Phase Partitioning of Biomolecules: Solubilities of Amino Acids

Chen, Chau‐Chyun; Zhu, Yizu; Evans, Lawrence B.

doi:10.1002/btpr.5420050309

Cited by 117 publications

(95 citation statements)

References 13 publications

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“…The number of estimated parameters varies from 3 to 10. Chen et al (1989) added two different contributions for the calculation of the excess Gibbs energy of the system: one is the result of the long-range interactions and was represented by a Pitzer-DebyeHuckel term (Pitzer, 1980); the other is due to local interactions and was formulated by a modified form of the NRTL equation (Renon and Prausnitz, 1968) with two adjustable energy parameters for each amino acidJwater pair. As the data were correlated, each amino acid separately, the results were very satisfactory, both for correlation of activity coefficients (mean deviation of 2 % ) and of solubilities (mean deviation < 1.8%).…”

Section: Introductionmentioning

confidence: 99%

Solubility of Amino Acids: A Group-Contribution Model Involving Phase and Chemical Equilibria

Pinho¹,

Silva²,

Macedo³

1994

Ind. Eng. Chem. Res.

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A new model is proposed to represent the solubility behavior of 14 amino acids and 5 small peptides in water. The UNIFAC model is combined with a Debye-Huckel term to describe the activity coefficients of the species present in the biomolecule/water system. New groups have been defined according to the group-contribution concept, and chemical equilibrium is taken into account simultaneously with the physical equilibrium. To estimate the new interaction parameters, molal activity coefficient data from the literature were used. These parameters, in addition to solubility data, were the basis for the correlation of the solubility product of the amino acids. Using this approach, satisfactory results were obtained in the representation and prediction of the solubilities of amino acids in aqueous solutions at different conditions of temperature and pH.

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Section: Introductionmentioning

confidence: 99%

Solubility of Amino Acids: A Group-Contribution Model Involving Phase and Chemical Equilibria

Pinho¹,

Silva²,

Macedo³

1994

Ind. Eng. Chem. Res.

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“…Meanwhile, chemical analyses suggest that the protein-like fluorophores consist primarily of proteinaceous materials (e.g., proteins and peptides; Yamashita and Tanoue, 2003;Maie et al, 2007), and this DOC fraction is generally hydrophilic and low molecular weight (LMW) compounds (e.g., Sommerville and Preston, 2001). Results of Chen et al (1989) and Fuchs et al (2006) showed that solubility of the proteinaceous materials in LMW is generally affected by neither pH within the normal range of 6-9 nor colloid coagulation. Therefore, increasing ionic strength (or salinization) can enhance the solubility of the proteinaceous materials via sodium dispersion (Green et al, 2008(Green et al, , 2009a or through nonspecific electrostatic interactions at low salinities (called a "salting-in" effect) (Tanford, 1961;Chen et al, 1989).…”

Section: S Duan and S S Kaushal: Salinization Alters Fluxes Of Biomentioning

confidence: 96%

“…Results of Chen et al (1989) and Fuchs et al (2006) showed that solubility of the proteinaceous materials in LMW is generally affected by neither pH within the normal range of 6-9 nor colloid coagulation. Therefore, increasing ionic strength (or salinization) can enhance the solubility of the proteinaceous materials via sodium dispersion (Green et al, 2008(Green et al, , 2009a or through nonspecific electrostatic interactions at low salinities (called a "salting-in" effect) (Tanford, 1961;Chen et al, 1989). Furthermore, because stream sediments are generally enriched in these labile, proteinaceous materials derived from biofilms (algae and microbes) and wastewater organics in urban watersheds (Daniel et al, 2002;Kaushal et al, 2011Kaushal et al, , 2014bKaushal and Belt, 2012;Newcomer et al, 2012;, it is reasonable that salinization can mobilize a large amount of protein-like labile dissolved organic matter from stream sediments.…”

Section: S Duan and S S Kaushal: Salinization Alters Fluxes Of Biomentioning

confidence: 96%

Salinization alters fluxes of bioreactive elements from stream ecosystems across land use

Duan

Kaushal

2015

Biogeosciences

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Abstract. There has been increased salinization of fresh water over decades due to the use of road salt deicers, wastewater discharges, saltwater intrusion, human-accelerated weathering, and groundwater irrigation. Salinization can mobilize bioreactive elements (carbon, nitrogen, phosphorus, sulfur) chemically via ion exchange and/or biologically via influencing of microbial activity. However, the effects of salinization on coupled biogeochemical cycles are still not well understood. We investigated potential impacts of increased salinization on fluxes of bioreactive elements from stream ecosystems (sediments and riparian soils) to overlying stream water and evaluated the implications of percent urban land use on salinization effects. Two-day incubations of sediments and soils with stream and deionized water across three salt levels were conducted at eight routine monitoring stations across a land-use gradient at the Baltimore Ecosystem Study Long-Term Ecological Research (LTER) site in the Chesapeake Bay watershed. Results indicated (1) salinization typically increased sediment releases of labile dissolved organic carbon (DOC), dissolved inorganic carbon (DIC), total dissolved Kjeldahl nitrogen (TKN) (ammonium + ammonia + dissolved organic nitrogen), and sediment transformations of nitrate; (2) salinization generally decreased DOC aromaticity and fluxes of soluble reactive phosphorus from both sediments and soils; (3) the effects of increased salinization on sediment releases of DOC and TKN and DOC quality increased with percentage watershed urbanization. Biogeochemical responses to salinization varied between sediments and riparian soils in releases of DOC and DIC, and nitrate transformations. The differential responses of riparian soils and sediments to increased salinization were likely due to differences in organic matter sources and composition. Our results suggest that short-term increases in salinization can cause releases of significant amounts of labile organic carbon and nitrogen from stream substrates and organic transformations of nitrogen and phosphorus in urban watersheds. Given that salinization of fresh water will increase in the future due to human activities, significant impacts on carbon and nutrient mobilization and water quality should be anticipated.

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“…There are two main groups of models: (i) those which consider that the amino acid dissociation gives rise to charged species in solution, and take account of long-range forces using a Debye-Hückel term (UNIQUAC model by Peres and Macedo [24], UNIFAC group-contribution method by Pinho et al [25]); (ii) those which do not consider the long-range interactions, as the amino acid in pure water is mainly in zwitterionic form {nonrandom two-liquid (NRTL) equation by Chen et al [26], UNIFAC method by Kuramochi [27], modified Wilson equation by Xu et al [28]}. The results obtained are all very satisfactory, being the modified Wilson equation [28] and the UNIQUAC method E. A. MACEDO [24] those giving the better predictions.…”

Section: Modelingmentioning

confidence: 99%

Solubility of amino acids, sugars, and proteins

Macedo

2005

Pure and Applied Chemistry

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Abstract:The importance of biomolecules is well recognized nowadays, owing to their application in many industrial processes, particularly in the food, pharmaceutical, and cosmetic industries.Amino acids, carbohydrates, and proteins are the three types of biomolecules considered in this review. Solubilities of several amino acids and sugars have been measured in water and, more recently, in mixed solvents, both with or without salts. Experimental data on partition of proteins in aqueous two-phase systems (ATPSs), with polymers or salts, have also been reported in the literature. The experimental techniques used are briefly discussed. Regarding modeling, the complexity of these solutions is very high. Sugars form strong hydrogen bonds with one another and with water or other solvents like alcohols. Liquid solutions with amino acids or proteins present not only short-range interaction forces, but also long-range forces, owing to the appearance of charged species. The capabilities of different molecular models and group-contribution-based methods are shown.

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Phase Partitioning of Biomolecules: Solubilities of Amino Acids

Cited by 117 publications

References 13 publications

Solubility of Amino Acids: A Group-Contribution Model Involving Phase and Chemical Equilibria

Solubility of Amino Acids: A Group-Contribution Model Involving Phase and Chemical Equilibria

Salinization alters fluxes of bioreactive elements from stream ecosystems across land use

Solubility of amino acids, sugars, and proteins

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