Pharmakognosie — Phytopharmazie

Hänsel, Rudolf; Sticher, Otto; Steinegger, E.

doi:10.1007/978-3-662-09269-9

Cited by 150 publications

(163 citation statements)

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“…In contrast to other saponins, glycyrrhizic acid, which is used in Japan for the intravenous treatment of chronic hepatitis [25], exerts a very low haemolytic activity (haemolysis index <2000) [14]. The LD 50 values of monoammonium glycyrrhizinate were 325 and 478 mg/kg body weight in mice and rats, respectively [26].…”

Section: Discussionmentioning

confidence: 99%

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Saponins increase susceptibility of vancomycin-resistant enterococci to antibiotic compounds

Schmidt¹,

Heimesaat²,

Fischer³

et al. 2014

European Journal of Microbiology and Immunology

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The resistance of commensal bacteria to first and second line antibiotics has reached an alarming level in many parts of the world and endangers the effective treatment of infectious diseases. In this study, the influence of the plant-derived natural saponins glycyrrhizic acid, β-aescin, α-hederin, hederacoside C, and primulic acid 1 on the susceptibility of vancomycin-resistant enterococci (VRE) against antibiotics of clinical relevance was investigated in 20 clinical isolates. Furthermore, the antibacterial properties of saponins under study against VRE were determined in vitro. Results reveal that the susceptibility of VRE against gentamicin, teicoplanin, and daptomycin was enhanced in the presence of the saponin glycyrrhizic acid. Most importantly, glycyrrhizic acid (1 mg/ml) diminished the minimal inhibitory concentration (MIC) of gentamicin in gentamicin low-level intrinsic resistant VRE from 2 ->8 mg/l to ≤ 0.125-1 mg/l. The adding of β-aescin, α-hederin, hederacoside C, and primulic acid 1 to the antibiotics under study showed, compared to glycyrrhizic acid, less influence on the antibiotic potency. Only glycyrrhizic acid (1 mg/ml) and α-hederin (0.2 mg/ml) showed weak antibacterial properties against the clinical isolates. Our study points towards a therapeutic potential of saponins in the coapplication with antibiotics for bacterial infections.

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Section: Discussionmentioning

confidence: 99%

“…The amphoteric qualities resulting from it are responsible for many effects of the saponins. In the plant kingdom, they are widespread and can be found in several plants in concentrations up to 30% [14]. On account of their antibacterial and antifungal effects, saponins presumably serve the plants for defence against infections [15,16].…”

Section: Introductionmentioning

confidence: 99%

Saponins increase susceptibility of vancomycin-resistant enterococci to antibiotic compounds

Schmidt¹,

Heimesaat²,

Fischer³

et al. 2014

European Journal of Microbiology and Immunology

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“…It is native to northernmost Africa Europe and western Asia (Hänsel and Sticher 2007). Previous studies have demonstrated that crude extract of R. frangula has strong antioxidant, antimicrobial, and free radical scavenging activities (Manojlovic et al 2005;Ş tef et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

et al. 2017

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Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was selected as a protease inhibitor source. The maximum recovery of the protease inhibitor against trypsin was recorded in the crude extract made in 0.1 M phosphate buffer (pH 7.0) and isolated from the mature leaves. Then, the protease inhibitor designated as RfIP1 was purified to homogeneity by Sephadex G50 with an apparent molecular mass of 22.5 kDa and its N-terminal sequence exhibited a high degree of homology with known serine protease inhibitor sequences. The RfIP1 displayed maximal activity at pH 7 and 37°C. It maintained almost 80% of its maximal activity through a large pH range. The thermo-stability of RfIP1 was markedly enhanced by BSA, CaCl 2, and sorbitol, whereas the addition of Mg 2? , Zn 2? , NaTDC, SDS, DTT, and b-ME significantly promoted inhibitory activity. The protease inhibitor displayed high inhibitory activity toward some known proteases (cathepsin B, chymotrypsin, collagenase, thrombin, and trypsin) that have more importance in pharmaceutical industry and it acted as potent inhibitor of some commercially proteases from Aspergillus oryzae, Bacillus sp, and Bacillus licheniformis. The protease inhibitor also possessed an appreciable antibacterial effect against both Gram-positive and Gram-negative bacteria.

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“…Teores de até 1% de cinzas insolúveis em ácido são aceitáveis, considerando vegetais que não sejam ricos em ácido silícico ou silicatos. O caule de C. ferrea contém uma quantidade considerável de cinzas totais (5,31%), o que pode ser explicado pela presença de sais inorgânicos no material vegetal (HÄNSEL et al, 1999).…”

Section: Resultsunclassified

Caracterização físico-química e biológica do caule de Caesalpinia ferrea Mart.

Frasson¹,

Bittencourt²,

Heinzmann³

2003

Rev. bras. farmacogn.

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RESUMO: Visando estabelecer parâmetros para o controle da qualidade do caule de Caesalpinia ferrea Mart. (Caesalpiniaceae), foram realizados testes preconizados pela Farmacopéia Brasileira IV e pela Organização Mundial da Saúde para a avaliação da pureza, através das determinações de materiais estranhos, cinzas totais, cinzas insolúveis em ácido, umidade, ensaios-limites de cloretos e sulfatos, além da pesquisa de contaminantes microbianos no material pulverizado. Para a caracterização dos constituintes, eventualmente presentes na droga vegetal, foram realizadas as determinações de taninos, dos índices de espuma, intumescimento e amargor e o teste de hemólise; também foi realizada a caracterização do extrato etanólico bruto e suas frações por cromatografia em camada delgada.Unitermos: Caesalpinia ferrea; controle de qualidade; drogas vegetais ABSTRACT: Physico-chemical and biological characterization of Caesalpinia ferrea Mart. stems. In order to establish parameters for the control quality of Caesalpinia ferrea Mart. stem, the drug was characterized by the methods described in Farmacopéia Brasileira IV and by the World Health Organization. The powdered plant material was submitted to the following tests for purity evaluation: foreign matter, total ash, acid insoluble ash, water content, chloride and sulfate limit assays, and microbiological contamination. Determination of haemolytic activity, foaming index, swelling index and bitterness index were done to characterize the constituents eventually present in the drug, as well as the characterization of the ethanolic crude extract and its fractions by thinlayer chromatography.

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Pharmakognosie — Phytopharmazie

Cited by 150 publications

References 0 publications

Saponins increase susceptibility of vancomycin-resistant enterococci to antibiotic compounds

Saponins increase susceptibility of vancomycin-resistant enterococci to antibiotic compounds

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

Caracterização físico-química e biológica do caule de Caesalpinia ferrea Mart.

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