1993
DOI: 10.1006/abio.1993.1510
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Phase-Modulation Fluorescence Lifetime Immunoassay of Dichlorprop

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Cited by 6 publications
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“…Although fluorescence lifetime is a concentration-independent property of the excited state (it depends on structure, configuration and microenvironment), quantitative results can be obtained from fractional contributions of the components to the total intensity of the mixture. Phase-modulation fluorescence lifetime measurements have been used to resolve several analytical problems, including multicomponent analysis [1][2][3][4][5]. However, to the best of our knowledge, stereoisomers purity * Corresponding author.…”
Section: Introductionmentioning
confidence: 99%
“…Although fluorescence lifetime is a concentration-independent property of the excited state (it depends on structure, configuration and microenvironment), quantitative results can be obtained from fractional contributions of the components to the total intensity of the mixture. Phase-modulation fluorescence lifetime measurements have been used to resolve several analytical problems, including multicomponent analysis [1][2][3][4][5]. However, to the best of our knowledge, stereoisomers purity * Corresponding author.…”
Section: Introductionmentioning
confidence: 99%
“…-Imagem é obtida a partir do tempo de vida de fluorescência ao invés da intensidade de fluorescência -Menos dano à amostra e contaminação -Imagens de alta resolução em espécies vivas Imunoensaios por fluorescência 31,32 Década de 1970 -Baseados na utilização de anticorpos fluorescentes -Alternativa segura a ensaios com radioisótopos -Existem ensaios baseados em anistoropia, 33 tempo de vida 34 e FRET 35 Citometria de fluxo 36 Década de 1970 -Análise de suspensões de células baseada na fluorescência -Muito utilizada na análise de células sanguíneas PCR em tempo real 37 Fim do século XX -Adaptação da técnica de PCR utilizando sondas fluorescentes para análises quantitativas…”
Section: Fluorescência: Teoria E Aplicabilidade Analíticaunclassified