Phase transitions and mechanisms of cryoprotection of serum-/xeno-free media based on dextran and dimethyl sulfoxide

Photomechanical response under physiological conditions of azobenzene-containing 4D-printed liquid crystal elastomer actuators

“…Density and thermal properties (specific heat capacity: 4000 J kg À1 1C at 25 1C; thermal conductivity: 0.58 W m À1 1C at 25 1C) are very similar to those of water. 40,41 The samples are fully immersed in the fluid to ensure a good thermal contact with the fluid also avoiding effects of the air-liquid interface. 42 In order to have data comparable to those of the photomechanical experiments (see the next section), the uniaxially oriented LCE elements were fixed at one extreme and a weight of 1 g was attached to their lower ending.…”

Open‐source hardware‐ and software‐based cryomicroscopy system for investigation of phase transitions in cryobiological research

“…Our previous work has shown that HES-and, especially, PEG-containing compositions had lower ability to preserve ICs compared to Dex-containing ones. 16 Thus we speculated that the observed physical processes should be different in these media and correlate with the assessed cell recovery. The data of the present work has confirmed this statement.…”

“…These combinations of Me 2 SO and polymers proved to be optimal for cryopreservation of ICs. 16 In some experiments, 1.4 M Me 2 SO was used. In this case, they were referred to as 1.4 Me 2 SO+Dex, 1.4 Me 2 SO+HES, 1.4 Me 2 SO+PEG.…”

“…[7][8][9][10][11][12][13][14][15] In our previous work, some serum-free compositions able to preserve ICs were investigated by differential scanning calorimetry and thermomechanical analysis. 16 These serum-free compositions included a combination of dimethyl sulphoxide (Me 2 SO) and one of the polymers: dextran (Dex), hydroxyethyl starch (HES) or polyethylene glycol (PEG). In this previous work, Ham's F12 medium supplemented with 0.7 M Me 2 SO and 100 mg/mL of Dex (0.7 Me 2 SO+Dex) had an exceptional property in preserving ICs of rat testis.…”

“…Note that changing concentration of Me 2 SO, FBS or Dex in these compositions did not improve the outcome. 16 The above-mentioned polymers are common nonpenetrating extracellular cryoprotective agents. [20][21][22][23] Dextran and HES have been used as plasma volume expanders and as antithrombotic agents in clinical practice at a concentration of about 100 mg/mL.…”