Phenolic Profile, Toxicity, Enzyme Inhibition, In Silico Studies, and Antioxidant Properties of Cakile maritima Scop. (Brassicaceae) from Southern Portugal

Placines, Chloé; Castañeda-Loaiza, Viana; Rodrigues, Maria João; Cg, Pereira; Stefanucci, Azzurra; Mollica, Adriano; Zengin, Gökhan; Llorent‐Martínez, Eulogio J.; Castilho, Paula C.; Custódio, Luísa

doi:10.3390/plants9020142

Cited by 30 publications

(18 citation statements)

References 54 publications

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“…When plants are subjected to salt stress, they can resist or reduce the damage caused by increasing osmotic adjustment, maintaining cell wall integrity and improving ROS scavenging [ 50 , 51 , 52 ]. Several studies have highlighted that phenolic compounds maintain high antioxidant activity and are involved in ROS detoxification and prevention from oxidative damage under salt stress [ 53 , 54 , 55 ]. More specifically, lignans are involved in lignification and cell wall synthesis and they exhibit antioxidant capacity, thus they have a positive role in stress tolerance [ 39 , 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Salinity Stress Alters the Secondary Metabolic Profile of M. sativa, M. arborea and Their Hybrid (Alborea)

Sarri

Termentzi

Abraham

et al. 2021

IJMS

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Increased soil salinity, and therefore accumulation of ions, is one of the major abiotic stresses of cultivated plants that negatively affect their growth and yield. Among Medicago species, only Medicago truncatula, which is a model plant, has been extensively studied, while research regarding salinity responses of two important forage legumes of Medicago sativa (M. sativa) and Medicago arborea (M. arborea) has been limited. In the present work, differences between M. arborea, M. sativa and their hybrid Alborea were studied regarding growth parameters and metabolomic responses. The entries were subjected to three different treatments: (1) no NaCl application (control plants), (2) continuous application of 100 mM NaCl (acute stress) and (3) gradual application of NaCl at concentrations of 50-75-150 mM by increasing NaCl concentration every 10 days. According to the results, M. arborea maintained steady growth in all three treatments and appeared to be more resistant to salinity. Furthermore, results clearly demonstrated that M. arborea presented a different metabolic profile from that of M. sativa and their hybrid. In general, it was found that under acute and gradual stress, M. sativa overexpressed saponins in the shoots while M. arborea overexpressed saponins in the roots, which is the part of the plant where most of the saponins are produced and overexpressed. Alborea did not perform well, as more metabolites were downregulated than upregulated when subjected to salinity stress. Finally, saponins and hydroxycinnamic acids were key players of increased salinity tolerance.

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Section: Discussionmentioning

confidence: 99%

Salinity Stress Alters the Secondary Metabolic Profile of M. sativa, M. arborea and Their Hybrid (Alborea)

Sarri

Termentzi

Abraham

et al. 2021

IJMS

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“…All metabolites, which were not abundant in at least 80 % of the samples in at least one of the eight origin × water treatment × temperature treatment combinations, were removed from the dataset for further analyses, resulting in a total number of 1966 metabolites in the final merged dataset. Annotation of these metabolites was performed with MetaboScape 2021b based on the comparison of mass spectra and m/z ratios with the KEGG database (Kanehisa et al, 2019), HMDB (Wishart et al, 2007), Lotus database (Rutz et al, 2022) and various original research papers (e.g., Rodman 1976;Radwan et al 2008;Taamalli et al 2015;Omer et al 2016Omer et al , 2019Arbelet-Bonnin et al 2020;Placines et al 2020;Shams et al 2010).…”

Section: Metabolome Traitsmentioning

confidence: 99%

A metabolomic view on local climate adaptation: Latitudinal divergence of heat and drought responses in a coastal plant

Schrieber

Gluesing

Peters

et al. 2022

Preprint

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Studying natural variation in multi-stress resistance is central for predicting and managing the population dynamics of wild plant species under rapid global change. Yet, it remains a challenging goal in this field to integrate knowledge on the complex biochemical underpinnings for the targeted non-model species. Here, we studied latitudinal divergence in combined drought and heat stress resistance in European populations of the dune plant Cakile maritima, by combining comprehensive plant phenotyping with metabolic profiling via FT-ICR-MS and UPLC-TQ-MS/MS. We observed pronounced constitutive divergence in growth phenology, leaf functional traits and defence chemistry (glucosinolates, alkaloids) among population origins. Most importantly, the magnitude of growth reduction under stress was partly weaker in southern plants and associated with divergence in plastic growth responses (root expansion, leaf abscission) and the stress-induced modulation of primary and specialized metabolites with known central functions not only in plant abiotic but also biotic stress resistance. Our study supports that divergent selection has shaped the constitutive and stress-induced expression of numerous morphological and biochemical functional traits to mediate higher abiotic stress resistance in southern Cakile populations, and highlights that metabolomics is a powerful tool to explore the mechanistic underpinnings of local stress adaptation in non-model species.

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“…The raw structure has been polished with UCSF Chimera [32] by removing additional chain, and the B chain was used as crystallographic protein complex. The protein was prepared with the Protein Preparation Wizard [33] implemented in Maestro suite 11.1 [34], by filling missing side chain and loops e-converting seleno-methionines and seleno-cysteines to methionines and cysteines, respectively, generating heteroatoms states at pH 7.4 using Prime [35], using a well-established procedure previously reported by our research group [36][37][38][39] then deleting non-catalytic waters and other co-crystallized molecule, were conducted the H-bond assignment by using PROpKA at pH 7.4, and a minimization of only the hydrogens. The copper ions present in the catalytic pocket of tyrosinase, have been optimized by the automatic generation of optimized metal states.…”

Section: Computational Tests 281 Receptor Preparationmentioning

confidence: 99%

LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds

et al. 2021

Self Cite

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The impact of two extraction solvents on the phenolic composition, antioxidant, and enzymes inhibitory and antimicrobial activities of two parts (leaves and stem bark) of P. kotschyi was studied. Two different LC-DAD-MSn approaches were used to identify and quantify the bioactive compounds in the different extracts. A total of thirty-two compounds were quantified, being the procyanidin the most abundant in stem bark while catechin and flavonoids are most abundant in leaves. Overall, the stem bark extraction using methanol showed higher amounts of total phenolic (131.83 ± 1.81 mg GAE/g) and flavanol (14.14 ± 0.11 mg CE/g) while the leaves extraction using water exhibited stronger levels of total flavonoid (44.95 ± 0.38 mg RE/g) and phenolic acid (63.58 ± 2.00 mg CAE/g). As regards the antioxidant assays, methanol stem bark extracts were characterized by the highest antioxidant activities (DPPH: 1.94 ± 0.01 mmol TE/g, ABTS: 3.31 ± 0.01 mmol TE/g, FRAP: 2.86 ± 0.02 mmol TE/g, CUPRAC: 5.09 ± 0.08 mmol TE/g, phosphomolybdenum: 5.16 ± 0.23 mmol TE/g and metal chelating: 17.12 ± 0.46 mg EDTAE/g). In addition, the methanolic extracts of stem bark had highest impact on acetylcholinesterase (2.54 mg GALAE/g), butyrylcholinesterase (5.48 mg GALAE/g). In contrast, the methanolic extracts of leaves was potent against tyrosinase (77.39 ± 0.21 mg KAE/g) and α-glucosidase (0.97 ± 0.01 mmol ACAE/g), while a higher anti-α–amylase (0.97 ± 0.01 mmol ACAE/g) was observed for water extracts of the same part. All of the tested extracts showed inhibitory effects on elastase, except methanolic leaves extracts. Additionally, the extracts exhibited appreciable antifungal toward A. ochraceus, A. fumigatus, P. ochrochloron, T. viride, and P. funiculosum and promising antibacterial activity against M. flavus, S. aureus, L. monocytogenes, E. coli, P. aeruginosa, E. cloacae, and S. typhimurium. Taken together, the outcomes demonstrated P. kotschyi as a novel source of bioactive molecules of interest with an evident therapeutic value.

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Phenolic Profile, Toxicity, Enzyme Inhibition, In Silico Studies, and Antioxidant Properties of Cakile maritima Scop. (Brassicaceae) from Southern Portugal

Cited by 30 publications

References 54 publications

Salinity Stress Alters the Secondary Metabolic Profile of M. sativa, M. arborea and Their Hybrid (Alborea)

Salinity Stress Alters the Secondary Metabolic Profile of M. sativa, M. arborea and Their Hybrid (Alborea)

A metabolomic view on local climate adaptation: Latitudinal divergence of heat and drought responses in a coastal plant

LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds

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