Phenotypic and functional characterization of Bacillus anthracis biofilms

Lee, Keehoon; Costerton, J. William; Ravel, Jacques; Auerbach, Raymond K.; Wagner, David M.; Keim, Paul; Leid, Jeff G.

doi:10.1099/mic.0.2006/003376-0

Cited by 38 publications

(39 citation statements)

References 43 publications

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“…These findings are in partial agreement with an earlier report wherein only microcolonies were observed in 48 h old vegetative Bacillus anthracis biofilm (Lee et al, 2007). With time, various microbial growth associated changes occur within a biofilm along with depletion of nutrients and accumulation of metabolic products (Hall-Stoodley & Stoodley, 2005;Hunt, Werner, Huang, Hamilton, & Stewart, 2004).…”

Section: Bgc Initiated By Different Cells Stagessupporting

confidence: 92%

Influence of physiological cell stages on biofilm formation by Bacillus cereus of dairy origin

Pagedar¹,

Singh²

2012

International Dairy Journal

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Section: Bgc Initiated By Different Cells Stagessupporting

confidence: 92%

Influence of physiological cell stages on biofilm formation by Bacillus cereus of dairy origin

Pagedar¹,

Singh²

2012

International Dairy Journal

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“…Biofilm was developed by growing the cells under static condition as described previously (Lee et al, 2007). Pellicle was allowed to form in glass tubes under static conditions.…”

Section: Biofilm and Pellicle Formationmentioning

confidence: 99%

“…Exponential phase cells from BHI culture of B. anthracis were washed by phosphate free buffer and used as inoculums for both biofilm and pellicle formation. Biofilm was visualized microscopically by nucleic acid syto9 staining method by fluorescence microscope at 20× resolution (Lee et al, 2007) and quantitatively measured by crystal violet staining as described previously (Lee et al, 2007;Peeters et al, 2008).…”

Section: Biofilm and Pellicle Formationmentioning

confidence: 99%

Phosphate starvation enhances the pathogenesis of Bacillus anthracis

Aggarwal

Somani

Bhatnagar

2015

International Journal of Medical Microbiology

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“…It has been documented that bacterial biofilms formed during chronic human infections, including recurrent otitis media, are often less susceptible to eradication by antimicrobial agents (9,11,22). Moreover, TFP have been shown to be important for the initial attachment and microcolony formation at the onset of biofilm formation by several gram-negative bacteria (20,(32)(33)(34)43).…”

Section: Contribution Of Tfp To In Vitro Adherencementioning

confidence: 99%

Contribution of Moraxella catarrhalis Type IV Pili to Nasopharyngeal Colonization and Biofilm Formation

et al. 2007

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Moraxella catarrhalis is a gram-negative mucosal pathogen of the human respiratory tract. Although little information is available regarding the initial steps of M. catarrhalis pathogenesis, this organism must be able to colonize the human mucosal surface in order to initiate an infection. Type IV pili (TFP), filamentous surface appendages primarily comprised of a single protein subunit termed pilin, play a crucial role in the initiation of disease by a wide range of bacteria. We previously identified the genes that encode the major proteins involved in the biosynthesis of M. catarrhalis TFP and determined that the TFP expressed by this organism are highly conserved and essential for natural transformation. We extended this initial study by investigating the contribution of TFP to the early stages of M. catarrhalis colonization. TFP-deficient M. catarrhalis bacteria exhibit diminished adherence to eukaryotic cells in vitro. Additionally, our studies demonstrate that M. catarrhalis cells form a mature biofilm in continuous-flow chambers and that biofilm formation is enhanced by TFP expression. The potential role of TFP in colonization by M. catarrhalis was further investigated using in vivo studies comparing the abilities of wild-type M. catarrhalis and an isogenic TFP mutant to colonize the nasopharynx of the chinchilla. These results suggest that the expression of TFP contributes to mucosal airway colonization. Furthermore, these data indicate that the chinchilla model of nasopharyngeal colonization provides an effective animal system for studying the early steps of M. catarrhalis pathogenesis.Moraxella catarrhalis, a gram-negative mucosal pathogen of the human respiratory tract, can also reside as a nasopharyngeal commensal. M. catarrhalis is capable of causing multiple diseases of the upper respiratory tract, including sinusitis, laryngitis, and acute and chronic otitis media, predominantly in pediatric populations, as well as recurrent exacerbations of chronic obstructive pulmonary disease in adults with underlying lung disease (31, 41). As with many other pathogenic bacteria, adherence to host tissues and subsequent colonization of the respiratory tract mucosa are believed to be essential prior to the development of M. catarrhalis infections.M. catarrhalis expresses a number of cell surface components that have been postulated to be involved in bacterial virulence (reviewed in references 29 and 41). However, in vivo studies of M. catarrhalis pathogenesis have been limited by the lack of an effective animal model to investigate bacterial colonization of the upper respiratory tract epithelium. Even with the most prevalently used model system, the mouse pulmonary clearance model, M. catarrhalis is markedly cleared from the site within 6 h and the organism is almost completely eradicated by 24 h (40). Although little information is available regarding the actual steps involved in the pathogenesis of M. catarrhalis infections in vivo, it is clear that this organism must attach to the mucosal surface in order to ...

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Phenotypic and functional characterization of Bacillus anthracis biofilms

Cited by 38 publications

References 43 publications

Influence of physiological cell stages on biofilm formation by Bacillus cereus of dairy origin

Influence of physiological cell stages on biofilm formation by Bacillus cereus of dairy origin

Phosphate starvation enhances the pathogenesis of Bacillus anthracis

Contribution of Moraxella catarrhalis Type IV Pili to Nasopharyngeal Colonization and Biofilm Formation

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