Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from <i>Calycotome villosa</i> in rats

Cherkaoui-Tangi, Khadija; Lachkar, Mohammed; Wibo, Maurice; Morel, Nicole; Gilani, Anwarul Hassan; Lyoussi, Badiaâ

doi:10.1002/ptr.2322

Cited by 21 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intermedia [ 3 ] but this is the first time it has been isolated from this species. Previous studies showed hypotensive and diuretic properties of this compound and they revealed an ability to increase the α-transcriptional activity in MCF-7 cells [ 42 , 43 ]. In our work, a weak antioxidant activity toward the radical DPPH .…”

Section: Discussionmentioning

confidence: 99%

Isolation of Bioactive Compounds from Calicotome villosa Stems

et al. 2018

View full text Add to dashboard Cite

A phenylethanoid, two steroids, a flavone glucoside and a chalcone have been isolated for the first time from the stems of Calicotome villosa together with a previously isolated flavone glucoside. Their structures were determined by spectroscopic analyses (NMR, HRMS) as basalethanoïd B (1), β-sitosterol and stigmasterol (2), chrysine-7-O-β-d-glucopyranoside (3), chrysine 7-((6′′-O-acetyl)-O-β-d-glucopyranoside) (4) and calythropsin (5). The crude extracts and the isolated compounds (except 4), were evaluated for their antioxidant, antimicrobial (against two Gram-positive bacterial strains: Staphylococcus aureus, Bacillus cereus, four Gram-negative bacterial strains: Staphylococcus epidermidis, Klebsiella pneumonia, Acinetobacter baumanii, and three yeasts: Candida albicans, Candida tropicalis, and Candida glabrata), hemolytic, antidiabetic, anti-inflammatory and cytotoxic activity. The crude extracts showed good ability to scavenge the free radical DPPH. Methanol stem extract followed by the dichloromethane stem extract showed moderate antimicrobial potency; furthermore, at 1 mg/mL the methanol extract showed an inhibition of C. albicans growth comparable to nystatin. Dichloromethane, methanol, and aqueous extracts inhibited 98%, 90%, and 80% of HeLa cell proliferation at 2 mg/mL respectively. Weak hypoglycemic and hemolytic effects were exhibited by the crude extracts. Among all the tested compounds, compound 3 showed remarkable hypoglycemic potential (93% at 0.1 mg/mL) followed by compound 5 (90% at 0.3 mg/mL). Compound 5 was the most effective in the DPPH. scavenging assay (100% at 0.1 mg/mL) and cytotoxic assay on HeLa cells (99% and 90% after 24 and 48 h of treatment at 0.1 mg/mL, respectively). No anti-inflammatory effects were displayed by any of the crude extracts or the isolated compounds at any of the tested concentrations.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation of Bioactive Compounds from Calicotome villosa Stems

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Additionally, alkaloids have also been found to exhibit a vasoconstrictive 42 effect mediated through the release of Ca +243 and through endotheliumdependent pathway. 44 Glucosides and sesquiterpenes 45 are also known to possess an inhibitory effect on cardiovascular system. The current findings on the cardiovascular activities of Acorus calamus can be possibly correlated to its chemical constituents, particularly alkaloids in addition to glucosides and sesquiterpenes, which might be responsible for the antihypertensive and vasomodulatory effects because the other chemical constituents such as acorine and asarone are not known to possess effects related to the cardiovascular system.…”

Section: Discussionmentioning

confidence: 99%

Blood Pressure-lowering and Vascular Modulator Effects of Acorus calamus Extract Are Mediated Through Multiple Pathways

Shah

Gilani²

2009

Journal of Cardiovascular Pharmacology

View full text Add to dashboard Cite

This investigation was aimed to provide a pharmacologic basis to the medicinal use of Acorus calamus in cardiovascular disorders. In normotensive anesthetized rats, crude extract of Acorus calamus and its ethylacetate and nHexane fractions caused a fall in mean arterial pressure. In rabbit aorta rings, crude extract was more potent against high K (80 mM), ethylacetate against phenylephrine (1 microM), whereas nHexane fraction was equipotent against both precontractions. Crude extract exhibited a vasoconstrictor effect on baseline. Pretreatment of aortic rings with crude extract and its fractions shifted Ca concentration-response curves to the right, similar to verapamil. Crude extract and ethylacetate fraction suppressed phenylephrine peak formation in Ca-free medium. In rat aorta preparations, crude extract exhibited endothelium-independent relaxation with a vasodilatory effect against high K. nHexane fraction caused an endothelium-dependent Nomega-nitro-l-arginine methyl ester-sensitive vasorelaxant along with ryanodine-sensitive vasoconstrictor effect on baseline tension and partially inhibited high K, although ethylacetate fraction caused an endothelium-independent relaxant and endothelium-dependent vasoconstrictor effect. These data indicate that crude extract possesses a combination of effects, relaxant effects mediated possibly through Ca antagonism in addition to a nitric oxide pathway, although the associated vasoconstrictor effects may be meant by nature to offset excessive vasodilatation, thus providing a pharmacologic rationale to its cardiovascular medi-cinal uses.

show abstract

“…1), is known for its various biological activities (6,7). Previous studies have suggested that chrysin possesses antioxidant (8), antihypertensive (9), antidiabetogenic (10) and anxiolytic properties (11). In addition, chrysin exerts a strong anticancer effect in multiple types of human cancer, since it induces cell cycle arrest and cell apoptosis (12); however, whether chrysin may be used as a novel antitumor drug against HCC has not been extensively investigated.…”

Section: Introductionmentioning

confidence: 99%

Chrysin induces cell apoptosis via activation of the p53/Bcl-2/caspase-9 pathway in hepatocellular carcinoma cells

Zhang

Liu

et al. 2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Chrysin is a major active ingredient of flavonoids, known to exhibit protective effects against various types of cancer. However, the anticancer role of chrysin against hepatocellular carcinoma (HCC) and the underlying molecular mechanisms remain unclear. In order to evaluate the effects of chrysin on cell viability and apoptosis in human HCC, HepG2 and QGY7701 cells were used in the present study. Cell viability was monitored using an MTT assay. In addition, an Annexin V-fluorescein isothiocyanate/propidium iodide kit was used for the labeling of the apoptotic cells, which were then measured using flow cytometry. Western blotting was used to examine the protein expression of p53, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), Bcl-2-associated death promoter (Bad), Bcl-2 homologous antagonist/killer (Bak), caspases-3 and -9, and cleaved-caspases-3 and -9. The results of the present study revealed that chrysin suppressed the cell viability of HepG2 and QGY7701 cells in a concentration-dependent manner. In addition, chrysin induced significant apoptosis in HepG2 and QGY7701 cells. Furthermore, it was demonstrated that chrysin treatment increased the expression of proapoptotic proteins, including p53, Bax, Bad and Bak, while it decreased the protein level of antiapoptotic protein Bcl-2. It was also demonstrated that chrysin induced apoptosis in the HCC cells by regulating the p53/Bcl-2/caspase-9 signaling pathway. In conclusion, the results of the present study suggested that chrysin may be a potential candidate agent for the induction of cell apoptosis in human HCC.

show abstract

Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from Calycotome villosa in rats

Cited by 21 publications

References 18 publications

Isolation of Bioactive Compounds from Calicotome villosa Stems

Isolation of Bioactive Compounds from Calicotome villosa Stems

Blood Pressure-lowering and Vascular Modulator Effects of Acorus calamus Extract Are Mediated Through Multiple Pathways

Chrysin induces cell apoptosis via activation of the p53/Bcl-2/caspase-9 pathway in hepatocellular carcinoma cells

Contact Info

Product

Resources

About