Phase Transitions, Cholesterol and Raft Structures in Films and Bilayers of a Natural Membranous System

Nag, Kaushik; Fritzen‐Garcia, Mauricia; Devraj, Ravi; Hillier, Ashley; Rose, Doyle

doi:10.1002/9780470388495.ch15

Cited by 3 publications

(3 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various spectroscopic techniques are used to measure motion of lipids and can detect the gel to liquid crystalline transition temperature (Mouritsen & Bloom, 1993). In this study, Raman spectroscopy, a non‐perturbing technique that provides a snapshot of membrane structure on the order of 10 −14 s, was used (Nag et al, 2008). The parameter examined in this study, the CH 2 symmetric stretching frequency, provides information on conformational order of constituent lipids in membranes.…”

Section: Discussionmentioning

confidence: 99%

Diet and temperature affect liver lipids and membrane properties in steelhead trout ( Oncorhynchus mykiss )

et al. 2021

Self Cite

View full text Add to dashboard Cite

Fish regulate cellular membrane fluidity in response to temperature by restructuring membrane lipid composition. This study evaluated the effect of diet and temperature on liver membranes in steelhead trout, an important aquaculture species. Oncorhynchus mykiss were fed three commercial diets with different levels of PUFA (lower (L‐n3); medium (M‐n3); higher (H‐n3) omega 3) from marine, terrestrial, and vegetable oils; the effect of temperature changes on liver membrane was measured. Fish fed the H‐n3 diet had the most linear response in Raman spectroscopy, indicating that they can adapt to changes in temperature with the least effect on liver membrane, due to the higher polyunsaturate:saturate ratio in the diet, counteracting the influence of low temperature. L‐n3‐fed fish presented increased membrane fluidity at all temperatures, highlighting the influence of terrestrial fatty acids on membrane properties. These results underscore changes in sterol:phospholipid ratios as a key response for membrane adaptability to environmental changes, and the necessity to include environmental variables when testing new diets. Substitution of fish oil with vegetable oils may compromise sterol:phospholipid ratios, affecting membrane adaptability. This study shows changes at cellular level in liver tissue for fish fed different diets and subjected to different water temperatures.

show abstract

Section: Discussionmentioning

confidence: 99%

Diet and temperature affect liver lipids and membrane properties in steelhead trout ( Oncorhynchus mykiss )

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Various previous studies have used the method to study lipid films as well as those with serum proteins 7,11,15,16,27 . Our study examined the effects of serum or serum components in the formation of BLES domain.…”

Section: Structure and Dysfunctionmentioning

confidence: 99%

Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

et al. 2014

Self Cite

View full text Add to dashboard Cite

“…Lipid monolayers and bilayers receive a great deal of attention because of their important biological role as a principle component of biological membranes. Nowadays, these lipid layers are used as simple model systems to investigate the physicochemical properties of biological membranes . These systems are known to be self-aggregating and are driven by hydrophobic effects and headgroup interactions between the lipid molecules, resulting in mesoscopic domain structures with a variety of morphologies.…”

Section: Introductionmentioning

confidence: 99%

Heterochiral Domain Formation in Homochiral α-Dipalmitoylphosphatidylcholine (DPPC) Langmuir Monolayers at the Air/Water Interface

Liu

Guo

2012

J. Phys. Chem. C

View full text Add to dashboard Cite

α-Dipalmitoylphosphatidylcholine (DPPC) Langmuir monolayers at the air/water interface are an important biomimetic system. Previous studies using optical imaging have shown that enantiomerically pure DPPC molecules form periodical homochiral domains with close packing. However, it is unclear whether the chirality of these domains is stable or dynamic. Recently, we investigated the chirality of L-DPPC Langmuir monolayers using secondharmonic-generation linear dichroism (SHG-LD). The change in the degree of chiral excess (DCE) and its sign with time indicated that the chirality transformed from homochiral to heterochiral domains, with an associated change in handedness. Here we propose that hydrolysis of L-DPPC is responsible for the formation of the opposite chiral state. On the basis of quantum chemistry calculations and effective pair potential (EPP) theory, we give a molecular explanation for this transformation. These results provide an insight into the lipid monolayers that the chiral state of domains composed of chiral lipids is dynamic.

show abstract

Phase Transitions, Cholesterol and Raft Structures in Films and Bilayers of a Natural Membranous System

Cited by 3 publications

References 63 publications

Diet and temperature affect liver lipids and membrane properties in steelhead trout ( Oncorhynchus mykiss )

Diet and temperature affect liver lipids and membrane properties in steelhead trout ( Oncorhynchus mykiss )

Effect of Serum, Cholesterol and Low Density Lipoprotein on the Functionality and Structure of Lung Surfactant Films

Heterochiral Domain Formation in Homochiral α-Dipalmitoylphosphatidylcholine (DPPC) Langmuir Monolayers at the Air/Water Interface

Contact Info

Product

Resources

About