Phenotypic Analysis of Urothelial Exfoliated Cells in Bladder Cancer via Microfluidic Immunoassays: Sialyl-Tn as a Novel Biomarker in Liquid Biopsies

Carvalho, Sónia; Abreu, Catarina M.; Ferreira, Dylan; Lima, Luís; Ferreira, José Alexandre; Santos, Lúcio Lara; Ribeiro, Ricardo; Grenha, V.; Martínez‐Fernández, Mónica; Dueñas, Marta; Suárez-Cabrera, Cristian; Paramio, Jesús M.; Diéguez, Lorena; Freitas, P. P.; Oliveira, Marta I.

doi:10.3389/fonc.2020.01774

Cited by 11 publications

(7 citation statements)

References 58 publications

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“…Indeed, Carvalho et al. (2020) ( 74 ) recently reported an association between STn expression and advanced bladder cancer stage and grade. However, the correlation between the STn antigen and prognosis seems to be rather ambiguous and cancer type-specific ( 64 ).…”

Section: Stn and O-gd2 The Results Of Alternative Glycosylation Proce...mentioning

confidence: 99%

CD44v6, STn & O-GD2: promising tumor associated antigens paving the way for new targeted cancer therapies

Lodewijk,

Dueñas,

Paramio

et al. 2023

Front. Immunol.

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Targeted therapies are the state of the art in oncology today, and every year new Tumor-associated antigens (TAAs) are developed for preclinical research and clinical trials, but few of them really change the therapeutic scenario. Difficulties, either to find antigens that are solely expressed in tumors or the generation of good binders to these antigens, represent a major bottleneck. Specialized cellular mechanisms, such as differential splicing and glycosylation processes, are a good source of neo-antigen expression. Changes in these processes generate surface proteins that, instead of showing decreased or increased antigen expression driven by enhanced mRNA processing, are aberrant in nature and therefore more specific targets to elicit a precise anti-tumor therapy. Here, we present promising TAAs demonstrated to be potential targets for cancer monitoring, targeted therapy and the generation of new immunotherapy tools, such as recombinant antibodies and chimeric antigen receptor (CAR) T cell (CAR-T) or Chimeric Antigen Receptor-Engineered Natural Killer (CAR-NK) for specific tumor killing, in a wide variety of tumor types. Specifically, this review is a detailed update on TAAs CD44v6, STn and O-GD2, describing their origin as well as their current and potential use as disease biomarker and therapeutic target in a diversity of tumor types.

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Section: Stn and O-gd2 The Results Of Alternative Glycosylation Proce...mentioning

confidence: 99%

CD44v6, STn & O-GD2: promising tumor associated antigens paving the way for new targeted cancer therapies

Lodewijk,

Dueñas,

Paramio

et al. 2023

Front. Immunol.

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“…The proposed biosensor is applicable to a variety of applications, such as biomarkers for a specific type of cancer, or biomarkers for detecting multiple types of cancer. The advanced version of the sensor could also be implemented using microfluidic sensing technology [37].…”

Section: Discussionmentioning

confidence: 99%

Biological sensing using anomalous hall effect devices

Ramesh¹,

Chou²,

Lu³

et al. 2022

Nanotechnology

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This paper outlines an approach to biological sensing involving the use of spintronic devices to sense magnetic nanoparticles attached to biological carriers. We developed an enzyme-linked immunosorbent assay (ELISA)-based Anomalous Hall Effect magnetic sensor via surface functionalization using Triethoxysilylundecanal (TESUD). The proposed sensor uses a CoFeB/MgO heterostructure with a perpendicular magnetic anisotropy. Through several sets of magnetic layer thickness, this work also explored the optimization process of ferromagnetic layer used. Our spintronics-based bio sensor is compatible with semiconductor fabrication technology and can be effectively miniaturized to integrate with semiconductor chips, which has the advantage of reduced manufacturing cost and reduced power consumption. The proposed sensor provides real-time measurement results and it is competitive to conventional biological colorimetric measurement systems in terms of accuracy and immediacy.

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“…The device provided the first glycomic profile of colorectal CTCs. Following a similar concept, a "UriChip" was later developed to enrich and profile exfoliated tumor cells of bladder cancer patients (Carvalho et al, 2020). On-chip immunocapture of circulating bladder cancer cells was performed using antibodies specific to aberrantly glycosylated integrin (Wang Glycoprofiling of gastric disease states using a lectin-functionalized microfluidic.…”

Section: Microfluidics-assisted Glycoprofiling With Carbohydrate-bind...mentioning

confidence: 99%

More small tools for sweet challenges: advances in microfluidic technologies for glycan analysis

Pinnock,

Carten,

Daniel

2024

Front. Lab. Chip. Technol.

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Carbohydrates, also known glycans, are ubiquitous in nature and exhibit a wide array of biological functions essential to life. Glycans often exist as conjugates of proteins or lipids and reside predominantly at the surface of cells, where their structure and composition are known to vary in a disease-dependent fashion. This observation has encouraged the development of tools for monitoring glycan patterns on individual molecules, cells, and tissues, to elucidate the links between glycosylation and disease for therapeutic and diagnostic applications. Over the past 2 decades, microfluidic technology has emerged as an advantageous tool for profiling the glycan content of biological systems. Miniaturizing carbohydrate analysis can circumvent several challenges commonly encountered with conventional-scale analytical techniques such as low throughput and poor detection sensitivity. The latter is often complicated by the low abundance of glycans in biological specimens and the complexity of carbohydrate structures, which often necessitates extensive concentration and purification of glycans to discern their structural features. We previously examined the application of microfluidics in the synthesis of carbohydrates in a recent paper (Pinnock et al., Anal. Bioanal. Chem., 2022, 414 (18), 5139–63). This review builds upon that discussion by delving into the application of microfluidics in the complementary field of carbohydrate analysis. Special attention is given to applications related to glycomics and the ways that microfluidics have enhanced the sensitivity, reproducibility, and throughput of carbohydrate identification and structural characterization.

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Phenotypic Analysis of Urothelial Exfoliated Cells in Bladder Cancer via Microfluidic Immunoassays: Sialyl-Tn as a Novel Biomarker in Liquid Biopsies

Cited by 11 publications

References 58 publications

CD44v6, STn & O-GD2: promising tumor associated antigens paving the way for new targeted cancer therapies

CD44v6, STn & O-GD2: promising tumor associated antigens paving the way for new targeted cancer therapies

Biological sensing using anomalous hall effect devices

More small tools for sweet challenges: advances in microfluidic technologies for glycan analysis

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