Phenolic Constituents, Anti-Inflammatory and Antidiabetic Activities of Cyperus laevigatus L.

Abstract

Background: Cyperus species are well known traditional plants and used for several diseases around the world. Aim of the Study: Our study aimed to identification of the phenolic constituents in addition to evaluation of different extracts of Cyperus laevigatus L as antioxidant, antiinflammatory and antidiabetic agents. Materials and Methods: The phenolic constituents were identified using spectroscopic techniques. The antioxidant activity was evaluated using in vitro DPPH assay. Total extract, methanol and EtOAc fractions were evaluated for their antiinflammatory activity using RAW 264.7 macrophages assay. Antidiabetic activity of the total extract was examined biochemically and histopathologically using streptozotocin-induced diabetic rats. Results: A new flavone, chrysoeriol 7-O-β-(6‴-O-acetyl-β-D-glucopyranosyl)-(1→4) glucopyranoside (1), along with seven knowns (2-8) were isolated from Cyperus laevigatus L. The structures of isolated compounds were established depending upon 1D, 2D-NMR and HR-ESI-MS. The MeOH and EtOAc fractions exhibited significant antioxidant activity while the isolated flavonoids exhibited from moderate to weak antioxidant activity. The total extract, MeOH and EtOAc fractions exhibited significant anti-inflammatory activity using LPS-stimulated RAW 264.7 macrophages model by decreasing of NO accumulation by 76-66% and 84-67%, of the original accumulation values with increasing concentrations in comparison with the reference drug, dexamethasone. The total extract exhibited antidiabetic activity in streptozotocin-induced diabetic rats and this effect was manifested by decreasing serum levels of glucose, glucagon and NO. It also increased level of insulin and promoted paraoxonase activity. Conclusion: These results proved that this plant may be multiple sources for medicinal natural drugs especially for anti-inflammatory and antidiabetic.

UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of Cyperus conglomeratus Rottb. Alcoholic Extract

“…Plants belonging to Cyperus genus were reported to encompass various bioactive agents with potential biological effects. We previously reported the anti-inflammatory and gastroprotective activities of different Cyperus species extracts such as C. laevigatus [ 6 ] and C. alternifolius [ 5 ]. In continuation of our efforts for the evaluation of the biological activity of Cyperus extracts, the present study inspected the gastroprotective effects of different doses (25, 50, and 100 mg/kg) of C. conglomeratus above ground alcohol extracts on ethanol-induced gastric lesions in rats.…”

“…The gastroprotective role of flavonoids and phenolic compounds were described via their anti-inflammatory actions [ 6 ], and decreasing of levels of TNF-α [ 5 ]. The role of polyphenolic metabolites as potent antioxidant agents, especially flavonoids, is well reported, especially when increasing the gastroprotection activity of these compounds against lesions by decreasing lipoperoxides levels and increasing glutathione peroxidase enzyme activity.…”

“…Cyperus species are widely used in traditional medicines around the world as emollient to treat suckling mothers inflamed breasts, aromatic stomachic in nervous gastralgia, analgesic, diuretic, carminative, stimulant, colic remedy, anthelmintic, and astringent. The previous biological studies on Cyperus plants indicated their significant effects including antimicrobial (Sharma et al, 2014), anti-inflammatory [ 6 ], hepatoprotective [ 7 ], gastroprotective [ 5 ], anti-malarial [ 8 ], antioxidant [ 9 ], and anti-diabetic [ 10 ] activities. Several classes of compounds were isolated and reported from Cyperus sp.…”

“…Considering the well-reported medicinal uses of Cyperus plants and continuing our search for bioactive products for the treatment of gastric disorders of natural origin [ 5 , 6 , 22 ], we described herein the first metabolites profile of C. conglomeratus alcohol extract via UPLC-qTOF-MS, and its potential gastroprotective activity as monitored using biochemical and histopathological examinations.…”

Middle East Medicinal Plants in the Treatment of Diabetes: A Review

“…The biochemical markers caused a decrease in glucose level and the promotion of serum insulin in the diabetic group treated with C. laevigatus extract, and this can be explained by flavonoid activity. In addition, the histological examination of the pancreas of the extract-treated rats indicated the normal architecture that was attributed to flavonoids’, flavonoid glycosides’ and phenolic acids’ abilities to regenerate β -cells [ 131 ].…”