Phenolic Exudation Control and Establishment of In vitro Strawberry (Fragaria × Ananassa) cv. Chandler

Abstract

The rate of strawberry propagation through conventional technique is quite low and it is difficult to maintain planting material during the summer months under Bihar condition. Further, importing mother plants adds to the production cost. In vitro micro propagation has emerged as a potential alternative for supplying planting material for strawberry. Two type of explants viz., runner tip and nodal segment were used for the study. Phenol exudation was the major problem during establishment which caused death of majority explants. In our experiment, almost no phenolic exudation (+) and maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter. Phenolic exudation was recorded highest (++++) under control when no antioxidants were supplemented. Minimum number of days for runner tips (8.4 ± 0.23) and nodal segments (10.3 ± 0.33) taken for shoot proliferation was observed when MS medium was supplemented with activated charcoal 300 mg and 200 mg per liter, respectively. Though all other antioxidants used in our study including citric acid, PVP and activated charcoal significantly reduced oxidative browning, ascorbic acid was found to be most effective antioxidant in controlling lethal browning during in vitro establishment of strawberry. This protocol has a potential for allowing a large scale multiplication of this important crop.

Establecimiento in vitro de Fragariax ananassa var. Aroma a partir de yemas de corona

“…Esto evidenciaría que esta variable es sensible a la concentración de sales del MS y las combinaciones de los reguladores de crecimiento. Al respecto, en cv Chandler, para controlar la exudación fenólica se utilizó 300 g l -1 de ácido cítrico, donde disminuyó a cero, pero no favoreció la supervivencia de los explantes y por ende la proliferación de brotes; pero, la proliferación de brotes fue favorecida, cuando el medio MS fue suplementado con 200 a 300 g l -1 de carbón activado (Mir et al, 2019); sin embargo, en el presente trabajo se utilizó 300 mg l -1 de Ácido Ascórbico y 100 mg l -1 Ácido Cítrico, por un tiempo de inmersión de 10 minutos, en el proceso de la desinfección.…”

“…Durante la propagación in vitro, uno de los problemas a enfrentar es el establecimiento, debido al proceso de oxidación de los explantes, que causa un oscurecimiento del tejido, en especial en las especies leñosas (Azofeifa-Delgado, 2009), que se produce en todos los tejidos frente a un estrés abiótico, como un sistema de defensa del explante (Hernández & González, 2010); otro es, la contaminación por hongos, que depende del tipo de desinfectante que se utilice; así, al utilizar hipoclorito de sodio, éste no controló comparado al bicloruro de mercurio donde su porcentaje fue menor y depende de la concentración y el tiempo de exposición (García et al, 2015), una forma de reducir la contaminación por hongos, es la aplicación de fungicidas a las plantas donadoras (Bogado et al, 2016); asimismo, se tiene que ver la composición del medio, la especie, condiciones ambientales y los reguladores de crecimiento (Twaij et al, 2020). Uno de los grandes problemas que se enfrenta el cultivo de fresa, es que las plantas obtenidas mediante el sistema convencional son muy propensas a enfermedades (Dutta & Sen, 2019), de modo que para un alto rendimiento, se requiere contar con semilla que garantice la producción; la que se puede lograr mediante el cultivo in vitro (Mir et al, 2019), que permite tener la variedad o cultivar libre de patógenos en especial virus, al mismo tiempo, una menor aplicación de pesticidas; por el otro lado, obtener individuos con cierta homogeneidad, consecuencia de una propagación asexual. En este contexto, el laboratorio de Biotecnología Vegetal de la Universidad Nacional Daniel Alcides Carrión (Undac), Filial Oxapampa, requiere contar con protocolos desarrollados para cada fase que implica el cultivo in vitro de fresa.…”

Production of in vitro strawberry (Fragaria × ananassa) plantlets in large-scale system supplemented with silver nanoparticles

“…Điều này có thể giải thích là do trong quá trình sinh trưởng và phát triển, mẫu cấy sản sinh ra và tích lũy khí ethylene quá mức trong các bình nuôi cấy kín (Gaspar et al, 1992). Nhằm khắc phục các bất thường trong vi nhân giống, gia tăng sinh trưởng và phát triển để nâng cao chất lượng cây giống, một số kĩ thuật như sử dụng bình thoáng khí và bổ sung than hoạt tính vào môi trường nuôi cấy đã được nghiên cứu (Hdider, Desjardins, 1993;Mir et al, 2019). Tuy nhiên, một số hiện tượng bất thường của cây Dâu tây vẫn chưa được giải quyết triệt để.…”

Managed bees as pollinators and vectors of bio control agent against grey mold disease in strawberry plantations

“…Garden strawberry, Fragaria x ananassa Duch, is a perennial, herb that belongs to the phylum Spermatophyte and family Rosaceae. The strawberry does well in tropical and subtropical regions of the world (Mir et al, 2019). Strawberry fruits are rich in well-balanced sugars and organic acids Guimarães et al (2016), hence very nutritious for human consumption (USDA, 2015).…”