Phellopterin isolated from Angelica dahurica reduces blood glucose level in diabetic mice

Abstract

Insulin resistance is the critical condition for the development of metabolic syndromes including type II diabetes and heart disease. To investigate the active components of Angelica dahurica root which is known to increase insulin sensitivity, its methanol extract was subfractionated. The ethyl acetate (EtOAc) fraction of the Angelica dahurica root extract significantly promoted adipocyte differentiation in 3T3-L1 preadipocyte cells. Among the three compounds isolated from the EtOAc extract (bergapten (1), imperatorin (2) and phellopterin (3)), phellopterin (3) induced the highest adipocyte differentiation at 25 and 50 μg/mL. In addition, treatment with imperatorin (2) and phellopterin (3) increased the mRNA expression of peroxisome proliferator-activated receptors γ (PPARγ). In diabetic animal model induced by high-fat diets (HFD) and streptozotocin (STZ), administration of phellopterin ((3), 1 mg/kg and 2 mg/kg) significantly reduced the levels of blood glucose, triglycerides and total cholesterol. Taken together, these results indicate that phellopterin (3) enhances adipocytes differentiation in 3T3-L1 preadipocytes, phellopterin (3) significantly prevents HFD/STZ-induced type Ⅱ diabetes. The present study also provides phellopterin (3) may be a valuable therapeutic alternative for enhancing insulin sensitivity through promotion of adipocyte differentiation and by increasing mRNA expression levels of PPARγ, which is a major mediator of insulin sensitivity.

Mining of simple sequence repeats (SSRs) loci and development of novel transferability-across EST-SSR markers from de novo transcriptome assembly of Angelica dahurica

“…dahurica has been used in traditional Chinese medicine [4] because of its anti-proliferative [5], anti-inflammatory [6], anti-depressive [7], anti-oxidative [8], and anti-microbial [9] effects. It has also been applied to regulate hormones [10], decrease blood glucose levels [11], and treat headaches, ulcers, toothaches, abscesses [12], and other diseases. Angelica dahurica root extracts contain furanocoumarin compounds [13], phenolic compounds, and volatile oils, all of which account for the multiple pharmacological effects of this plant species.…”

Anti-Obesity Potential of Ponciri Fructus: Effects of Extracts, Fractions and Compounds on Adipogenesis in 3T3-L1 Preadipocytes

“…β-Sitosterol, a well-known functional food additive, and imperatorin, showed a significant increase in the lipid production at lower doses (Supplementary Figure S18). The increase in fat deposition by imperatorin might be due to an enhanced expression of PPAR-γ [69]. Phellopterin reduces fat deposition dose-dependently, and was significant compared to the control (Figure 6).…”

Antidiabetic effects of a lipophilic extract obtained from flowers of Wisteria sinensis by activating Akt/GLUT4 and Akt/GSK3β

“…To investigate antidiabetic effects and the potential underlying mechanism of WS-PE in vivo, HFD + low-dose STZ-injection induced type 2 diabetic mice were used as the animal model in the present study. These type 2 diabetic mice are known to share major characteristics with human T2DM, including hyperglycemia, body weight loss, IR, and lipid metabolism disorders (29,30). It is well-known that long-term HFD leads to obesity, IR, and hyperinsulinaemia, and then, the subsequent injection of low-dose STZ, a beta cell toxin, would partly damage pancreatic β-cells and induce body weight loss and high levels of FBG (31).…”