The rate of strawberry propagation through conventional technique is quite low and it is difficult to maintain planting material during the summer months under Bihar condition. Further, importing mother plants adds to the production cost. In vitro micro propagation has emerged as a potential alternative for supplying planting material for strawberry. Two type of explants viz., runner tip and nodal segment were used for the study. Phenol exudation was the major problem during establishment which caused death of majority explants. In our experiment, almost no phenolic exudation (+) and maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter. Phenolic exudation was recorded highest (++++) under control when no antioxidants were supplemented. Minimum number of days for runner tips (8.4 ± 0.23) and nodal segments (10.3 ± 0.33) taken for shoot proliferation was observed when MS medium was supplemented with activated charcoal 300 mg and 200 mg per liter, respectively. Though all other antioxidants used in our study including citric acid, PVP and activated charcoal significantly reduced oxidative browning, ascorbic acid was found to be most effective antioxidant in controlling lethal browning during in vitro establishment of strawberry. This protocol has a potential for allowing a large scale multiplication of this important crop.
Guava is one of the most promising fruit crops of India and is considered to be one of the exquisite nutritionally valuable and remunerative crops. We are unaware of any report describing macro and micronutrient dynamics in fruit at different growth stages of guava. For conducting this experiment fruit of variety Allahabad Safeda, L-49, Lalit, Shweta, Arka Kiran, Salithong, Kimchu were collected at different stages like marble, seed hardening and harvest stage for estimation of primary nutrient (N, P, K), secondary nutrient (Ca, Mg) and micronutrient (Fe, Mn, Zn, Cu). As regards the availability of mineral nutrient contents of varieties did not follow definite trend. The nutrient content particularly N, and K were recorded highest at marble stage while as Mg and Ca was accumulated maximum in stone hardening stage and P, Zn, Fe, Cu, Mg and Mn was in harvesting stage. There was least variation was observed in Mg content. Finally, it may be concluded that for the improvement of yields and quality requirement of specific mineral and nutrients is required at different growth stages.
An experiment was conducted on litchi cv. China at Horticulture garden in the Department of Horticulture (Fruit and Fruit Technology), Bihar Agricultural University, Sabour, Bhagalpur, Bihar, to show the shelf life and biochemical changes of litchi during storage. The experiment was laid out in randomized block design with five treatments and four replications. Salicylic acid was sprayed twice i.e. 15 days and 30 days before anticipated harvesting time with different concentrations of salicylic acid (T1- 50 ppm, T2-100 ppm, T3-150 ppm, T4-200 ppm and T5-control). Different concentrations have different effect on shelf life and biochemical parameters. Salicylic acid with T3-150 ppm has increased TSS (20.02 0Brix), total sugar (13.10%), ascorbic acid (38.21 mg/100 g pulp), anthocyanin (8.06 mg/100g pulp) and shelf life (4 days) on the 6th day of storage at ambient condition.
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