Phenol Degradation by Immobilized Cells of Arthrobacter citreus

Karigar, Chandrakant S.; Mahesh, Aravind; Nagenahalli, Manjunath; Yun, Dae‐Jin

doi:10.1007/s10532-005-3048-y

Cited by 92 publications

(59 citation statements)

References 51 publications

(37 reference statements)

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“…Both acidic and alkaline pH caused a marked inhibition of the phenol removal efficiency. Similar results were obtained by Karigar et al (2006) for Arthrobacter citreus. This may be due to the effect of pH on the ionization, which in turn affects the metabolic pathway of the organism.…”

Section: Effect Of Phsupporting

confidence: 88%

BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11

Mohanty

Jena

2017

Braz. J. Chem. Eng.

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-In the present study, a pure culture of bacterium (Pseudomonas sp. Strain NBM11) was isolated from the soil sample from a site contaminated with medical wastes and wastewater. The isolated strain can degrade up to 1000 mg/L of phenol completely. It was observed that temperature, pH and initial concentration of phenol play key roles in determining the rate of phenol degradation. The isolated strain exhibited the maximal degradation of the substrate within a range of pH 6.8 to 7.2 and an incubation temperature between 30 ºC and 32 ºC. It was found that by increasing the concentration of phenol, the lag phase gets extended due to the inhibitory nature of phenol. The kinetic parameters such as µmax (maximum specific growth rate), Ks (half-saturation coefficient) and Ki (substrate inhibition constant) were estimated as 0.184 1/h, 7.79 mg/L and 319.24 mg/L, respectively, by fitting the growth kinetics data to the Haldane model of substrate inhibition. The bacterial strain was immobilized in alginate beads and its phenol degradation efficiency was observed to increase many fold. The immobilized cells were found to be used efficiently for seven cycles consecutively without any decrease in their efficiency.

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Section: Effect Of Phsupporting

confidence: 88%

BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11

Mohanty

Jena

2017

Braz. J. Chem. Eng.

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“…Other Cryptococcus isolates degraded phenol in laboratory media (Middelhoven, 1993;Bergauer et al, 2005) and, in one DNA-SIP study, a member of the same order (Tramellales) was detected as a phenol degrader in soil (DeRito and Madsen, 2009). Equally, Arthrobacter and Rhodococcus isolates can degrade phenol Karigar et al, 2006;Martinkova et al, 2009), and members of both genera were detected by DNA-SIP as phenanthrene degraders in soil (Cebron et al, 2011). Another DNA-SIP study with phenol retrieved mainly Gammaproteobacteria from a silt loam soil (Padmanabhan et al, 2003).…”

Section: Discussionmentioning

confidence: 98%

Importance of soil organic matter for the diversity of microorganisms involved in the degradation of organic pollutants

et al. 2014

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Many organic pollutants are readily degradable by microorganisms in soil, but the importance of soil organic matter for their transformation by specific microbial taxa is unknown. In this study, sorption and microbial degradation of phenol and 2,4-dichlorophenol (DCP) were characterized in three soil variants, generated by different long-term fertilization regimes. Compared with a non-fertilized control (NIL), a mineral-fertilized NPK variant showed 19% and a farmyard manure treated FYM variant 46% more soil organic carbon (SOC). Phenol sorption declined with overall increasing SOC because of altered affinities to the clay fraction (soil particles o2 mm in diameter). In contrast, DCP sorption correlated positively with particulate soil organic matter (present in the soil particle fractions of 63-2000 lm). Stable isotope probing identified Rhodococcus, Arthrobacter (both Actinobacteria) and Cryptococcus (Basidiomycota) as the main degraders of phenol. Rhodococcus and Cryptococcus were not affected by SOC, but the participation of Arthrobacter declined in NPK and even more in FYM. 14 C-DCP was hardly metabolized in the NIL variant, more efficiently in FYM and most in NPK. In NPK, Burkholderia was the main degrader and in FYM Variovorax. This study demonstrates a strong effect of SOC on the partitioning of organic pollutants to soil particle size fractions and indicates the profound consequences that this process could have for the diversity of bacteria involved in their degradation.

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“…The exopolysaccharide selection medium was inoculated with 5% (w/v) of the immobilised cells according to the method of Karigar et al (38) and incubated at 35°C for 48 hours. The EPS produced was isolated and purified according to the method of Cerning et al (39) while its quantification was carried out using the method described by Dubois et al (31).…”

Section: Production Of Eps By Immobilised Wild Type and Mutant Strainsmentioning

confidence: 99%

Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk

Adebayo-Tayo¹,

Agidigbi²,

Alao³

2017

TJS

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Phenol Degradation by Immobilized Cells of Arthrobacter citreus

Cited by 92 publications

References 51 publications

BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11

BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11

Importance of soil organic matter for the diversity of microorganisms involved in the degradation of organic pollutants

Comparative influence of immobilization medium and mutation on EPS - production by L. Plantarum MK 02 isolated from fermented milk

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