing atherosclerosis and hypertension, which further lead to cardiovascular complications. The objective of the study was to investigate the crude extract of Caralluma edulis (CE.Cr) for its potential against high-fat diet (HFD)-induced obesity and its related complications. Hyperlipidemia was induced in Wistar albino rats with HFD (1% cholesterol + 0.5% cholic acid) for 28 days. Treatment groups were administered with different doses of CE.Cr (100, 300 and 500 mg/Kg, p.o.) and the standard group received atorvastatin. At the end of study, sera were analyzed for biochemical markers and the aorta was dissected for microscopic examination. Antioxidant potential was evaluated and high-performance liquid chromatography (HPLC) analysis was performed. The hypotensive potential of CE.Cr was evaluated through an invasive technique. HPLC analysis of CE.Cr showed the presence of chlorogenic acid, caffeic acid, apigenin and naringenin. Histological examination of the aorta section showed anti-atherosclerotic effects which were also evident from decrease in serum total cholesterol, triglycerides and low-density lipoproteins levels. CE.Cr decreased mean arterial blood pressure and evoked significant hypotensive effects. The crude extract of C. edulis showed anti-obesity, antihypertensive, anti-atherosclerotic and antioxidant potential.

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“…Sodium and potassium urinary concentrations were measured by using a clinical flame photometer (Model 410C, Sherwood, UK). 31 …”

Section: Methodsmentioning

confidence: 99%

Evaluation of Caralluma edulis for its Potential Against Obesity, Atherosclerosis and Hypertension

et al. 2023

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“…The effects of ACV on CaOx crystallization were investigated using a nucleation test [ 35 ]. The solutions of calcium chloride (0.005 M) and sodium oxalate (0.0075 M) were created in a buffer of Tris-HCL (0.5 M) and NaCl (0.15 M) at pH 6.5.…”

Section: Methodsmentioning

confidence: 99%

GC–MS Based Metabolite profiling, Antioxidant and Antiurolithiatic Properties of Apple Cider Vinegar

Singh

Chitra

et al. 2023

Future Sci. OA

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Aim: To perform in vitro analysis of antioxidant and antiurolithiasis to carry out GC–MS-based metabolite profile. Materials & methods: The effect of apple cider vinegar (ACV) in vitro, antioxidant and GC–MS analysis was evaluated. The antioxidant studies were performed. In vitro techniques included nucleation, aggregation and growth assay. Results & conclusion: The presence of polyphenols, flavonoids, alkaloids and carbohydrates was shown. Concentrations from 5–30 μg/ml could dissolve calcium oxalate (p < 0.05) in vitro. The IC50 value of ACV in DPPH was found to be around 7 μg/ml and the IC50 value of the ACV in ABTS assay was around 9 μg/ml. Different phytocompounds were obtained from GC–MS analysis. ACV can be consumed to prevent kidney stones which seems helpful to the current therapy.

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Section: Methodsmentioning

confidence: 99%

“…The mortality was noted after 14 days and behavioral changes were closely observed such as hyperactivity, tremors, ataxia, salivation, convulsions, diarrhea, lethargy, sleep, coma, and somatomotor activity. 18 In-vitro Clot Lysis/Thrombolysis Assay Stock solution of streptokinase (SK 30,000 IU/100µl) was prepared. Human blood (5 ml) was drawn from subjects without history of anticoagulants and oral contraceptives.…”

Section: Acute Toxicity Assaymentioning

confidence: 99%

Pharmacological Investigation of Asphodelus tenuifolius Cav. for its Potential Against Thrombosis in Experimental Models

2022

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Background Thrombosis is a major disorder which is an outcome of an imbalance in the hemostatic system that develop undesirable blood clot and hinder blood circulation. Purpose The current study was designed to verify the potential of aqueous methanolic crude extract of Asphodelus tenuifolius Cav . (At.Cr), used traditionally as remedy in circulatory problems. Research Design Antioxidant activity, FTIR, and HPLC analysis were performed. In-vitro clot lysis assay was performed on human blood samples, and in-vivo acute pulmonary thromboembolism model was developed by administering the mixture of collagen and epinephrine in tail vein of mice. Carrageenan-induced thrombosis and FeCl 3 -induced carotid arterial thrombosis models were developed in rats. Results At.Cr demonstrated significant increase in lysis of human blood clot. Bleeding and clotting times were increased dose-dependently. Lungs histology showed clear alveolar spaces with decreased red blood cells congestion. Reduction in infarcted tail length, augmentation in prothrombin time, and activated partial thromboplastin time with decrease in platelet count were observed. At.Cr also prolonged the arterial occlusion time and reduced the weight of thrombus and TXB 2 levels dose-dependently. Conclusions The results demonstrated the antithrombotic and thrombolytic potential of At.Cr due to activation of coagulation factors through extrinsic and intrinsic pathways.

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Pharmacological Evaluation of Mentha piperita Against Urolithiasis: An In Vitro and In Vivo Study

Cited by 14 publications

References 32 publications

Evaluation of Caralluma edulis for its Potential Against Obesity, Atherosclerosis and Hypertension

Evaluation of Caralluma edulis for its Potential Against Obesity, Atherosclerosis and Hypertension

GC–MS Based Metabolite profiling, Antioxidant and Antiurolithiatic Properties of Apple Cider Vinegar

Pharmacological Investigation of Asphodelus tenuifolius Cav. for its Potential Against Thrombosis in Experimental Models

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