PHB recovery by hypochlorite digestion of non-PHB biomass

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

“…In chemical-digestion, sodium hypochlorite is used to solubilize non-P3HB biomass thus achieving separation of P3HB content which can be recovered by centrifugation [147]. While the method is simple and effective, P3HB polymers obtained through hypochlorite digestion are generally of lower molecular masses due to the severe polymer degradation [147].…”

Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media

“…Polyhydroxybutyrate (PHB) is a common homopolymer produced by bacteria but the presence of copolymers improves the properties of the biopolymer [2,3]. Sodium hypochlorite is generally used to hydrolyze the cell wall and recovered using solvents [4]. This leads to generation reduction in the molecular size of the polymer.…”

Hypochlorite digestion method for efficient recovery of PHB from Alcaligenes faecalis

“…PHB and PHA have been regarded as an eco-friendly alternative to the synthetic polymers because they are biodegradable and therefore safe for the environment and possess properties similar to that of synthetic polymers currently in use [1][2][3][4]. PHB possesses only R side chains (lack S side chains) and are therefore biodegradable [1,5,6].…”

“…Method of extraction is equally important in getting high recovery yields; different methods used in the recovery cause severe degradation of PHB and therefore decrease in the recovery yield [2,[13][14][15][16]. The rate of degradation can be reduced several folds by digestion of non-PHB cell mass (NPCM) with sodium hypochlorite [15].…”