Phase transfer of fatty acids into ultrasmall nanospheres for colorimetric detection of lipase and albumin

Guo, Chao; Zhai, Jingying; Chen, Qinghan; Du, Xinfeng; Xie, Xiaojiang

doi:10.1039/d2cc01089e

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…The triglycerides in the substrate are hydrolyzed by lipase to release the fatty acids and enter the small nanospheres to protonate the CHI. This process is also reversible, i.e., CHI can be deprotonated again to appear red, and the detection can be captured by the naked eye or a camera [225]. Wang et al used Tween 20 as a stabilizer to avoid the clustering of AuNPs.…”

Section: Optical Methodsmentioning

confidence: 99%

“…Fatty acids as ion exchangers 1.8 µg/mL \ Human-eye readable [225] Altered LC molecular arrangement of lipid molecules 1 nM 2-10 nM Small fiber optic sensors [233] Hydrolysis of polymer changes the conductive area of electrodes 8 mU/mL 0.0099-1.68 U/mL Miniaturized equipment [236] Hydrolysis of polymer changes the thickness of capacitors \ 0.0073-3.9 U/mL Miniaturized equipment [237] Viscosity change 0.052 U/mL 0.052-30 U/mL Paper based [242] Hydrolysis of 4-acetaminophen acetate \ \ Blood glucose meter [243] Hydrolysis releases H + ions to improve electrical conductivity 6.087 U/L \ Paper based [244]…”

Section: Hydrolysismentioning

confidence: 99%

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Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Yin,

Cui,

Yang

et al. 2023

Chemosensors

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This paper is a comprehensive review of the techniques for the detection of pancreatic enzymes, which are common biochemical indicators of pancreatitis, including amylase, trypsin, chymotrypsin, elastase, and lipase. Pancreatitis is a disease with self-digestion due to the abnormal activation of digestive enzymes in the pancreas. Hospitalization is often required due to the lack of convenient therapeutic agents. The main recent results are reported in this review, especially the techniques that enable portability and Point-of-Care testing (POCT). This is because timely diagnosis at the early stage and avoiding recurrence after recovery are the keys to treatment. It is also important to reduce the rate of misdiagnosis and to avoid overtreatment. Various detection methods are discussed, with particular attention given to the implementation of chemical sensing and probe design. The new sensing technology for digestive enzymes makes it possible to perform early screening for pancreatitis in remote areas or in one’s own home.

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Section: Optical Methodsmentioning

confidence: 99%

Section: Hydrolysismentioning

confidence: 99%

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Yin,

Cui,

Yang

et al. 2023

Chemosensors

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“…( 9) Developing appropriate sample preparation protocols and evaluating any potential matrix effects. (10) Exploring the application of the method in clinical settings for therapeutic drug monitoring and pharmacokinetic studies. (11) Measuring drug concentrations in patient samples to optimize dosing regimens and improve outcomes.…”

Section: Analysis Of Biological and Pharmaceutical Samplesmentioning

confidence: 99%

“…The cleating agent (ionophore) in the nanospheres selectively binds the analyte ions, leading to changes in the optical, electrical, or mechanical properties of the nanospheres that can be detected and quantified. Recently, nanosphere emulsions containing a complexing agent and ion exchanger have been used in the extraction of target analytes (ions) in complex samples [2,[7][8][9][10]. The incorporation of the ion exchanger in the nanosphere emulsion can increase the sensitivity of the emulsion as it allows its separation from other interfering species in the sample solution and facilitates the binding of the target analyte.…”

Section: Introductionmentioning

confidence: 99%

Ionophore-Based Nanosphere Emulsion Incorporating Ion-Exchanger for Picogram Potentiometric Determination of HCV Drug (Daclatasvir) in Pharmaceutical Formulations and Body Fluids

et al. 2023

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Daclatasvir dihydrochloride (DAC) is a drug used to treat hepatitis C virus (HCV) infection. In this study, an ionophore-based nanosphere emulsion was made of tricresyl phosphate (TCP) as the oil phase that is dispersed in water using Pluronic F-127 as an emulsifying agent. The nanospheres, consisting of the oil phase TCP, were doped with sodium tetraphenyl borate (Na-TPB) as a cation-exchanger and dibenzo-18-Crown-6 (DB18C6) as an ionophore (chelating agent) for DAC. The nanosphere emulsion was employed as a titrant in the complexometric titration of DAC (the analyte), and the DAC-selective electrode (ISE) was used as an indicator electrode to detect the endpoint. In the sample solution, DAC2+ ions diffused into the emulsified nanospheres, replaced Na+ from the ion exchanger (Na-TPB), and bonded to the ionophore (DB18C6). The DAC-selective nanospheres were successfully utilized to determine DAC in various samples, including standard solutions, commercial tablets (Daclavirocyrl®), serum, and urine. The method exhibited a linear dynamic range of 81.18 µg/mL to 81.18 pg/mL (10−4 to 10−10 M), achieved high recovery values ranging from 99.4% to 106.5%, and displayed excellent selectivity over similar interfering species (sofosbuvir and ledipasvir). The proposed method offers a new approach to determine the drug species (neutral, anionic, and cationic) without the requirement of water-soluble ligands or pH control.

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A Tunable Colorimetric Carbon Dioxide Sensor Based on Ion‐Exchanger‐ and Chromoionophore‐Doped Hydrogel

Zhang

Zhai

et al. 2023

Analysis & Sensing

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We report here a colorimetric carbon dioxide (CO2) optode sensor with a polypropylene microporous membrane on top of a thin layer (30 μm) of polyurethane hydrogel. The diffusion of CO2 across the polypropylene membrane induced pH changes in the hydrogel containing a lipophilic indicator, a cation exchanger, and a cationic amine. The ratio of the indicator and the cation exchanger was successfully utilized to adjust the sensitivity of the CO2 response. Increasing the relative amount of the cation exchanger made the sensor much more sensitive to CO2 in the lower concentration range. Moreover, the carbarmic formation reaction between the primary amine and CO2 was investigated. The results indicated a very small contribution of carbarmic formation to the overall pH change. With a detection limit of 0.014 % for the CO2 partial pressure, the sensor was successful applied to monitor CO2 evolution during yeast catalyzed flour fermentation.

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Phase transfer of fatty acids into ultrasmall nanospheres for colorimetric detection of lipase and albumin

Abstract: Colorimetric detection of fatty acids during biological interactions is extremely difficult since they are optically silent. Here, fatty acids are found to function as ion-exchanger in ultrasmall polymeric nanospheres to...

Cited by 4 publications

References 31 publications

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Ionophore-Based Nanosphere Emulsion Incorporating Ion-Exchanger for Picogram Potentiometric Determination of HCV Drug (Daclatasvir) in Pharmaceutical Formulations and Body Fluids

A Tunable Colorimetric Carbon Dioxide Sensor Based on Ion‐Exchanger‐ and Chromoionophore‐Doped Hydrogel

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