Phenotypes of Human Native Natural Killer (NK) Cells and MLC-Activated NK-Like Cells

Rumpold, Helmut; Obexer, G; Möschl, P

doi:10.1016/b978-0-08-030764-0.50194-4

Cited by 2 publications

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References 8 publications

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“…Two molecules have been shown to represent pan NK-associated antigens with reasonably selective expression:. a 60-kDa Fc receptor (also present on granulocytes) recognized by a series of reagents such as VEP13 [9], B73.1 [lo], LEU11 [ l l ] and a 200-220-kDa molecule, of unknown function, defined by anti-N901 [12] and anti-NKHlA [13] antibodies. The latter mAb react with virtually all circulating NK cells in a resting state and all cloned lines of activated NK cells that we have developed thus far [13].…”

Section: Introductionmentioning

confidence: 99%

Proliferative responses of circulating human NK cells: delineation of a unique pathway involving both direct and helper signals

et al. 1985

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The present studies were performed to investigate mechanisms of human natural killer (NK) cell activation. NK-active cells were purified out of heterogeneous large granular lymphocytes (LGL)-enriched suspensions using a "pan NK"-specific monoclonal antibody termed anti-NKH1A. It was found that treatment of NKH1A+-sorted cells by T lymphocyte mitogens such as phytohemagglutinin (PHA) or anti-T11(2) plus anti-T11(3) did not induce proliferative responses. In fact, there was no measurable interleukin 2 (IL2) secretion and no significant increase in IL2 susceptibility following incubation with either PHA or anti-T11(2) plus anti-T11(3). However, as opposed to small resting T lymphocytes, NKH1A+ cells moderately proliferated in the presence of IL2. This IL2-dependent proliferation was dramatically increased after interaction between NK-active lymphocytes and certain hematopoietic cell lines such as K562 or EBV-transformed lymphoblastoid cell lines. These studies indicate that unique activation mechanisms can be identified when NK lymphocytes are purified out of LGL-enriched fractions. The NK activation pathway delineated here appears essentially distinct from those described for T lymphocytes. Indeed the nature of the NK cell/inducing cell interaction is unknown and triggering cannot be related, for example, to a conventional allogeneic effect mediated through membrane exposure of class I or class II major histocompatibility complex gene products. Moreover, these interactions do not lead to the development of autocrine responses. Together the present results support the view that direct signals can preactivate NK cells but are not sufficient to trigger proliferation which must therefore be regulated by helper populations.

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Section: Introductionmentioning

confidence: 99%

Proliferative responses of circulating human NK cells: delineation of a unique pathway involving both direct and helper signals

et al. 1985

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Patients with a deficiency of natural killer cell activity lack the VEP13-positive lymphocyte subpopulation

Ziegler‐Heitbrock

Rumpold

Wagenpfeil³

et al. 1985

Blood

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Many patients with B-type chronic lymphocytic leukemia (CLL) exhibit a profound defect in their natural killer (NK) cell activity, the basis of which is still obscure. Hence, we analyzed the NK cells from peripheral blood samples from 11 patients with CLL for phenotype and function, after removal of the leukemic cells with a monoclonal antibody (BA-1) plus complement. Phenotypic analysis of these nonleukemic cells with monoclonal antibodies (MoAbs) against NK cells revealed that the CLL patients had higher percentages of HNK-1-positive cells (23.5% compared to controls with 14.7%). In contrast, VEP13- positive cells were absent or low in seven patients (0.8% compared to controls with 11.2%) and normal in four patients (10.5%). When testing NK cell activities against K562 or MOLT 4 target cells, patients with no or minimal numbers of VEP13-positive cells were found to be deficient, while patients with normal percentages of VEP13-positive cells had NK cell activity comparable to controls. Isolation by fluorescence-activated cell sorter of HNK-1-positive cells from patients lacking VEP13-positive cells and NK cell activity indicated that the majority of the HNK-1-positive cells in these patients had the large granular lymphocyte morphology that is characteristic of NK cells. Thus, the deficiency of NK cell activity in CLL patients appears to result from the absence of cells carrying the VEP13 marker.

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Phenotypes of Human Native Natural Killer (NK) Cells and MLC-Activated NK-Like Cells

Cited by 2 publications

References 8 publications

Proliferative responses of circulating human NK cells: delineation of a unique pathway involving both direct and helper signals

Proliferative responses of circulating human NK cells: delineation of a unique pathway involving both direct and helper signals

Patients with a deficiency of natural killer cell activity lack the VEP13-positive lymphocyte subpopulation

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