Phase behavior of the DOPE + DOPC + alkanol system

Klacsová, Mária; Karlovská, Janka; Uhrı́ková, Daniela; Funari, Sérgio S.; Balgavý, P

doi:10.1039/c4sm00530a

Cited by 5 publications

(4 citation statements)

References 50 publications

(61 reference statements)

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“…16.2 nm −1 , respectively, which are consistent with peaks of the XRD pattern of the powder BUD reported in [42]. A similar effect was previously observed and reported, for example, for sitosterol in the egg yolk phosphatidylcholine bilayer [43] or long-chain alcohols in the mixture of dioleoylphosphatidylethanolamine (DOPE)/dioleoylphosphatidylcholine (DOPC) [44]. It should be noted that we did not observe a similar effect for BUD/Curosurf ® in the presence of Ca 2+ (Figure 2A).…”

Section: Saxs/waxs Studysupporting

confidence: 90%

Thermodynamic and Structural Study of Budesonide—Exogenous Lung Surfactant System

Keshavarzi,

Asi Shirazi,

Korfanta

et al. 2024

IJMS

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The clinical benefits of using exogenous pulmonary surfactant (EPS) as a carrier of budesonide (BUD), a non-halogenated corticosteroid with a broad anti-inflammatory effect, have been established. Using various experimental techniques (differential scanning calorimetry DSC, small- and wide- angle X-ray scattering SAXS/WAXS, small- angle neutron scattering SANS, fluorescence spectroscopy, dynamic light scattering DLS, and zeta potential), we investigated the effect of BUD on the thermodynamics and structure of the clinically used EPS, Curosurf®. We show that BUD facilitates the Curosurf® phase transition from the gel to the fluid state, resulting in a decrease in the temperature of the main phase transition (Tm) and enthalpy (ΔH). The morphology of the Curosurf® dispersion is maintained for BUD < 10 wt% of the Curosurf® mass; BUD slightly increases the repeat distance d of the fluid lamellar phase in multilamellar vesicles (MLVs) resulting from the thickening of the lipid bilayer. The bilayer thickening (~0.23 nm) was derived from SANS data. The presence of ~2 mmol/L of Ca2+ maintains the effect and structure of the MLVs. The changes in the lateral pressure of the Curosurf® bilayer revealed that the intercalated BUD between the acyl chains of the surfactant’s lipid molecules resides deeper in the hydrophobic region when its content exceeds ~6 wt%. Our studies support the concept of a combined therapy utilising budesonide—enriched Curosurf®.

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Section: Saxs/waxs Studysupporting

confidence: 90%

Thermodynamic and Structural Study of Budesonide—Exogenous Lung Surfactant System

Keshavarzi,

Asi Shirazi,

Korfanta

et al. 2024

IJMS

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“…Q10 strikingly lowered T h , which is similar to the effect of alkanes on fully hydrated DEPE dispersions (Chen and Rand 1998) or the effect of alkanols on fully hydrated dispersions of mixtures of DOPE and DOPC (Klacsová et al 2014). Similar to other hydrophobic substances, Q10 increases the chain volume term of the packing parameter due to its presence in the chain region of the bilayers (Gomez-Fernandez et al 1999).…”

Section: Discussionmentioning

confidence: 52%

“…If this effect is bigger than the effect of filling the interstices, then a smaller H II lattice parameter is measured. Fully hydrated dispersions of mixtures of DOPE and DOPC containing alkanols, which contain a hydroxyl group like the headgroup of UH, showed such a decrease of the H II lattice parameter (Klacsová et al 2014). The authors similarly argue that the alkanols interact with the headgroup region of the monolayers of the H II lattice.…”

Section: Discussionmentioning

confidence: 91%

“…We made the assignment of the reflections to the phases by comparison with the diffraction patterns presented in (Wang and Quinn 2002). The lattice parameter of the L β phase and the lattice parameter of the L α phase d could be calculated out of the position of the (1) reflection s(1) by d = 1/s(1) (Klacsová et al 2014). The H II lattice parameter a could be calculated out of the (1,0) reflection of the H II phase s(1,0) by a = 2/( √ 3 · s(1, 0)).…”

Section: Pure Fully Hydrated Pope Dispersionsmentioning

confidence: 99%

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The location of coenzyme Q10 in phospholipid membranes made of POPE: a small-angle synchrotron X-ray diffraction study

2015

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The location of coenzyme Q10 (Q10) inside the inner mitochondrial membrane is a topic of research aiming at a deeper understanding of the function of the mitochondrial respiratory chain. We investigated the location of Q10 inside model membranes made of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine by means of small-angle synchrotron X-ray diffraction. Q10, which stands for ubiquinone-10 (UQ) or ubihydroquinone-10 (UH), did not remarkably influence the main phase transition temperature, but significantly decreased the lamellar-inverse hexagonal phase transition temperature (T(h)). The effect of UH on T(h) was stronger than the effect of UQ and the effect of liquid Q10 on T(h) was stronger than the effect of crystalline Q10. In the presence of Q10, the lattice parameters of the lamellar phases remained unchanged, whereas the H II lattice parameter was clearly influenced: While UQ had an increasing effect, UH had a decreasing effect. Furthermore, Q10 prevented the formation of cubic phases. The results give new evidence that the headgroup of Q10 is distant from the center of the membrane, which might be important for the function of the mitochondrial respiratory chain.

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Structural changes of a sodium dodecyl sulfate (SDS) micelle induced by alcohol molecules

Méndez-Bermúdez

Domínguez

2016

J Mol Model

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Coarse-grained dynamical simulations have been performed to investigate the behavior of a surfactant micelle in the presence of six different alcohols: hexanol, octanol, decanol, dodecanol, tetradecanol, and hexadecanol. The self-assembly of sodium dodecyl sulfate (SDS) is modified by the alcohol molecules into cylindrical and bilayer micelles as a function of the alcohol/SDS mass ratio. Therefore, in order to understand, from a molecular point of view, how SDS and alcohol molecules self-organize to form the new micelles, different studies were carried out. Analysis of micelle structures, density profiles, and parameters of order were conducted to characterize the shape and size of those micelles. The density profiles revealed that the alcohol molecules were located at the water-micelle interface next to the SDS molecules at low alcohol/SDS mass ratio. At high alcohol/SDS mass ratios, alcohol molecules moved to the middle of the micelle by increasing their size and by producing a structural change. Moreover, micelle structures and sizes were influenced not only by the alcohol/SDS mass ratio but also by the order of the SDS and alcohol tails. Finally, the size of the micelles and enthalpy calculations were used as order parameters to determine a structural phase diagram of alcohol/SDS mixtures in water. Graphical Abstract Structural transition of SDS/alcohol mixtures.

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Phase behavior of the DOPE + DOPC + alkanol system

Cited by 5 publications

References 50 publications

Thermodynamic and Structural Study of Budesonide—Exogenous Lung Surfactant System

Thermodynamic and Structural Study of Budesonide—Exogenous Lung Surfactant System

The location of coenzyme Q10 in phospholipid membranes made of POPE: a small-angle synchrotron X-ray diffraction study

Structural changes of a sodium dodecyl sulfate (SDS) micelle induced by alcohol molecules

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