Phenanthrene biodegradation by an algal-bacterial consortium in two-phase partitioning bioreactors

Muñoz, Raúl; Guieysse, Benoît; Mattìasson, Bo

doi:10.1007/s00253-003-1231-9

Cited by 137 publications

(50 citation statements)

References 29 publications

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“…Cirigliano and Carman (1984) reported that Candida lipolytica, which showed a low level of emulsification activity on glucose, demonstrated inducible emulsification activity on a number of hydrophobic carbon substrates. In the present study, with the exception of strain C. maltosa-like, the emulsification ability levels of the yeast strains in naphthalene were higher than those of C. lipolytica grown on water-immiscible carbon substrates (0.50 to 0.98 EU mL −1 ), and comparable to those of bacterial strains grown in PAHs (1.6 to 1.7 EU mL −1 ; Muñoz et al 2003). We hypothesized that emulsification activities were induced slowly in the five yeast isolates by naphthalene.…”

Section: Discussioncontrasting

confidence: 51%

Cell surface properties of five polycyclic aromatic compound-degrading yeast strains

Yan-qin

Zhang

Hesham

et al. 2010

Appl Microbiol Biotechnol

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To investigate the effects of physiological properties on polycyclic aromatic compound (PAH) degradation, the surface tension and emulsification activities, and cell surface hydrophobicity of five PAH-degrading yeast isolates were compared to Saccharomyces cerevisiae from cultures grown with glucose, hexadecane, or naphthalene as carbon sources. The cell surface hydrophobicity values for the five yeast strains were significantly higher than for S. cerevisiae for all culture conditions, although these were highest with hexadecane and naphthalene. Strains with higher hydrophobicity showed higher rates of naphthalene and phenanthrene degradation, indicating that increased cell hydrophobicity might be an important strategy in PAH degradation for the five strains. Emulsification activities increased for all five yeast strains with naphthalene culturing, although no relationship existed between emulsification activity and PAH degradation rate. Surface tensions were not markedly reduced with naphthalene culturing.

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Section: Discussioncontrasting

confidence: 51%

Cell surface properties of five polycyclic aromatic compound-degrading yeast strains

Yan-qin

Zhang

Hesham

et al. 2010

Appl Microbiol Biotechnol

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“…The association of PAH-degrading bacteria with eukaryotic phytoplankton may be viewed as a coevolutionary adaptation based on the ability of the phytoplankton to sequester and/or synthesize PAHs. Although photosynthetically enhanced biodegradation of toxic aromatic pollutants has been demonstrated by using artificial combinations of algal-bacterial consortia (60)(61)(62)(63), little is known about this process in natural algal-bacterial assemblages. The association of these organisms may have potentially profound implications for the natural purging of the water column of PAH contaminants and help contribute to the overall health of the marine ecosystem.…”

Section: Resultsmentioning

confidence: 99%

Polycyclovorans algicola gen. nov., sp. nov., an Aromatic-Hydrocarbon-Degrading Marine Bacterium Found Associated with Laboratory Cultures of Marine Phytoplankton

Gutiérrez

Green

Nichols

et al. 2013

Appl Environ Microbiol

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f A strictly aerobic, halotolerant, rod-shaped bacterium, designated strain TG408, was isolated from a laboratory culture of the marine diatom Skeletonema costatum (CCAP1077/1C) by enrichment with polycyclic aromatic hydrocarbons (PAHs) as the sole carbon source. 16S rRNA gene sequence analysis placed this organism within the order Xanthomonadales of the class Gammaproteobacteria. Its closest relatives included representatives of the Hydrocarboniphaga-Nevskia-Sinobacter clade (<92% sequence similarity) in the family Sinobacteraceae. The strain exhibited a narrow nutritional spectrum, preferring to utilize aliphatic and aromatic hydrocarbon compounds and small organic acids. Notably, it displayed versatility in degrading two-and three-ring PAHs. Moreover, catechol 2,3-dioxygenase activity was detected in lysates, indicating that this strain utilizes the metacleavage pathway for aromatic compound degradation. Cells produced surface blebs and contained a single polar flagellum. The predominant isoprenoid quinone of strain TG408 was Q-8, and the dominant fatty acids were C 16:0 , C 16:1 7c, and C 18:1 7c. The G؉C content of the isolate's DNA was 64.3 mol% ؎ 0.34 mol%. On the basis of distinct phenotypic and genotypic characteristics, strain TG408 represents a novel genus and species in the class Gammaproteobacteria for which the name Polycyclovorans algicola gen. nov., sp. nov., is proposed. Quantitative PCR primers targeting the 16S rRNA gene of this strain were developed and used to show that this organism is found associated with other species of marine phytoplankton. Phytoplankton may be a natural biotope in the ocean where new species of hydrocarbon-degrading bacteria await discovery and which contribute significantly to natural remediation processes.

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“…Photosynthetically enhanced biodegradation of toxic aromatic pollutants has been demonstrated using artificial algal-bacterial consortia (5,33,42,53). However, there is little known about these processes in natural algal-bacterial assemblages.…”

Section: Discussionmentioning

confidence: 99%

Porticoccus hydrocarbonoclasticus sp. nov., an Aromatic Hydrocarbon-Degrading Bacterium Identified in Laboratory Cultures of Marine Phytoplankton

Gutiérrez

Nichols

Whitman

et al. 2012

Appl Environ Microbiol

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A marine bacterium, designated strain MCTG13d, was isolated from a laboratory culture of the dinoflagellate Lingulodinium polyedrum CCAP1121/2 by enrichment with polycyclic aromatic hydrocarbons (PAHs) as the sole carbon source. Based on 16S rRNA gene sequence comparisons, the strain was most closely related to Porticoccus litoralis IMCC2115 T (96.5%) and to members of the genera Microbulbifer (91.4 to 93.7%) and Marinimicrobium (90.4 to 92.0%). Phylogenetic trees showed that the strain clustered in a distinct phyletic line in the class Gammaproteobacteria for which P. litoralis is presently the sole cultured representative. The strain was strictly aerobic, rod shaped, Gram negative, and halophilic. Notably, it was able to utilize hydrocarbons as sole sources of carbon and energy, whereas sugars did not serve as growth substrates. The predominant isoprenoid quinone of strain MCTG13d was Q-8, and the dominant fatty acids were C 16:17c , C 18:17c , and C 16:0 . DNA G؉C content for the isolate was 54.9 ؎ 0.42 mol%. Quantitative PCR primers targeting the 16S rRNA gene of this strain showed that this organism was common in other laboratory cultures of marine phytoplankton. On the basis of phenotypic and genotypic characteristics, strain MCTG13d represents a novel species of Porticoccus, for which the name Porticoccus hydrocarbonoclasticus sp. nov. is proposed. The discovery of this highly specialized hydrocarbon-degrading bacterium living in association with marine phytoplankton suggests that phytoplankton represent a previously unrecognized biotope of novel bacterial taxa that degrade hydrocarbons in the ocean.

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Phenanthrene biodegradation by an algal-bacterial consortium in two-phase partitioning bioreactors

Cited by 137 publications

References 29 publications

Cell surface properties of five polycyclic aromatic compound-degrading yeast strains

Cell surface properties of five polycyclic aromatic compound-degrading yeast strains

Polycyclovorans algicola gen. nov., sp. nov., an Aromatic-Hydrocarbon-Degrading Marine Bacterium Found Associated with Laboratory Cultures of Marine Phytoplankton

Porticoccus hydrocarbonoclasticus sp. nov., an Aromatic Hydrocarbon-Degrading Bacterium Identified in Laboratory Cultures of Marine Phytoplankton

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