Phenotypes, antibacterial-resistant profile, and virulence-associated genes of Salmonella serovars isolated from retail chicken meat in Egypt

Awad, Amal; Gwida, Mayada; Khalifa, Eman; Sadat, Asmaa

doi:10.14202/vetworld.2020.440-445

Cited by 21 publications

(22 citation statements)

References 33 publications

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“…In the present study, 8 out 100 chicken samples were positive for S. Enterica representing (8%); this result is in good harmony with a study applied by [ 30 , 31 ], who reported (8.6% and 8%), respectively, in the chicken farms. Three serovars were identified in chicken samples which included S. Kentucky 25% (2/8), which was close to [ 32 , 33 ], and S. Typhimurium 37.5% (3/8) and S. Enteritidis 37.5% (3/8), which were quite similar to [ 33 , 34 ]. The prevalence of Salmonella from 100 fecal calves’ samples was 2% (2 isolates), which was close to that obtained from (1.5%) [ 35 ].…”

Section: Discussionmentioning

confidence: 95%

Genetic Relationship between Salmonella Isolates Recovered from Calves and Broilers Chickens in Kafr El-Sheikh City Using ERIC PCR

Tawfik

Gawish

Abotaleb

et al. 2022

Animals

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A prevalent bacterial intestinal infection with severe economic damage is salmonellosis. Our study was carried out to diagnose Salmonella from chickens and calves, to determine its resistance to antimicrobials’ phenotypic and genotypic characterization of integrons and β lactamase genes in the multidrug resistance of different Salmonella serotypes, and to detect the genetic relationship between Salmonella isolates collected from different origins using an ERIC PCR. In total, 200 samples from diseased chicken and diarrheic calves were obtained from 50 various farms from Kafr El-sheikh, Egypt. Salmonella poultry isolates were characterized as S. Typhimurium (3/8), S. Enteritidis (3/8), and S. Kentucky (2/8), but Salmonella isolates from cattle were S. Enteritidis (1/2) and S. Kentucky (1/2). When antibiotic susceptibility testing was completed on all of the isolates, it showed that there was multidrug resistance present (MDR). A PCR was applied for identifying the accompanying class 1 integrons and ESBLs from MDR Salmonella isolates (two isolates of S. Kentucky were divided as one from calf and one from poultry). Our results detected blaTEM and class 1 integron, but were negative for bla IMP, bla VIM, and bla SHV. An ERIC PCR was conducted for understanding the clonal relation between various β-lactamase-producing MDR Salmonella isolates. The same four previously mentioned isolates were also tested. The two isolates of S. Enteritidis isolated from poultry and calves had 100% similarity despite indicating that there were interactions between broilers and calves living on the same farm that caused infection from the same Salmonella strains, while the other two isolates of S. Kentucky showed only 33% serovarities.

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Section: Discussionmentioning

confidence: 95%

Genetic Relationship between Salmonella Isolates Recovered from Calves and Broilers Chickens in Kafr El-Sheikh City Using ERIC PCR

Tawfik

Gawish

Abotaleb

et al. 2022

Animals

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“…Moreover, it was confirmed that S. kentucky (C3 serogroup) had been identified as one of the most prominent Salmonella serovars isolated from broilers causing diarrhea and high mortalities in Egypt (Mahmoud et al 2018). Additionally, it was reported that both Salmonella enteritidis and Salmonella kentucky were the dominant serovars with 22.6% each (Awad et al 2020). However, the percent of S. enteritidis, S. virchow and S. kentucky was reported to be 2.4%, 1.4% and 0.8% respectively (Sedeik et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…European Food Safety Authority (EFSA) stated the prevalence of S. infantis (29.2%) and S. enteritidis (13.6%), followed by S. kentucky (6.2%) and S. typhimurium (4.4%) as the most isolated Salmonella serotypes in broiler chickens in European union (EFSA, 2010). Furthermore, different Salmonella serovars have been reported in Egypt (Abd El-Ghany et al 2012;Halawa et al 2016;El-Sharkawy et al 2017;Mahmoud et al 2018;Elkenany et al 2019;Awad et al 2020). Thus, continuous isolation and characterization of the most prevalent serovars are essential for improvement of salmonellosis prevention and reduce its public health hazard.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Serological Identification of Current Salmonella Species Recovered from Broiler Chickens in Egypt

2022

Int J Vet Sci

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This study was carried out to identify and serotype Salmonella (S.) species from suspected broiler chickens and healthy chickens in seven Egyptian governorates (KafrEl-sheikh, Fayuom, Qalubia, Menofiya, Garbia, Cairo, and Giza). Five hundred specimens (liver, spleen, caecum, and bone marrow) were collected from poultry farms and slaughterhouses. Samples were only collected from slaughterhouses in Cairo and Giza governorates. Forty-nine Salmonella isolates were recovered, and their colonies were characterized on specific media. Those Salmonella isolates were morphologically and biochemically identified. Furthermore, 33 out of 500 samples were serologically confirmed as Salmonella serovars by the slide agglutination test based on O and H antigen using 140 commercial polyvalent and monovalent diagnostic Salmonella antisera with a prevalence rate of 6.6%. They were serotyped as S. blegdam (39.4%), S. typhimurium (21.2%), S. montevideo (9.1%), S. agama (6.1%), S. gueuletapee (6.1%), S. salamae (6.1%), S. enteritidis (3%), S. infantis (3%), S. kentucky (3%), and S. virchow (3%). The prevalence of Salmonella serovars in KafrEl-sheikh, Fayoum, Qalubia, Menofiya, Garbia, Cairo, and Giza was 8.5%, 4%, 8%, 12%, 8%, 0%, and 0%, respectively. Finally, continuous monitoring of Salmonella serovars in chickens is essential for better prevention and reduction of its zoonotic risk.

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“…Moreover, the multi-drug resistant (MDR) strains of salmonella are emerging and their prevalence is also increasing day by day (118)(119)(120). In Egypt, the percentage of prevalence of multi drug resistant S. enterica was found to be 69.8% in the marketed meat samples in 2010, and 82.4 to 100% in 2020 (121)(122)(123).…”

Section: Salmonella Enteritidismentioning

confidence: 99%

Cell membrane-coated nanoparticles: An emerging antibacterial platform for pathogens of food animals

Altaf

Alkheraije

2023

Front. Vet. Sci.

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Bacterial pathogens of animals impact food production and human health globally. Food animals act as the major host reservoirs for pathogenic bacteria and thus are highly prone to suffer from several endemic infections such as pneumonia, sepsis, mastitis, and diarrhea, imposing a major health and economical loss. Moreover, the consumption of food products of infected animals is the main route by which human beings are exposed to zoonotic bacteria. Thus, there is excessive and undue administration of antibiotics to fight these virulent causative agents of food-borne illness, leading to emergence of resistant strains. Thus, highprevalence antibiotic-resistant resistant food-borne bacterial infections motivated the researchers to discover new alternative therapeutic strategies to eradicate resistant bacterial strains. One of the successful therapeutic approach for the treatment of animal infections, is the application of cell membrane-coated nanoparticles. Cell membranes of several different types of cells including platelets, red blood cells, neutrophils, cancer cells, and bacteria are being wrapped over the nanoparticles to prepare biocompatible nanoformulations. This diversity of cell membrane selection and together with the possibility of combining with an extensive range of nanoparticles, has opened a new opportunistic window for the development of more potentially effective, safe, and immune evading nanoformulations, as compared to conventionally used bare nanoparticle. This article will elaborately discuss the discovery and development of novel bioinspired cell membrane-coated nanoformulations against several pathogenic bacteria of food animals such as Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Campylobacter jejuni, Helicobacter pylori, and Group A Streptococcus and Group B Streptococcus.

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Phenotypes, antibacterial-resistant profile, and virulence-associated genes of Salmonella serovars isolated from retail chicken meat in Egypt

Cited by 21 publications

References 33 publications

Genetic Relationship between Salmonella Isolates Recovered from Calves and Broilers Chickens in Kafr El-Sheikh City Using ERIC PCR

Genetic Relationship between Salmonella Isolates Recovered from Calves and Broilers Chickens in Kafr El-Sheikh City Using ERIC PCR

Isolation and Serological Identification of Current Salmonella Species Recovered from Broiler Chickens in Egypt

Cell membrane-coated nanoparticles: An emerging antibacterial platform for pathogens of food animals

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