Phenotype of NK Cells and Cytotoxic/Apoptotic Mediators Expression in Ectopic Pregnancy

Laškarin, Gordana; Redžović, Arnela; Vukelić, Petar; Veljković, Danijela; Gulić, Tamara; Haller, Herman; Rukavina, Daniel

doi:10.1111/j.1600-0897.2010.00844.x

Cited by 28 publications

(29 citation statements)

References 37 publications

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“…These cells are also absent in Fallopian tube from ectopic pregnancy (Vassiliadou and Bulmer, 1998; von Rango et al, 2001). However, similar to a recent study (Laskarin et al, 2010), we found substantial numbers of CD3−CD56 dim CD16− NK cells in Fallopian tube from non-pregnant women. Little is known about the function of this NK cell subtype and in the peripheral blood these cells account for less than 1% of all mononuclear cells (Lanier et al, 1986).…”

Section: Discussionsupporting

confidence: 92%

“…In Fallopian tube from women with ectopic pregnancy, CD3−CD56 bright CD16− NK cells have been shown to be absent from the tubal implantation site (Proll et al, 2000; Vassiliadou and Bulmer, 1998; von Rango et al, 2001). However, a population of CD3−CD56 dim CD16− NK cells has recently been reported in Fallopian tube from women with ectopic pregnancy (Laskarin et al, 2010). The numbers of CD8+ lymphocytes and macrophages have been shown to be elevated at the tubal implantation site compared with elsewhere in the same Fallopian tube (Ulziibat et al, 2006; von Rango et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Lymphoid and myeloid cell populations in the non-pregnant human Fallopian tube and in ectopic pregnancy

Shaw

Fitch

Cartwright

et al. 2011

Journal of Reproductive Immunology

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Lymphoid and myeloid cell populations in human endometrium are well-documented and are known to play important roles in providing immune tolerance, controlling trophoblast invasion, and mediating vascular remodeling. Immune cell populations in the Fallopian tube have not been comprehensively studied. The aim of this study was to characterize lymphoid and myeloid cell populations in non-pregnant Fallopian tube and determine whether they are altered in Fallopian tube from women with ectopic pregnancy. Fallopian tube was analyzed by flow cytometry and immunohistochemistry. Populations of CD3+ (CD4+ and CD8+) lymphocytes, LIN1−HLADR+ (CD123+ and CD11c+) dendritic cells, monocytes, neutrophils, and CD56dimCD16− natural killer (NK) cells were demonstrated to be present in non-pregnant Fallopian tube. CD123+ dendritic cells were predominant over CD11c+ dendritic cells. Numbers of CD11c+ cells were significantly higher in the progesterone-dominant mid-luteal phase of the menstrual cycle compared with the follicular phase. Numbers of CD45+ leukocytes, CD68+ cells, and CD11c+ cells were higher in Fallopian tube from women with ectopic pregnancy compared with mid-luteal phase Fallopian tube. These data will advance our understanding of normal human Fallopian tube physiology and disorders of Fallopian tube function, such as ectopic pregnancy.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Lymphoid and myeloid cell populations in the non-pregnant human Fallopian tube and in ectopic pregnancy

Shaw

Fitch

Cartwright

et al. 2011

Journal of Reproductive Immunology

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show abstract

“…Isolation of cells from decidual tissue has been described in detail elsewhere (Laskarin et al, 2010). Briefly, decidual tissue was thoroughly rinsed, cut into pieces, and exposed to enzymatic digestion using 0.1% collagenase IV (Sigma, Munich, Germany).…”

Section: Isolation Of Decidual Cells and Peripheral Blood Lymphocytesmentioning

confidence: 99%

“…These cells are heavily endowed with the mediators of cytolytic potential like granulysin (GNLY) and perforin (Veljkovic Vujaklija et al, 2011). Their peripheral blood NK counterparts have a different phenotype (CD56 dim CD16 + ) (Laskarin et al, 2010). More than 85% of the uterine CD56 + NK cells express the GNLY molecule, as well as more than 90% of peripheral blood CD56 + NK cells (Veljkovic Vujaklija et al, 2011).…”

Section: Introductionmentioning

confidence: 98%

Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface

Vujaklija¹,

Dominović²,

Gulić³

et al. 2013

Journal of Reproductive Immunology

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“…Our recent results show that first trimester pregnancy decidual tissue is diffusely infiltrated by granulysin-positive cells which accumulate around uterine glands and blood vessels [64, 65]. Granulysin protein was also found in peripheral blood lymphocytes of pregnant women but in significantly lower amounts [64].…”

Section: Granulysin At the Maternal-foetal Interface: A Double-edgmentioning

confidence: 99%

Cell Death Mechanisms at the Maternal-Fetal Interface: Insights into the Role of Granulysin

Vujaklija

Sučić

Gulić

et al. 2012

Clinical and Developmental Immunology

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During mammal pregnancy, a sensitive balance between hormones, cytokines, humoral factors, and local cellular interactions must be established. Cytotoxic cells infiltrating the decidua are heavily equipped with cytolytic molecules, in particular perforin and granulysin. Granulysin is especially abundant in NK cells which are able to spontaneously secrete high quantities of granulysin. Besides being a potent bactericidal and tumoricidal molecule, granulysin is also found to be a chemoattractant and a proinflammatory molecule. The precise role(s) of granulysin at the maternal-fetal interface has not been elucidated yet. It is possible that it behaves as a double-edged sword simultaneously acting as an immunomodulatory and a host defense molecule protecting both the mother and the fetus from a wide spectrum of pathogens, and on the other hand, in case of an NK cell activation, acting as an effector molecule causing the apoptosis of semiallograft trophoblast cells and consequently leading to various pregnancy disorders or pregnancy loss.

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Phenotype of NK Cells and Cytotoxic/Apoptotic Mediators Expression in Ectopic Pregnancy

Abstract: Authentic uNK cell population seems to be insufficient to restrict trophoblast invasion because of low expression of cytotoxic/apoptotic mediators.

Cited by 28 publications

References 37 publications

Lymphoid and myeloid cell populations in the non-pregnant human Fallopian tube and in ectopic pregnancy

Lymphoid and myeloid cell populations in the non-pregnant human Fallopian tube and in ectopic pregnancy

Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface

Cell Death Mechanisms at the Maternal-Fetal Interface: Insights into the Role of Granulysin

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