Phase-Specific Protein Expression in the Dimorphic YeastSaccharomyces cerevisiae

Viard, B.; Kuriyama, Hiroshi

doi:10.1006/bbrc.1997.6461

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…Many fungi also switch their patterns of metabolic conversions, gene expression and protein secretion (55,56). Yeast form and filamentous cells also differ in their expression and accumulation of undesirable cell constituents and the ease with which cells can be disrupted or fractionated (15).…”

Section: Considerations and Applicationsmentioning

confidence: 99%

Filamentous growth in Saccharomyces cerevisiae

Ceccato-Antonini¹,

Sudbery²

2004

Braz. J. Microbiol.

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Fungal dimorphism is a complex phenomenon triggered by a large variety of environmental factors and consists of a reversible alternating pattern of growth between different elliptical and filamentous forms of cells. Understanding the mechanisms that regulate these events is of major interest because of their implications in fungal pathogenesis, cell differentiation and industry. Diploid cells of Saccharomyces cerevisiae transform from budding yeast to pseudohyphae when starved for nitrogen, giving the cells an advantage in food foraging, which is sensed by at least two signal transduction pathways: the MAP kinase (MAPK) and the PKA (cAMP-dependent protein kinase A) pathways. The output of these signalling pathways is the expression of pseudohypha-specific genes, whose expression profiles change and is accompanied by a G2 delay in the cell cycle and a prolonged period of polarized growth. Haploid yeast strains show a similar growth type after prolonged incubation on rich medium plates. The cells form chains and invade the agar on the edge of the colony, but they do not become elongated. This growth type is referred to as haploid invasive growth. Alcohols can also induce filamentous growth in S. cerevisiae, promoting aberrant and elongated morphology. The three forms of filamentous growth are revised in this article and also the pathways involved in sensing, signaling and signal transduction during filamentous growth.

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Section: Considerations and Applicationsmentioning

confidence: 99%

Filamentous growth in Saccharomyces cerevisiae

Ceccato-Antonini¹,

Sudbery²

2004

Braz. J. Microbiol.

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“…It requires the integration of nutritional signals with the co-ordinated expression of a large number of genes involved in diverse cellular processes such as the cell cycle, budding, flocculation and cell wall maintenance (Cid et al 1995). A group of nine proteins was highly over-expressed during the yeast phase when another group of 12 was under-expressed, this phenomenon being reversed during the pseudohyphal phase (Viard and Kuriyama 1997). These results suggest that dimorphism in S. cerevisiae is associated with the expression of specific proteins.…”

Section: Introductionmentioning

confidence: 79%

Biotechnological implications of filamentation in Saccharomyces cerevisiae

Ceccato-Antonini

2008

Biotechnol Lett

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The genetics governing the morphological switch from round or ovoid cells to filamentous growth in Saccharomyces cerevisiae has received significant interest in relation to sensing and signaling pathways as well as the control of cell processes including budding, elongation and adhesion. Little is known about the environmental signals which trigger these morphological changes from a biotechnological point of view. This review aims to highlight the main causes of filamentous growth in S. cerevisiae in its industrial setting with the purpose of stimulating additional studies within this field.

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“…Four of these ( Aspergillus , Fusarium , Magnaporthe and Neurospora ) are classified as filamentous fungi, while the fifth, Saccharomyces , has a dimorphic behaviour, exhibiting both yeast forms and pseudohyphal phases. Dimorphism in S. cerevisiae is associated with the expression of specific proteins [25]. Saccharomyces EST sequences were considered in the ontologies analysis, to investigate for a possible differential distribution of ontologies occurrences with respect to the other monomorphic filamentous fungi.…”

Section: Utility and Discussionmentioning

confidence: 99%

ESTuber db: an online database for Tuber borchii EST sequences

et al. 2007

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BackgroundThe ESTuber database () includes 3,271 Tuber borchii expressed sequence tags (EST). The dataset consists of 2,389 sequences from an in-house prepared cDNA library from truffle vegetative hyphae, and 882 sequences downloaded from GenBank and representing four libraries from white truffle mycelia and ascocarps at different developmental stages. An automated pipeline was prepared to process EST sequences using public software integrated by in-house developed Perl scripts. Data were collected in a MySQL database, which can be queried via a php-based web interface.ResultsSequences included in the ESTuber db were clustered and annotated against three databases: the GenBank nr database, the UniProtKB database and a third in-house prepared database of fungi genomic sequences. An algorithm was implemented to infer statistical classification among Gene Ontology categories from the ontology occurrences deduced from the annotation procedure against the UniProtKB database. Ontologies were also deduced from the annotation of more than 130,000 EST sequences from five filamentous fungi, for intra-species comparison purposes.Further analyses were performed on the ESTuber db dataset, including tandem repeats search and comparison of the putative protein dataset inferred from the EST sequences to the PROSITE database for protein patterns identification. All the analyses were performed both on the complete sequence dataset and on the contig consensus sequences generated by the EST assembly procedure.ConclusionThe resulting web site is a resource of data and links related to truffle expressed genes. The Sequence Report and Contig Report pages are the web interface core structures which, together with the Text search utility and the Blast utility, allow easy access to the data stored in the database.

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Phase-Specific Protein Expression in the Dimorphic YeastSaccharomyces cerevisiae

Cited by 7 publications

References 21 publications

Filamentous growth in Saccharomyces cerevisiae

Filamentous growth in Saccharomyces cerevisiae

Biotechnological implications of filamentation in Saccharomyces cerevisiae

ESTuber db: an online database for Tuber borchii EST sequences

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