Pharmacological properties of amino‐oxyacetic acid in the chicken

Osuide, G.

doi:10.1111/j.1476-5381.1972.tb07235.x

Cited by 15 publications

(7 citation statements)

References 22 publications

(24 reference statements)

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“…Similarly, high oxygen pressure produced a less notable decrease of GABA levels in the brains of 2-day-old chicks than in 22-day-old chicks (Wood, 1970). The convulsant effect of aminooxyacetic acid (Sections 2 and 5) is more notable in young chicks than in adults (Osuide, 1972).…”

Section: Effects Of Compounds On Gaba Metabolism During Ontogenymentioning

confidence: 95%

Biochemical Pharmacology of GABA in CNS

Tapia

1975

Amino Acid Neurotransmitters

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Section: Effects Of Compounds On Gaba Metabolism During Ontogenymentioning

confidence: 95%

Biochemical Pharmacology of GABA in CNS

Tapia

1975

Amino Acid Neurotransmitters

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“…Much of this work has been aimed at inhibiting brain 4-aminobutyrate transaminase (EC 2.6.1.19) to increase concentrations of the neurotransmitter 4-aminobutyrate (e.g. Baxter & Roberts, 1961;Osuide, 1972). The general reactivity of amino-oxyacetate towards pyridoxal phosphate-dependent enzymes is well recognized, since it is known that the compound reacts with 4-aminobutyrate transaminase (Wallach, 1961;Baxter & Roberts, 1961;Wu, 1976), alanine transaminase (EC 2.6.1.2; Hopper & Segal, 1962), glutamate decarboxylase (EC 4.1.1.15; Roberts & Simonsen, 1963), alanine racemase (EC 5.1.1.1; Free et al, 1967), histidine decarboxylase (EC 4.1.1.22; Leinweber, 1968), D-amino acid transaminase (EC 2.6.1.6;Yonaha et al, 1975) and cystathionase (EC 4.4.1.1; Beeler & Churchich, 1976).…”

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confidence: 99%

The reaction of amino-oxyacetate with pyridoxal phosphate-dependent enzymes

John¹,

Charteris²

1978

Biochemical Journal

139

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The carbonyl reagent amino-oxyacetate is frequently used in metabolic studies to inhibit individual pyridoxal phosphate enzymes. The reaction of this compound with three such enzymes, aspartate transaminase, 4-aminobutyrate transaminase and dopa (3,4-dihydroxyphenylalanine) decarboxylase, was studied to determine the extent to which the inhibition is reversible and the rates at which it takes place. Reactions were followed by observing changes in the absorption spectra of the bound coenzyme and by measuring loss of enzyme activity. The reactions with aspartate transaminase and aminobutyrate transaminase were not rapidly reversible and had second-order rate constants (21 degrees C) of 400 M-1.s.1 and 1300 M-1.s-1 respectively and all all concentrations studied showed the kinetics of a simple bimolecular reaction. The reaction with 4-aminobutyrate transaminase could not be reversed and that with aspartate transaminase could only be reversed significantly by addition of cysteinesulphinate to convert the enzyme into its pyridoxamine form. The first-order rate constant (21 degrees C) for the reverse reaction was 4 X 10(-5)s-1. Dopa decarboxylase inhibition by amino-oxyacetate was more rapid and more readily reversible, but measurements of rate and equilibrium constants were not obtained for this enzyme.

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“…It seems that in this experiment Apo and AOAA act synergistically: the action of Apo given alone was weak and short-lasting; AOAA by itself was even less potent. The literature points out that the effective anticonvulsive dose of AOAA is about ten times higher than that used in this experiment (Osuide, 1972;Wood and Peeske 1973).…”

Section: Behavioral Effects (Table 4)mentioning

confidence: 80%

Effect of Combined Dopaminergic and GABA‐ergic Stimulation on Ouabain‐Induced Epileptiform Activity

Stach

Kacz

1977

Epilepsia

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In this study we tested a hypothesis that dopamine and GABA receptor-stimulating agents given together might mutually potentiate their anticonvulsant activities. To test this hypothesis, experiments were performed on free-moving rabbits with epileptiform activity evoked by cortical application of ouabain. After combined treatment with apomorphine and GHBA 3 out of 6 animals displayed no EEG epileptiform activity, and in the remaining 3 rabbits this activity was greatly reduced. A combined pretreatment with apomorphine and GHBA abolished epileptiform EEG activity during the first hour and shortened it during the second hour, but not significantly. Apomorphine given together with amino-oxyacetic acid before ouabain completely abolished the epileptiform activity in 4 out of 6 animals. In the remaining 2 rabbits the latency of ouabain affect was prolonged, the epileptiform activity during the first hour was abolished, and it was markedly decreased during the next 2 hr (significantly from controls or groups receiving apomorphine or amino-oxyacetic acid). Phenytoin was less potent than apomorphine with GHBA or apomorphine given together with AOAA. The animals' behavior was observed.

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Pharmacological properties of amino‐oxyacetic acid in the chicken

Cited by 15 publications

References 22 publications

Biochemical Pharmacology of GABA in CNS

Biochemical Pharmacology of GABA in CNS

The reaction of amino-oxyacetate with pyridoxal phosphate-dependent enzymes

Effect of Combined Dopaminergic and GABA‐ergic Stimulation on Ouabain‐Induced Epileptiform Activity

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