Phenolic Content and Antioxidant, Antihyperlipidemic, and Antidiabetogenic Effects of Opuntia dillenii Seed Oil

Abstract

Opuntia dillenii (Ker-Gawl.) Haw. is a medicinal plant that is widely used by the Moroccan population to treat many diseases, thanks to its richness in bioactive molecules. This study aims to evaluate the total phenolic content and antioxidant, antihyperlipidemic, and antidiabetogenic activities of O. dillenii seeds oil (ODSO), in vivo. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay and the Folin–Ciocalteu method were applied in this study to determine antioxidant activity and total phenolic content of ODSO, respectively. The antihyperlipidemic effect of the ODSO (2 ml/kg) was evaluated in the high-fat diet-fed albino mice, relying on lipid profile, blood glucose, and growth performance variations. Moreover, the preventive effect of ODSO was evaluated against alloxan monohydrate-induced diabetes in albino mice. ODSO had the highest total phenolic content (518.18 ± 14.36 mg EAC/kg) and DPPH scavenging activity (IC50 = 0.38 ± 0.08 mg/mL). Furthermore, ODSO showed a significant antidiabetogenic effect by reducing bodyweight loss, blood sugar level rise, and mortality rate caused by alloxan in albino mice. Then, ODSO has exhibited a significant antihyperlipidemic effect by improving the lipid profile disorder and glucose level rise in the blood, produced by the high-fat diet-fed albino mice. Results suggest that antidiabetogenic and antihyperlipidemic activities of ODSO correlate to the phenolic content and antioxidant activity of this oil. Hence, this plant could be a significant source of medically important critical compounds.

Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

“…The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) scavenging activity was measured according to the method described by Bouhrim et al (2020) 31 , with some modifications. Briefly, 2.5 mL of methanolic solution of the free radical DPPH (4%) was added to 0.1 mL of increased concentrations of RSAqEx (0.62; 1.25; 5; 10; 20; 40; 80 and 120 µg/mL).…”

“…Renal failure is quite often associated with sepsis, diabetes, and cardiovascular diseases 5 . Latest studies have documented that oxidative stress is highly prevalent in patients with renal disease 6 .…”

Acute and Subacute Toxicity and Cytotoxicity of Opuntia Dillenii (Ker-Gawl) Haw. Seed Oil and Its Impact on the Isolated Rat Diaphragm Glucose Absorption

“…Additionally, the oil is characterized by the presence of other substances such as phenols, well known for their health benefit [ 3 , 4 ]. Several kinds of research on this plant’s fruit seed oil have shown that it has numerous activities such as antioxidant [ 5 ], hepatoprotective [ 6 ], antilipidemic, antidiabetic, and antidiabetogenic [ 7 , 8 ].…”

Opuntia dillenii (Ker Gawl.) Haw., Seeds Oil Antidiabetic Potential Using In Vivo, In Vitro, In Situ, and Ex Vivo Approaches to Reveal Its Underlying Mechanism of Action

“…Previous papers have reported the potential bioeffects of ODSO, including antioxidant, anti-inflammatory, and hepatoprotective activities [ 36 , 37 ]. Furthermore, in a previous work, the antidiabetic effect of ODSO was studied, but the mechanism of action still has not been elucidated [ 38 , 39 ]. The present work aims to study the postprandial glycemic control by ODSO through an oral glycemic load shedding light on the possible mechanisms during the absorptive process by targeting digestive enzymes (α-amylase and α-glucosidase) and glucose movement across the intestinal epithelial barrier.…”